Photoelectric activity of dried, oriented layers of purple membrane from Halobacterium halobium.

Summary Dried layers of purple membranes containing bacteriorhodopsin oriented by an electric field during drying were investigated by photoelectric and spectroscopic methods. There is evidence to show that the photochemical cycle of bacteriorhodopsin in a l&w hydration state is intact not only spectroscopically but also electronically in these layers. It has been observed that due to the blue-light excitation of the bleached form an intermediate absorbing at 520 nm is enriched. Thus this form seems to occur in the inhibitory pathway of bacteriorhodopsin.

[1]  E. Racker,et al.  Light-dependent proton and rubidium translocation in membrane vesicles from Halobacterium halobium. , 1975, Biochemical and biophysical research communications.

[2]  R. Korenstein,et al.  Electric field effects in bacteriorhodopsin. , 1977, Biophysical journal.

[3]  S. Caplan,et al.  Bacteriorhodopsin‐loaded charged synthetic membranes utilization of light energy to generate electrical current , 1977, FEBS letters.

[4]  D. Oesterhelt,et al.  Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane. , 1974, Methods in enzymology.

[5]  S. Caplan,et al.  The direction of light‐induced pH changes in purple membrane suspensions Influence of pH and temperature , 1977, FEBS letters.

[6]  B. Karvaly,et al.  Incorporation of bacteriorhodopsin into a bilayer lipid membrane; a photoelectric‐spectroscopic study , 1976, FEBS letters.

[7]  B. Hess,et al.  Hydration effects on the photocycle of bacteriorhodopsin in thin layers of purple membrane , 1977, Nature.

[8]  R. Mendelsohn.  Thermal denaturation and photochemistry of bacteriorhodopsin from Halobacterium cutirubrum as monitored by resonance Raman spectroscopy. , 1976, Biochimica et biophysica acta.

[9]  B. Hess,et al.  The photochemical reaction of the 412 nm chromophore of bacteriorhodopsin , 1977, FEBS letters.

[10]  C. R. Goldschmidt,et al.  On the photocycle and light adaptation of dark-adapted bacteriorhodopsin. , 1977, Biophysical journal.

[11]  P. Ormos,et al.  Mechanism of generation and regulation of photopotential by bacteriorhodopsin in bimolecular lipid membrane. , 1978, Biochimica et biophysica acta.

[12]  W. Stoeckenius,et al.  Light energy conversion in Halobacterium halobium. , 1974, Journal of supramolecular structure.

[13]  B. Hess,et al.  Reversible photolysis of the purple complex in the purple membrane of Halobacterium halobium. , 1973, European journal of biochemistry.

[14]  W. Stoeckenius,et al.  Transient photovoltages in purple membrane multilayers. Charge displacement in bacteriorhodopsin and its photointermediates. , 1978, Biochimica et biophysica acta.

[15]  W. Stoeckenius,et al.  Bacteriorhodopsin: a light-driven proton pump in Halobacterium Halobium. , 1975, Biophysical journal.

[16]  W. A. Zisman,et al.  A NEW METHOD OF MEASURING CONTACT POTENTIAL DIFFERENCES IN METALS , 1932 .

[17]  B. Karvaly,et al.  Bacteriorhodopsin: A molecular photoelectric regulator Quenching of photovoltaic effect of bimolecular lipid membranes containing bacteriorhodopsin by blue light , 1977, FEBS Letters.

[18]  B Hess,et al.  Hydration effects on cis—trans isomerization of bacteriorhodopsin , 1977, FEBS letters.

[19]  B. Hess.  Technology of biomolecular design, with experimets on light cotrol of the photochemical cycle in Halobacterium halobium , 1976, FEBS letters.