Bioaugmentation of Fe(0) for the remediation of chlorinated aliphatic hydrocarbons.

ABSTRACT There has been considerable interest in the use of Fe(0) for the treatment of highly chlorinated aliphatic hydrocarbons (CAHs). Because early field studies found little biological contribution to degradation, much of the recent research has focused on abiotic processes. However, previous experiments in our laboratory have demonstrated that the rate and extent of carbon tetrachloride (CT) and chloroform (CF) degradation were enhanced when Fe(0) and methanogenic enrichment or methanogenic pure cultures were incubated together. In this study, acetate- or lactate-enriched methanogenic cultures were added to columns containing a steel wool support medium. Fe(0) served as the sole exogenous electron donor. CT, tetrachloroethene (PCE), and 1,1,1,-trichlorothane (1,1,1,-TCA) were fed to columns alone and in mixtures in reduced mineral medium. Columns were operated for periods of up to 90 months. Based on extent of dechlorination, an enhancement was observed for CT and 1,1,1-TCA when methanogenic enrichme...

[1]  T. Phelps,et al.  Biogeochemical dynamics in zero-valent iron columns: Implications for permeable reactive barriers , 1999 .

[2]  Robert W. Gillham,et al.  Enhanced Degradation of Halogenated Aliphatics by Zero‐Valent Iron , 1994 .

[3]  Paige J. Novak,et al.  Enhanced Dechlorination of Carbon Tetrachloride and Chloroform in the Presence of Elemental Iron and Methanosarcina barkeri, Methanosarcina thermophila, or Methanosaeta concillii , 1998 .

[4]  Pedro J. J. Alvarez,et al.  Fe(0)-Supported Autotrophic Denitrification , 1998 .

[5]  Stephen H. Zinder,et al.  Physiological Ecology of Methanogens , 1993 .

[6]  M. D. Mikesell,et al.  Dechlorination of Chloroform by Methanosarcina Strains , 1990, Applied and environmental microbiology.

[7]  J. Schnoor,et al.  Reductive dechlorination of carbon tetrachloride with elemental iron , 1995 .

[8]  P. Alvarez,et al.  Utilization of Cathodic Hydrogen as Electron Donor for Chloroform Cometabolism by a Mixed, Methanogenic Culture , 1997 .

[9]  Paul G Tratnyek,et al.  Reductive dehalogenation of chlorinated methanes by iron metal. , 1994, Environmental science & technology.

[10]  Robert W. Gillham,et al.  Dechlorination of Trichloroethene in Aqueous Solution Using Fe0 , 1996 .

[11]  L. Daniels,et al.  Bacterial Methanogenesis and Growth from CO2 with Elemental Iron as the Sole Source of Electrons , 1987, Science.

[12]  Perry L. McCarty,et al.  Anaerobic degradation of halogenated 1- and 2-carbon organic compounds. , 1981, Environmental science & technology.

[13]  G. Parkin,et al.  Concentration Effects on Chlorinated Aliphatic Transformation Kinetics , 1996 .

[14]  Timothy L. Johnson,et al.  Kinetics of Halogenated Organic Compound Degradation by Iron Metal , 1996 .

[15]  A. L. Roberts,et al.  Reductive Elimination of Chlorinated Ethylenes by Zero-Valent Metals , 1996 .

[16]  J. Gossett,et al.  Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. , 1997, Science.

[17]  G. Parkin,et al.  Biotransformation of mixtures of chlorinated aliphatic hydrocarbons by an acetate-grown methanogenic enrichment culture , 1999 .

[18]  Ha Videla,et al.  Manual of biocorrosion , 1996 .