Quantification of seven apolipoproteins in human plasma by proteotypic peptides using fast LC‐MS/MS

We investigated different sample pretreatment strategies and developed a standardized sample pretreatment protocol for absolute quantification of seven apolipoproteins (Apos) in human serum by LC‐MS/MS using proteotypic peptides and corresponding stable isotope‐labeled peptides as internal standards.

[1]  D. Hochstrasser,et al.  Quantitative Clinical Chemistry Proteomics (qCCP) using mass spectrometry: general characteristics and application , 2013, Clinical chemistry and laboratory medicine.

[2]  Jennifer N. Sutton,et al.  Rapid development of sensitive, high-throughput, quantitative and highly selective mass spectrometric targeted immunoassays for clinically important proteins in human plasma and serum. , 2013, Clinical biochemistry.

[3]  Kelly A. Jones,et al.  Assessment of two immunodepletion methods: off-target effects and variations in immunodepletion efficiency may confound plasma proteomics. , 2012, Journal of proteome research.

[4]  Joachim Thiery,et al.  LC-MS-based metabolomics in the clinical laboratory. , 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[5]  Michael E. Lassman,et al.  A rapid method for cross-species quantitation of apolipoproteins A1, B48 and B100 in plasma by ultra-performance liquid chromatography/tandem mass spectrometry. , 2012, Rapid communications in mass spectrometry : RCM.

[6]  Markus Scholz,et al.  Rationale and Design of the Leipzig (LIFE) Heart Study: Phenotyping and Cardiovascular Characteristics of Patients with Coronary Artery Disease , 2011, PloS one.

[7]  Uta Ceglarek,et al.  Fast liquid chromatography combined with mass spectrometry for the analysis of metabolites and proteins in human body fluids , 2011, Analytical and bioanalytical chemistry.

[8]  Andrew N Hoofnagle,et al.  Simultaneous quantification of apolipoprotein A-I and apolipoprotein B by liquid-chromatography-multiple- reaction-monitoring mass spectrometry. , 2010, Clinical chemistry.

[9]  Darryl B. Hardie,et al.  A quantitative study of the effects of chaotropic agents, surfactants, and solvents on the digestion efficiency of human plasma proteins by trypsin. , 2010, Journal of proteome research.

[10]  Gérard Hopfgartner,et al.  Accelerated tryptic digestion for the analysis of biopharmaceutical monoclonal antibodies in plasma by liquid chromatography with tandem mass spectrometric detection. , 2010, Journal of chromatography. A.

[11]  Monica H. Elliott,et al.  Current trends in quantitative proteomics. , 2009, Journal of mass spectrometry : JMS.

[12]  H. Santos,et al.  Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques. , 2009, Analytica chimica acta.

[13]  M. Davidson.  Apolipoprotein Measurements: Is More Widespread Use Clinically Indicated? , 2009, Clinical cardiology.

[14]  Christoph H Borchers,et al.  Multiple Reaction Monitoring-based, Multiplexed, Absolute Quantitation of 45 Proteins in Human Plasma* , 2009, Molecular & Cellular Proteomics.

[15]  Sridevi Devaraj,et al.  Apolipoprotein B and cardiovascular disease risk: position statement from the AACC Lipoproteins and Vascular Diseases Division Working Group on Best Practices. , 2009, Clinical chemistry.

[16]  Ronald J. Moore,et al.  Rapid sample processing for LC-MS-based quantitative proteomics using high intensity focused ultrasound. , 2008, Journal of proteome research.

[17]  Richard G Kay,et al.  The application of ultra-performance liquid chromatography/tandem mass spectrometry to the detection and quantitation of apolipoproteins in human serum. , 2007, Rapid communications in mass spectrometry : RCM.

[18]  Anna Bierczynska-Krzysik,et al.  Methods for samples preparation in proteomic research. , 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[19]  Daniel B. Martin,et al.  Computational prediction of proteotypic peptides for quantitative proteomics , 2007, Nature Biotechnology.

[20]  Shijuan Gao,et al.  Microwave-assisted Protein Preparation and Enzymatic Digestion in Proteomics*S , 2006, Molecular & Cellular Proteomics.

[21]  Leigh Anderson,et al.  Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays for Major Plasma Proteins* , 2006, Molecular & Cellular Proteomics.

[22]  M. Mann,et al.  Mass spectrometry–based proteomics turns quantitative , 2005, Nature chemical biology.

[23]  J. Capelo,et al.  Ultra fast trypsin digestion of proteins by high intensity focused ultrasound. , 2005, Journal of proteome research.

[24]  Nichole L. King,et al.  Human Plasma PeptideAtlas , 2005, Proteomics.

[25]  E. Verpoorte,et al.  Chemically modified, immobilized trypsin reactor with improved digestion efficiency. , 2005, Journal of proteome research.

[26]  S. Baumann,et al.  Standardized approach to proteome profiling of human serum based on magnetic bead separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. , 2005, Clinical chemistry.

[27]  Steven P Gygi,et al.  The absolute quantification strategy: a general procedure for the quantification of proteins and post-translational modifications. , 2005, Methods.

[28]  S. Gygi,et al.  Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[29]  P. Weber,et al.  Microwave‐enhanced enzyme reaction for protein mapping by mass spectrometry: A new approach to protein digestion in minutes , 2002, Protein science : a publication of the Protein Society.

[30]  V. Marks,et al.  Differential diagnosis by laboratory medicine : a quick reference for physicians , 2002 .

[31]  D. Russell,et al.  Proteolysis in mixed organic-aqueous solvent systems: applications for peptide mass mapping using mass spectrometry. , 2001, Analytical chemistry.

[32]  L. Thomas.  Clinical laboratory diagnostics : use and assessment ofclinical laboratory results , 1998 .

[33]  L L Needham,et al.  Isotope dilution--mass spectrometric quantification of specific proteins: model application with apolipoprotein A-I. , 1996, Clinical chemistry.

[34]  Joan C. Han,et al.  A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol. , 1994, Analytical biochemistry.