Reconstruction of associative protein networks connected with processes of sodium exchange regulation and sodium deposition in healthy volunteers based on urine proteome analysis

The study was conducted during the experiment with a 105-day isolation in an experimental complex. Urine samples were collected from six healthy volunteers. The physical activity, diurnal rhythm, temperature parameters, and levels of oxygen and carbon dioxide were controlled during the experiment. According to the program, food intake (electrolysis, water, calories, fat, carbohydrates, protein, vitamins, etc.) at each stage of the experiment was normalized to the body weight of each subject. All samples were analyzed using an LTQ FT MS ionic cyclotron resonance mass spectrometer with Fourier transform (Thermo) on the basis of the accurate mass and time (AMT) tag approach. Among more than 20 000 peptides, 690 proteotypical proteins were found. A total of 600 urine proteins were identified to be included in the database of healthy human urine proteins. For physiological interpretation of the proteome data, computer ANDCell and AND-Viso systems were used. Clustering of proteins and the application of these systems revealed proteins that were most closely associated with the regime of sodium intake and allowed building the network of their interactions.

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