Orthogonally Engineering Matrix Topography and Rigidity to Regulate Multicellular Morphology

Programmable polymer substrates, which mimic the variable extracellular matrices in living systems, are used to regulate multicellular morphology, via orthogonally modulating the matrix topography and elasticity. The multicellular morphology is dependent on the competition between cell-matrix adhesion and cell-cell adhesion. Decreasing the cell-matrix adhesion provokes cytoskeleton reorganization, inhibits lamellipodial crawling, and thus enhances the leakiness of multicellular morphology.

[1]  S. Basu,et al.  A synthetic multicellular system for programmed pattern formation , 2005, Nature.

[2]  T. Hisada,et al.  Microtubules Modulate the Stiffness of Cardiomyocytes Against Shear Stress , 2005, Circulation research.

[3]  Wonjae Lee,et al.  The Design of a Heterocellular 3D Architecture and its Application to Monitoring the Behavior of Cancer Cells in Response to the Spatial Distribution of Endothelial Cells , 2012, Advanced materials.

[4]  Wei-Yu Lin,et al.  Photothermal effects of supramolecularly assembled gold nanoparticles for the targeted treatment of cancer cells. , 2010, Angewandte Chemie.

[5]  J. Spatz,et al.  Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility , 2012, Journal of Cell Science.

[6]  D. Endy.  Foundations for engineering biology , 2005, Nature.

[7]  Daniel G. Anderson,et al.  Cell-compatible, multicomponent protein arrays with subcellular feature resolution. , 2008, Small.

[8]  Christopher S. Chen,et al.  Matrix rigidity regulates a switch between TGF-β1–induced apoptosis and epithelial–mesenchymal transition , 2012, Molecular biology of the cell.

[9]  J. Vörös,et al.  Engineering the Extracellular Environment: Strategies for Building 2D and 3D Cellular Structures , 2010, Advanced materials.

[10]  K Schulten,et al.  VMD: visual molecular dynamics. , 1996, Journal of molecular graphics.

[11]  Lei Jiang,et al.  Bio-inspired soft polystyrene nanotube substrate for rapid and highly efficient breast cancer-cell capture , 2013 .

[12]  H. Berendsen,et al.  Molecular dynamics with coupling to an external bath , 1984 .

[13]  A. Baeumner,et al.  Capture and culturing of living cells on microstructured DNA substrates. , 2010, Small.

[14]  Tejal A Desai,et al.  Contractility-dependent modulation of cell proliferation and adhesion by microscale topographical cues. , 2008, Small.

[15]  Brian Bierie,et al.  Tumour microenvironment: TGFβ: the molecular Jekyll and Hyde of cancer , 2006, Nature Reviews Cancer.

[16]  Joachim P Spatz,et al.  Myoblast morphology and organization on biochemically micro-patterned hydrogel coatings under cyclic mechanical strain. , 2010, Biomaterials.

[17]  M. Maharbiz.  Synthetic multicellularity. , 2012, Trends in cell biology.

[18]  Forrest M Kievit,et al.  Cancer Nanotheranostics: Improving Imaging and Therapy by Targeted Delivery Across Biological Barriers , 2011, Advanced materials.

[19]  Wei Zhang,et al.  A Strategy for Depositing Different Types of Cells in Three Dimensions to Mimic Tubular Structures in Tissues , 2012, Advanced materials.

[20]  A. Boccaccini,et al.  Tuning of Cell–Biomaterial Anchorage for Tissue Regeneration , 2013, Advanced materials.

[21]  Kenneth M. Yamada,et al.  Physical state of the extracellular matrix regulates the structure and molecular composition of cell-matrix adhesions. , 2000, Molecular biology of the cell.

[22]  Kang Sun,et al.  Hydrophobic Interaction‐Mediated Capture and Release of Cancer Cells on Thermoresponsive Nanostructured Surfaces , 2013, Advanced materials.

[23]  S. Chien.  Mechanical and chemical regulation of endothelial cell polarity. , 2006, Circulation research.

[24]  J. Lahann,et al.  Physical aspects of cell culture substrates: topography, roughness, and elasticity. , 2012, Small.

[25]  Antonios G Mikos,et al.  Injectable Biomaterials for Regenerating Complex Craniofacial Tissues , 2009, Advanced materials.

[26]  M. Bornens,et al.  Cell shape and contractility regulate ciliogenesis in cell cycle–arrested cells , 2010, The Journal of cell biology.

[27]  Cynthia A. Reinhart-King,et al.  Tuning three-dimensional collagen matrix stiffness independently of collagen concentration modulates endothelial cell behavior. , 2013, Acta biomaterialia.

[28]  Jiandong Ding,et al.  Cell–Material Interactions Revealed Via Material Techniques of Surface Patterning , 2013, Advanced materials.

