Compensation method for quantitative observation of multicolor fluorescence with nonlinear mapping

In the field of biology, compensation of multiple fluorescence is necessary for quantitative measurement of samples. Conventional methods depend on a linear combination model irrespective of the model's adequacy. Therefore, proper compensation has not been performed in some situations. To overcome this problem, we propose a method for performing nonlinear mapping with emphasis on the distribution of samples on a plane of which the base vectors are references of fluorescence. This paper describes an experiment with measurement of multiple fluorescence and compensation using a conventional method and our proposed method. Results show that multiple fluorescence is not always able to assume a linear combination model. Moreover, we confirmed that the presented method is independent of linearity of multiple fluorescence.