Evidence for acutely hypoxic cells in mouse tumours, and a possible mechanism of reoxygenation.

Abstract The proportion of viable hypoxic cells in EMT6/St/lu tumours assayed 18 and 24 hours after a dose of misonidazole (1.2 mg/g), which killed 80–90% of the tumour cells, was reduced from 20% in the controls to 7% in the misonidazole treated mice. In other experiments, the radiation cell-survival curve of the RIF-1 tumour was assayed in both intradermal and intramuscular sites under both air-breathing and acutely hypoxic conditions. The slope of the survival curve of the intramuscular tumours irradiated under air-breathing conditions was significantly steeper than that of the same tumours growing intradermally (D0 values of 187 rad vs. 357 rad), or when the tumours were irradiated in either site under acutely hypoxic conditions (D0 = 375 rad). Both of these experimental observations are examined in the light of data from other sources, notably from the radiation response of hypoxic cells in spheroids and from observations made from tumours implanted between close, parallel mica sheets (“sandwich” tum...

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