Bacterial dry matter content and biomass estimations

Approximately 20% dry-matter content appears to be an accepted standard value for bacterial cells. We have found that the dry-matter content of bacteria may be more than twice as high as generally assumed. The main reason for the low estimates seems to be that proper corrections for intercellular water have not been made when estimating the wet weight of the cells. Using three different bacterial strains, we determined a dry-matter content of cells ranging from 31 to 57%, suggesting not only that the accepted standard value is much too low but also that it is far from standard. To convert bacterial biovolume into biomass (carbon content), we suggest that 0.22 g of C cm-3 should be used as a conversion factor.

[1]  Woods,et al.  Determination of bacterial number and biomass in the marine environment , 1977, Applied and environmental microbiology.

[2]  R. Macleod,et al.  NUTRITION AND MIETABOLISMNI OF MXIARINE BACTERIA ' XIII . INTRACELLULAR CONCENTRATIONS OF SODIUM AND POTASSIUM IONS IN A MARINE PSEUDOMONAD , 2022 .

[3]  J. Clegg,et al.  METABOLISM AND THE INTRACELLULAR ENVIRONMENT: THE VICINAL–WATER NETWORK MODEL1 , 1979 .

[4]  E. Paul,et al.  Conversion of Biovolume Measurements of Soil Organisms, Grown Under Various Moisture Tensions, to Biomass and Their Nutrient Content , 1979, Applied and environmental microbiology.

[5]  I. Kuntz,et al.  The properties of water in biological systems. , 1974, Annual review of biophysics and bioengineering.

[6]  J. Fuhrman,et al.  Bacterioplankton Secondary Production Estimates for Coastal Waters of British Columbia, Antarctica, and California , 1980, Applied and environmental microbiology.

[7]  J. Strickland,et al.  Sinking rates of marine phytoplankton measured with a fluorometer , 1967 .

[8]  D. Rickwood,et al.  Buoyant densities and hydration of nucleic acids, proteins and nucleoprotein complexes in metrizamide. , 1973, Biochimica et biophysica acta.

[9]  R. Ferguson,et al.  Contribution of bacteria to standing crop of coastal plankton1 , 1976 .

[10]  J. Thompson,et al.  Functions of Na+ and K+ in the active transport of -aminoisobutyric acid in a marine pseudomonad. , 1971, The Journal of biological chemistry.

[11]  R. Macleod,et al.  Penetrability of a marine pseudomonad by inulin, sucrose, and glycerol and its relation to the mechanism of lysis. , 1970, Canadian journal of microbiology.

[12]  G. Sprott,et al.  Kinetics of Naplus-dependent amino acid transport using cells and membrane vesicles of a marine pseudomonad. , 1975, Canadian journal of microbiology.

[13]  H. E. Kubitschek,et al.  Buoyant density constancy during the cell cycle of Escherichia coli , 1983, Journal of bacteriology.

[14]  D. Kushner,et al.  Cell-bound cations of the moderately halophilic bacterium Vibrio costicola , 1977, Journal of bacteriology.

[15]  J. Clegg,et al.  Cellular and molecular consequences of reduced cell water content. , 1982, Cryobiology.

[16]  W. Hayes,et al.  Experiments in microbial genetics , 1968 .

[17]  W. Bowden.  Comparison of two direct-count techniques for enumerating aquatic bacteria , 1977, Applied and environmental microbiology.

[18]  G. Sprott,et al.  Some properties of an unidentified halophile: growth characteristics, internal salt concentration, and morphology. , 1976, Canadian Journal of Microbiology (print).

[19]  Lars R. Bakken,et al.  Buoyant Densities and Dry-Matter Contents of Microorganisms: Conversion of a Measured Biovolume into Biomass , 1983, Applied and environmental microbiology.