[29]  K. Yao,et al.  Molecular Characterization and Clinical Implications of Spindle Cells in Nasopharyngeal Carcinoma: A Novel Molecule-Morphology Model of Tumor Progression Proposed , 2013, PloS one.

[30]  Dongsheng Liu,et al.  A Triggered DNA Hydrogel Cover to Envelop and Release Single Cells , 2013, Advanced materials.

[31]  B. Honig,et al.  Coaction of intercellular adhesion and cortical tension specifies tissue surface tension , 2010, Proceedings of the National Academy of Sciences.

[32]  Rakesh K Jain,et al.  Mechanical compression drives cancer cells toward invasive phenotype , 2011, Proceedings of the National Academy of Sciences.

[33]  Jiashu Sun,et al.  Microfluidics for manipulating cells. , 2013, Small.

[34]  D. Burr,et al.  Effects of biomechanical stress on bones in animals. , 2002, Bone.

[35]  Jianping Fu,et al.  Integrated Micro/Nanoengineered Functional Biomaterials for Cell Mechanics and Mechanobiology: A Materials Perspective , 2014, Advanced materials.

[36]  G. Yi,et al.  The Topographic Effect of Zinc Oxide Nanoflowers on Osteoblast Growth and Osseointegration , 2010, Advanced materials.

[37]  P. Moghe,et al.  Control of hepatocyte function on collagen foams: sizing matrix pores toward selective induction of 2-D and 3-D cellular morphogenesis. , 2000, Biomaterials.

[38]  Shuyan Xu,et al.  From Plasma Sources to Nanoassembly WILEY-VCH Verlag GmbH & Co. KGaA , 2013 .

[39]  Xingyu Jiang,et al.  Precise Control of Cell Adhesion by Combination of Surface Chemistry and Soft Lithography , 2013, Advanced healthcare materials.

[40]  D. Ingber.  Tensegrity: the architectural basis of cellular mechanotransduction. , 1997, Annual review of physiology.

[41]  Robert Langer,et al.  Combinatorial library of lipidoids for in vitro DNA delivery. , 2012, Bioconjugate chemistry.

[42]  Chwee Teck Lim,et al.  Emerging modes of collective cell migration induced by geometrical constraints , 2012, Proceedings of the National Academy of Sciences.

[43]  Martin Bastmeyer,et al.  Cell behaviour on micropatterned substrata: limits of extracellular matrix geometry for spreading and adhesion , 2004, Journal of Cell Science.

[44]  Dai Fukumura,et al.  Tumor microenvironment abnormalities: Causes, consequences, and strategies to normalize , 2007, Journal of cellular biochemistry.

[45]  E. Calvo,et al.  Protein A-Mediated Multicellular Behavior in Staphylococcus aureus , 2008, Journal of bacteriology.

[46]  Gregory A Voth,et al.  Allostery of actin filaments: molecular dynamics simulations and coarse-grained analysis. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[47]  D. G. T. Strange,et al.  Extracellular-matrix tethering regulates stem-cell fate. , 2012, Nature materials.

[48]  Françoise Brochard-Wyart,et al.  Spreading dynamics and wetting transition of cellular aggregates , 2011, Proceedings of the National Academy of Sciences.

[49]  Alexandra M. Greiner,et al.  Vinculin Regulates the Recruitment and Release of Core Focal Adhesion Proteins in a Force-Dependent Manner , 2013, Current Biology.

[50]  Say Chye Joachim Loo,et al.  Biophysical responses upon the interaction of nanomaterials with cellular interfaces. , 2013, Accounts of chemical research.

[51]  M. Bachmann,et al.  Controlled Cell Adhesion on Poly(dopamine) Interfaces Photopatterned with Non‐Fouling Brushes , 2013, Advanced materials.

[52]  D. van der Spoel,et al.  GROMACS: A message-passing parallel molecular dynamics implementation , 1995 .

[53]  Arpita Mitra,et al.  Taxol allosterically alters the dynamics of the tubulin dimer and increases the flexibility of microtubules. , 2008, Biophysical journal.

[54]  F. Frischknecht,et al.  Tunable Substrates Unveil Chemical Complementation of a Genetic Cell Migration Defect , 2013, Advanced healthcare materials.

[55]  D. Tirrell,et al.  Collective Cell Migration on Artificial Extracellular Matrix Proteins Containing Full‐Length Fibronectin Domains , 2010, Advanced materials.

[56]  E. Laconi,et al.  Cancer as a disease of tissue pattern formation. , 2012, Progress in histochemistry and cytochemistry.

[57]  Kristi S Anseth,et al.  In Situ Control of Cell Substrate Microtopographies Using Photolabile Hydrogels , 2012, Small.

[58]  G. Berx,et al.  The cell-cell adhesion molecule E-cadherin , 2008, Cellular and Molecular Life Sciences.

