Cryptosporidium and Giardia in tropical recreational marine waters contaminated with domestic sewage: estimation of bathing-associated disease risks.

Sewage is a major contributor to pollution problems involving human pathogens in tropical coastal areas. This study investigated the occurrence of intestinal protozoan parasites (Giardia and Cryptosporidium) in tropical recreational marine waters contaminated with sewage. The potential risks of Cryptosporidium and Giardia infection from recreational water exposure were estimated from the levels of viable (oo) cysts (DIC+, DAPI+, PI-) found in near-shore swimming areas using an exponential dose response model. A Monte Carlo uncertainty analysis was performed in order to determine the probability distribution of risks. Microbial indicators of recreational water quality (enterococci, Clostridium perfringens) and genetic markers of sewage pollution (human-specific Bacteroidales marker [HF183] and Clostridium coccoides) were simultaneously evaluated in order to estimate the extent of water quality deterioration associated with human wastes. The study revealed the potential risk of parasite infections via primary contact with tropical marine waters contaminated with sewage; higher risk estimates for Giardia than for Cryptosporidium were found. Mean risks estimated by Monte Carlo were below the U.S. EPA upper bound on recreational risk of 0.036 for cryptosporidiosis and giardiasis for both children and adults. However, 95th percentile estimates for giardiasis for children exceeded the 0.036 level. Environmental surveillance of microbial pathogens is crucial in order to control and eradicate the effects that increasing anthropogenic impacts have on marine ecosystems and human health.

[1]  C. Gerba.  Chapter 5 Virus Occurrence and Survival in the Environmental Waters , 2007 .

[2]  Paul Bienfang,et al.  Environmental controls, oceanography and population dynamics of pathogens and harmful algal blooms: connecting sources to human exposure , 2008, Environmental health : a global access science source.

[3]  J. Rose,et al.  Assessment of Methods for Detection of Infectious Cryptosporidium Oocysts and Giardia Cysts in Reclaimed Effluents , 2003, Applied and Environmental Microbiology.

[4]  S. Pomponi,et al.  Oceans and human health: Emerging public health risks in the marine environment. , 2006, Marine pollution bulletin.

[5]  R. Overstreet.  Waterborne Parasitic Diseases in Ocean , 2013 .

[6]  J. Schijven,et al.  Modelling the sewage discharge and dispersion of Cryptosporidium and Giardia in surface water. , 2001, Water research.

[7]  J. Tibbetts.  Coastal cities: living on the edge. , 2002, Environmental health perspectives.

[8]  Willy Verstraete,et al.  Detection and quantification of the human-specific HF183 Bacteroides 16S rRNA genetic marker with real-time PCR for assessment of human faecal pollution in freshwater. , 2005, Environmental microbiology.

[9]  Christophe Caron,et al.  Towards the human intestinal microbiota phylogenetic core. , 2009, Environmental microbiology.

[10]  J. Messer,et al.  A Rapid, Specific Membrane Filtration Procedure for Enumeration of Enterococci in Recreational Water , 1998, Applied and Environmental Microbiology.

[11]  Hillel Shuval,et al.  Estimating the global burden of thalassogenic diseases: human infectious diseases caused by wastewater pollution of the marine environment. , 2003, Journal of water and health.

[12]  John F. Griffith,et al.  Comparison of Rapid Quantitative PCR-Based and Conventional Culture-Based Methods for Enumeration of Enterococcus spp. and Escherichia coli in Recreational Waters , 2010, Applied and Environmental Microbiology.

[13]  L. Fleming,et al.  Presence of Pathogens and Indicator Microbes at a Non-Point Source Subtropical Recreational Marine Beach , 2009, Applied and Environmental Microbiology.

[14]  K. Mena,et al.  Assessment of waterborne protozoan passage through conventional drinking water treatment process in Venezuela. , 2012, Journal of water and health.

[15]  Lora E Fleming,et al.  Indicators of ocean health and human health: developing a research and monitoring framework. , 2002, Environmental health perspectives.

[16]  A. Farnleitner,et al.  Clostridium perfringens Is Not Suitable for the Indication of Fecal Pollution from Ruminant Wildlife but Is Associated with Excreta from Nonherbivorous Animals and Human Sewage , 2013, Applied and Environmental Microbiology.

[17]  R. Gilman,et al.  Triosephosphate Isomerase Gene Characterization and Potential Zoonotic Transmission of Giardia duodenalis , 2003, Emerging infectious diseases.

[18]  D. Kay,et al.  Estimates of the severity of illnesses associated with bathing in marine recreational waters contaminated with domestic sewage. , 1998, International journal of epidemiology.

[19]  L. Robertson,et al.  Occurrence of Cryptosporidium Oocysts and Giardia Cysts in Sewage in Norway , 2006, Applied and Environmental Microbiology.

[20]  T. Graczyk,et al.  Molecular Characterization ofCryptosporidium Oocysts in Samples of Raw Surface Water and Wastewater , 2001, Applied and Environmental Microbiology.

[21]  J. Rodríguez-Díaz,et al.  Molecular Characterization of Sewage-Borne Pathogens and Detection of Sewage Markers in an Urban Stream in Caracas, Venezuela , 2010, Applied and Environmental Microbiology.

[22]  T. Systems.  Managing wastewater in coastal urban areas , 2013 .

[23]  J. Konz,et al.  Exposure factors handbook , 1989 .

[24]  J. Isaac-Renton,et al.  Identifying host sources, human health risk and indicators of Cryptosporidium and Giardia in a Canadian watershed influenced by urban and rural activities. , 2012, Journal of water and health.

[25]  Richard D Moore,et al.  Risks of recreational exposure to waterborne pathogens among persons with HIV/AIDS in Baltimore, Maryland. , 2009, American journal of public health.

[26]  Dimitra Kitsiou,et al.  Marine water quality monitoring: a review. , 2013, Marine pollution bulletin.

[27]  R. Fujioka.  Monitoring coastal marine waters for spore-forming bacteria of faecal and soil origin to determine point from non-point source pollution. , 2001, Water science and technology : a journal of the International Association on Water Pollution Research.

[28]  T. Collier,et al.  The coastal environment and human health: microbial indicators, pathogens, sentinels and reservoirs , 2008, Environmental health : a global access science source.

[29]  Huw Smith,et al.  Molecular Fingerprinting of Cryptosporidium Oocysts Isolated during Water Monitoring , 2006, Applied and Environmental Microbiology.

[30]  J. Fujimoto,et al.  Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces , 2004, Applied and Environmental Microbiology.

[31]  Orin C. Shanks,et al.  Sewage reflects the distribution of human faecal Lachnospiraceae. , 2013, Environmental microbiology.

[32]  J. Rose,et al.  Conducting the Dose–Response Assessment , 2014 .

[33]  A. Boehm,et al.  Swimmer risk of gastrointestinal illness from exposure to tropical coastal waters impacted by terrestrial dry-weather runoff. , 2011, Environmental science & technology.

[34]  T. Graczyk,et al.  Relationships among bather density, levels of human waterborne pathogens, and fecal coliform counts in marine recreational beach water , 2010, Parasitology Research.

[35]  Ian L. Pepper,et al.  Detection of Giardia and Cryptosporidium in marine waters , 1995 .

[36]  J. McLauchlin,et al.  Unravelling Cryptosporidium and Giardia epidemiology. , 2005, Trends in parasitology.

[37]  Y. Rao,et al.  The Coastal Ocean , 2008 .