A silk-based coating containing GREDVY peptide and heparin on Mg-Zn-Y-Nd alloy: improved corrosion resistance, hemocompatibility and endothelialization.

Magnesium (Mg) alloys have been intensively investigated as potential absorbable coronary stent materials as their use avoids risks such as late inflammation and restenosis generated by permanent metallic implants. Besides that, clinical trials on coronary stents fabricated from Mg alloys have made great progress recently. However, the over-rapid corrosion rate, magnesium corrosion-induced thrombosis formation and delayed endothelium regeneration continue to be problematic for coronary artery stent therapy. In this study, silk fibroin blended with heparin and GREDVY (Gly-Arg-Glu-Asp-Val-Tyr) peptide was immobilized on a HF-pretreated MgZnYNd alloy surface via a polydopamine layer to improve its corrosion resistance, blood compatibility and endothelialization. Standard electrochemical measurements along with the long-term immersion results indicated that the functionalized MgZnYNd alloy had preferable anti-corrosion abilities compared with the bare MgZnYNd alloy. The modified surface exhibited outstanding hemocompatibility with reduced platelet adhesion, hemolysis rate and prolonged blood coagulation time. Human umbilical vein endothelial cell (HUVEC) and vascular smooth muscle cell (VSMC) co-culture results revealed more attached HUVECs on the functionalized samples than on the MgZnYNd alloy surfaces. The excellent corrosion retardation, hemocompatibility and re-endothelialization of the multi-functional coating indicate a promising method in the field of biodegradable magnesium-based implantable cardiovascular stents.

[1]  David L Kaplan,et al.  Silk-based biomaterials. , 2003, Biomaterials.

[2]  Qiufen Tu,et al.  Mussel-inspired one-step adherent coating rich in amine groups for covalent immobilization of heparin: hemocompatibility, growth behaviors of vascular cells, and tissue response. , 2014, ACS applied materials & interfaces.

[3]  S. Goodman,et al.  Platelet shape change and cytoskeletal reorganization on polyurethaneureas. , 1989, Journal of biomedical materials research.

[4]  Yufeng Zheng,et al.  Multifunctional MgF2/Polydopamine Coating on Mg Alloy for Vascular Stent Application , 2015 .

[5]  Yangde Li,et al.  Characterization of micro-arc oxidation coating post-treated by hydrofluoric acid on biodegradable ZK60 magnesium alloy , 2013 .

[6]  Jian-ping Xu,et al.  Surface engineering of cardiovascular stent with endothelial cell selectivity for in vivo re-endothelialisation. , 2013, Biomaterials.

[7]  Min Ho Lee,et al.  Tailoring the composition of fluoride conversion coatings to achieve better corrosion protection of magnesium for biomedical applications. , 2014, Journal of materials chemistry. B.

[8]  R. Bartlett,et al.  Improved Hemocompatibility of Multilumen Catheters via Nitric Oxide (NO) Release from S-Nitroso-N-acetylpenicillamine (SNAP) Composite Filled Lumen. , 2016, ACS applied materials & interfaces.

[9]  Yufeng Zheng,et al.  A review on in vitro corrosion performance test of biodegradable metallic materials , 2013 .

[10]  T. Ciach,et al.  Endothelialization of polyurethanes: Surface silanization and immobilization of REDV peptide. , 2016, Colloids and surfaces. B, Biointerfaces.

[11]  Ke Yang,et al.  Cytocompatibility and Hemolysis of AZ31B Magnesium Alloy with Si-containing Coating , 2015 .

[12]  Yufeng Zheng,et al.  Bioinspired and Biomimetic AgNPs/Gentamicin-Embedded Silk Fibroin Coatings for Robust Antibacterial and Osteogenetic Applications. , 2017, ACS applied materials & interfaces.

[13]  G. Fu,et al.  In situ endothelialization of intravascular stents coated with an anti-CD34 antibody functionalized heparin-collagen multilayer. , 2010, Biomaterials.