[59]  J. Kohn,et al.  Tissue spreading on implantable substrates is a competitive outcome of cell–cell vs. cell–substratum adhesivity , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[60]  Pengcheng Zhu,et al.  Loss of TAK1 increases cell traction force in a ROS-dependent manner to drive epithelial–mesenchymal transition of cancer cells , 2013, Cell Death and Disease.

[61]  Ashok Ramasubramanian,et al.  Morphogenetic adaptation of the looping embryonic heart to altered mechanical loads , 2006, Developmental dynamics : an official publication of the American Association of Anatomists.

[62]  Gerrit Groenhof,et al.  GROMACS: Fast, flexible, and free , 2005, J. Comput. Chem..

[63]  S. Parkhurst,et al.  Drosophila embryos close epithelial wounds using a combination of cellular protrusions and an actomyosin purse string , 2012, Journal of Cell Science.

[64]  Anthony Atala,et al.  Development of a composite vascular scaffolding system that withstands physiological vascular conditions. , 2008, Biomaterials.

[65]  Audrey M. Bowen,et al.  Transfer Printing Techniques for Materials Assembly and Micro/Nanodevice Fabrication , 2012, Advanced materials.

[66]  Ulrich S Schwarz,et al.  Cell-ECM traction force modulates endogenous tension at cell–cell contacts , 2011, Proceedings of the National Academy of Sciences.

[67]  Jurriaan Huskens,et al.  Microcontact Printing: Limitations and Achievements , 2009 .

[68]  J. Muschler,et al.  Cell-matrix interactions in mammary gland development and breast cancer. , 2010, Cold Spring Harbor perspectives in biology.

[69]  R. Wells.  The role of matrix stiffness in regulating cell behavior , 2008, Hepatology.

[70]  Milan Mrksich,et al.  Geometric cues for directing the differentiation of mesenchymal stem cells , 2010, Proceedings of the National Academy of Sciences.

[71]  H. Guillou,et al.  Spatial organization of the extracellular matrix regulates cell–cell junction positioning , 2012, Proceedings of the National Academy of Sciences.

[72]  Lei Jiang,et al.  Programmable Fractal Nanostructured Interfaces for Specific Recognition and Electrochemical Release of Cancer Cells , 2013, Advanced materials.

[73]  V. Mironov,et al.  Engineering biological structures of prescribed shape using self-assembling multicellular systems. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[74]  Joachim P Spatz,et al.  Age-dependent changes in microscale stiffness and mechanoresponses of cells. , 2011, Small.

[75]  F. Chien,et al.  Exploring the formation of focal adhesions on patterned surfaces using super-resolution imaging. , 2011, Small.

[76]  M. Sheetz,et al.  Cell crawling mediates collective cell migration to close undamaged epithelial gaps , 2012, Proceedings of the National Academy of Sciences.

[77]  P. Janmey,et al.  Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion. , 2005, Cell motility and the cytoskeleton.

[78]  Qiaobing Xu,et al.  Nanostructured substrate fabricated by sectioning tendon using a microtome for tissue engineering , 2011, Nanotechnology.

[79]  Jing Li,et al.  Aptamer‐Mediated Efficient Capture and Release of T Lymphocytes on Nanostructured Surfaces , 2011, Advanced materials.

[80]  S. Sen,et al.  Matrix Elasticity Directs Stem Cell Lineage Specification , 2006, Cell.

[81]  R. Fässler,et al.  Mechanosensitivity and compositional dynamics of cell–matrix adhesions , 2013, EMBO reports.

[82]  Rachelle N. Palchesko,et al.  Development of Polydimethylsiloxane Substrates with Tunable Elastic Modulus to Study Cell Mechanobiology in Muscle and Nerve , 2012, PloS one.

[83]  Joachim P Spatz,et al.  Impact of local versus global ligand density on cellular adhesion. , 2011, Nano letters.

[84]  A. Grodzinsky,et al.  Cartilage tissue remodeling in response to mechanical forces. , 2000, Annual review of biomedical engineering.

[85]  J. Genzer,et al.  Tailoring Cell Adhesion Using Surface‐Grafted Polymer Gradient Assemblies , 2005 .

[86]  Christopher S. Chen,et al.  Cellular and multicellular form and function. , 2007, Advanced drug delivery reviews.

[87]  V. Nanjundiah,et al.  Histone deacetylases regulate multicellular development in the social amoeba Dictyostelium discoideum. , 2009, Journal of molecular biology.

[88]  M. Théry,et al.  Micropatterning as a tool to decipher cell morphogenesis and functions , 2010, Journal of Cell Science.

[89]  Lay Poh Tan,et al.  Nanoparticles strengthen intracellular tension and retard cellular migration. , 2014, Nano letters.

[90]  S. T. Quek,et al.  A power-law rheology-based finite element model for single cell deformation , 2012, Biomechanics and modeling in mechanobiology.