Basic fibroblast growth factor induces cell migration and proliferation after glia-specific gene transfer in mice.

Basic fibroblast growth factor (bFGF) is overexpressed in most high-grade human gliomas, implying that it is involved in the pathogenesis of these tumors. To assess the biological effect of inappropriate production of bFGF in normal astrocytes, we developed a system for glia-specific gene transfer in transgenic mice. A transgene encoding the receptor for subgroup A avian leukosis virus and controlled by the astrocyte-specific glial fibrillary acidic protein promoter permits efficient glia-specific transfer of genes carried by subgroup A avian leukosis virus vectors. With this system, we have demonstrated that bFGF induces proliferation and migration of glial cells in vivo, without the induction of tumors.

[1]  Paul R. Cooper,et al.  The Practice of Neurosurgery , 1998 .

[2]  M. Ebersold,et al.  The Practice of Neurosurgery , 1997 .

[3]  A. Aguzzi,et al.  Development and malignant progression of astrocytomas in GFAP-v-src transgenic mice , 1997, Oncogene.

[4]  M. Wigler,et al.  PTEN, a Putative Protein Tyrosine Phosphatase Gene Mutated in Human Brain, Breast, and Prostate Cancer , 1997, Science.

[5]  James Cd,et al.  Molecular genetics and molecular biology advances in brain tumors. , 1996 .

[6]  J. Olson,et al.  Lack of p16INK4 or retinoblastoma protein (pRb), or amplification-associated overexpression of cdk4 is observed in distinct subsets of malignant glial tumors and cell lines. , 1995, Cancer research.

[7]  S. Brem,et al.  The potential role of basic fibroblast growth factor in the transformation of cultured primary human fetal astrocytes and the proliferation of human glioma (U-87) cells. , 1995, Neurosurgery.

[8]  D. Silbergeld,et al.  Modification of human glioma locomotion in vitro by cytokines EGF, bFGF, PDGFbb, NGF, and TNF alpha. , 1995, Neurosurgery.

[9]  A. Kingsman,et al.  A transient three-plasmid expression system for the production of high titer retroviral vectors. , 1995, Nucleic acids research.

[10]  A. Yu,et al.  Astrogliosis in culture. IV. Effects of basic fibroblast growth factor , 1995, Journal of neuroscience research.

[11]  H. Varmus,et al.  A system for tissue-specific gene targeting: transgenic mice susceptible to subgroup A avian leukosis virus-based retroviral vectors. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[12]  J. Silver,et al.  Immortalization of immature and mature mouse astrocytes with SV40 T antigen , 1994, Journal of neuroscience research.

[13]  V. P. Collins,et al.  Functional characterization of an EGF receptor with a truncated extracellular domain expressed in glioblastomas with EGFR gene amplification. , 1994, Oncogene.

[14]  A. Messing,et al.  GFAP promoter directs astrocyte-specific expression in transgenic mice , 1994, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[15]  H. Varmus,et al.  A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptor , 1993, Cell.

[16]  H. Varmus,et al.  Isolation of a chicken gene that confers susceptibility to infection by subgroup A avian leukosis and sarcoma viruses , 1993, Journal of virology.

[17]  B. Shilo,et al.  breathless, a Drosophila FGF receptor homolog, is essential for migration of tracheal and specific midline glial cells. , 1992, Genes & development.

[18]  D. Littman,et al.  Packaging system for rapid production of murine leukemia virus vectors with variable tropism , 1992, Journal of virology.

[19]  H. Varmus,et al.  Transgenes expressing the Wnt-1 and int-2 proto-oncogenes cooperate during mammary carcinogenesis in doubly transgenic mice , 1992, Molecular and cellular biology.

[20]  Douglas C. Miller,et al.  Immunohistochemical localization of basic fibroblast growth factor in astrocytomas. , 1990, Cancer research.

[21]  M. Jaye,et al.  Gene expression of fibroblast growth factors in human gliomas and meningiomas: demonstration of cellular source of basic fibroblast growth factor mRNA and peptide in tumor tissues. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[22]  R. McKay,et al.  CNS stem cells express a new class of intermediate filament protein , 1990, Cell.

[23]  S. Hughes,et al.  Helper-independent retrovirus vectors with Rous-associated virus type O long terminal repeats , 1988, Journal of virology.

[24]  S. Hughes,et al.  Adaptor plasmids simplify the insertion of foreign DNA into helper-independent retroviral vectors , 1987, Journal of virology.

[25]  H. Varmus,et al.  Structure of viral DNA and RNA in mammalian cells infected with avian sarcoma virus. , 1980, Journal of molecular biology.

[26]  H. Temin,et al.  Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses. , 1970, Virology.

[27]  P. Vogt,et al.  Patterns of viral interference in the avian leukosis and sarcoma complex. , 1966, Virology.

[28]  Richard B. Richter,et al.  Pathology of Tumors of the Nervous System. , 1964 .