[14]  Jian Wang,et al.  Immobilization of heparin/poly-(L)-lysine nanoparticles on dopamine-coated surface to create a heparin density gradient for selective direction of platelet and vascular cells behavior. , 2014, Acta biomaterialia.

[15]  Yakai Feng,et al.  Star-shaped copolymer grafted PEI and REDV as a gene carrier to improve migration of endothelial cells. , 2017, Biomaterials science.

[16]  T. S. Lakshmi,et al.  Controlled release of 2, 3 desulfated heparin exerts its anti-inflammatory activity by effectively inhibiting E-selectin. , 2010, Journal of biomedical materials research. Part A.

[17]  Mark D. Huffman,et al.  Executive Summary: Heart Disease and Stroke Statistics—2015 Update A Report From the American Heart Association , 2011, Circulation.

[18]  Won Jong Kim,et al.  NONOates--polyethylenimine hydrogel for controlled nitric oxide release and cell proliferation modulation. , 2011, Bioconjugate chemistry.

[19]  Xiaoyu Fu,et al.  Preparation and characterization of a thrombin inhibitor grafted polyethersulfone blending membrane with improved antithrombotic property , 2015 .

[20]  Zhisong Lu,et al.  In situ synthesis of silver nanoparticles uniformly distributed on polydopamine-coated silk fibers for antibacterial application. , 2015, Journal of colloid and interface science.

[21]  Hyoun‐Ee Kim,et al.  MgF2-coated porous magnesium/alumina scaffolds with improved strength, corrosion resistance, and biological performance for biomedical applications. , 2016, Materials science & engineering. C, Materials for biological applications.

[22]  Yufeng Zheng,et al.  In Vitro Corrosion and Cytocompatibility of a Microarc Oxidation Coating and Poly(L-lactic acid) Composite Coating on Mg-1Li-1Ca Alloy for Orthopedic Implants. , 2016, ACS applied materials & interfaces.

[23]  Yufeng Zheng,et al.  Study on the Mg-Li-Zn ternary alloy system with improved mechanical properties, good degradation performance and different responses to cells. , 2017, Acta biomaterialia.

[24]  Yufeng Zheng,et al.  Polydopamine-induced nanocomposite Ag/CaP coatings on the surface of titania nanotubes for antibacterial and osteointegration functions. , 2015, Journal of materials chemistry. B.

[25]  X. Tingfei,et al.  Enhanced Anti-corrosion Ability and Biocompatibility of PLGA Coatings on MgZnYNd Alloy by BTSE-APTES Pre-treatment for Cardiovascular Stent , 2016 .

[26]  Yakai Feng,et al.  Antimicrobial surfaces grafted random copolymers with REDV peptide beneficial for endothelialization. , 2015, Journal of materials chemistry. B.

[27]  Y. Wang,et al.  Tailoring of the dopamine coated surface with VEGF loaded heparin/poly-L-lysine particles for anticoagulation and accelerate in situ endothelialization. , 2015, Journal of biomedical materials research. Part A.

[28]  B. Mihailova,et al.  Blood compatibility of magnesium and its alloys. , 2015, Acta biomaterialia.

[29]  Haeshin Lee,et al.  Mussel-Inspired Surface Chemistry for Multifunctional Coatings , 2007, Science.

[30]  W. Ding,et al.  In vitro degradation behavior and biocompatibility of Mg-Nd-Zn-Zr alloy by hydrofluoric acid treatment. , 2013, Materials science & engineering. C, Materials for biological applications.

[31]  G. Mani,et al.  Vitamin-C delivery from CoCr alloy surfaces using polymer-free and polymer-based platforms for cardiovascular stent applications. , 2014, Langmuir : the ACS journal of surfaces and colloids.

[32]  N. Huang,et al.  Multifunctional coating based on EPC-specific peptide and phospholipid polymers for potential applications in cardiovascular implants fate. , 2016, Journal of materials chemistry. B.

[33]  Qiufen Tu,et al.  Mussel‐Inspired Coating of Polydopamine Directs Endothelial and Smooth Muscle Cell Fate for Re‐endothelialization of Vascular Devices , 2012, Advanced healthcare materials.

[34]  J. Rodríguez‐Cabello,et al.  Biofunctionalization of REDV elastin-like recombinamers improves endothelialization on CoCr alloy surfaces for cardiovascular applications. , 2015, Colloids and surfaces. B, Biointerfaces.

[35]  Jia Pei,et al.  Enhanced bioactivity of Mg-Nd-Zn-Zr alloy achieved with nanoscale MgF2 surface for vascular stent application. , 2015, ACS applied materials & interfaces.

[36]  M. Maitz,et al.  Multifunctional silk-heparin biomaterials for vascular tissue engineering applications. , 2014, Biomaterials.

[37]  N. Murthy,et al.  End-point immobilization of heparin on plasma-treated surface of electrospun polycarbonate-urethane vascular graft. , 2017, Acta biomaterialia.

[38]  David L Kaplan,et al.  Lyophilized silk fibroin hydrogels for the sustained local delivery of therapeutic monoclonal antibodies. , 2011, Biomaterials.

[39]  B. Fahlman,et al.  In vitro and in vivo corrosion, mechanical properties and biocompatibility evaluation of MgF2-coated Mg-Zn-Zr alloy as cancellous screws. , 2017, Materials science & engineering. C, Materials for biological applications.

[40]  G. Vunjak‐Novakovic,et al.  Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. , 2004, Journal of biomedical materials research. Part A.

[41]  David L Kaplan,et al.  Stabilization of enzymes in silk films. , 2009, Biomacromolecules.

[42]  Yufeng Zheng,et al.  Rapamycin-loaded nanoporous α-Fe2O3 as an endothelial favorable and thromboresistant coating for biodegradable drug-eluting Fe stent applications. , 2017, Journal of materials chemistry. B.

[43]  D. Kaplan,et al.  Materials fabrication from Bombyx mori silk fibroin , 2011, Nature Protocols.

[44]  Liguo Wang,et al.  The microstructure and properties of cyclic extrusion compression treated Mg-Zn-Y-Nd alloy for vascular stent application. , 2012, Journal of the mechanical behavior of biomedical materials.

[45]  Changyou Gao,et al.  Preparation of an Arg-Glu-Asp-Val Peptide Density Gradient on Hyaluronic Acid-Coated Poly(ε-caprolactone) Film and Its Influence on the Selective Adhesion and Directional Migration of Endothelial Cells. , 2016, ACS applied materials & interfaces.

[46]  Kaitlyn R. Ammann,et al.  Core-shell PVA/gelatin electrospun nanofibers promote human umbilical vein endothelial cell and smooth muscle cell proliferation and migration. , 2015, Acta biomaterialia.

[47]  Heungsoo Shin,et al.  Mussel-inspired immobilization of vascular endothelial growth factor (VEGF) for enhanced endothelialization of vascular grafts. , 2012, Biomacromolecules.

[48]  Pei Wang,et al.  Enhanced in Vitro and in Vivo Performance of Mg-Zn-Y-Nd Alloy Achieved with APTES Pretreatment for Drug-Eluting Vascular Stent Application. , 2016, ACS applied materials & interfaces.

[49]  Donghui Wang,et al.  Enhanced Corrosion Resistance and Biocompatibility of Magnesium Alloy by Mg-Al-Layered Double Hydroxide. , 2016, ACS applied materials & interfaces.

[50]  Pei Wang,et al.  A novel biodegradable and biologically functional arginine-based poly(ester urea urethane) coating for Mg-Zn-Y-Nd alloy: enhancement in corrosion resistance and biocompatibility. , 2017, Journal of materials chemistry. B.

[51]  Ryan A. Hoshi,et al.  The blood and vascular cell compatibility of heparin-modified ePTFE vascular grafts. , 2013, Biomaterials.