Accelerating code to function: sizing up the protein production line.

High-throughput biology has been pioneered by genomics through the application of robotics to expedite DNA-sequencing projects. Advances in high-throughput protein methods are needed to drive the protein production line for high-throughput structural and functional analysis of newly discovered genes. This will require the development and application of a variety of recombinant-protein expression systems to produce the diversity of proteins from both humans and model organisms.

[1]  J. V. Moran,et al.  Initial sequencing and analysis of the human genome. , 2001, Nature.

[2]  P. Nordlund,et al.  Screening for soluble expression of recombinant proteins in a 96-well format. , 2001, Analytical biochemistry.

[3]  H. Lehrach,et al.  A human cDNA library for high-throughput protein expression screening. , 2000, Genomics.

[4]  C. Auffray,et al.  The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. , 1996, Genomics.

[5]  J. Albala,et al.  Array-based proteomics: the latest chip challenge , 2001, Expert review of molecular diagnostics.

[6]  H. Lehrach,et al.  A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library. , 1998, Nucleic acids research.

[7]  Eric Gouaux,et al.  A new protein folding screen: Application to the ligand binding domains of a glutamate and kainate receptor and to lysozyme and carbonic anhydrase , 1999, Protein science : a publication of the Protein Society.

[8]  H. Lehrach,et al.  A human cDNA expression library in yeast enriched for open reading frames. , 2001, Genome research.

[9]  H. Lehrach,et al.  A system for dual protein expression in Pichia pastoris and Escherichia coli. , 2000, Protein expression and purification.

[10]  Pingzuo Li,et al.  Glycosylated and phosphorylated proteins--expression in yeast and oocytes of Xenopus: prospects and challenges--relevance to expression of thermostable proteins. , 2001, Protein expression and purification.

[11]  M. Simmonds,et al.  Genome sequence of Yersinia pestis, the causative agent of plague , 2001, Nature.

[12]  J. Heyman,et al.  High-throughput expression of fusion proteins. , 2000, Methods in enzymology.

[13]  M. Uhlén,et al.  High-throughput protein expression of cDNA products as a tool in functional genomics. , 2000, Journal of biotechnology.

[14]  R. Stevens,et al.  Global Efforts in Structural Genomics , 2001, Science.

[15]  R. Gentz,et al.  Production and purification of novel secreted human proteins. , 1997, Gene.

[16]  S A Lesley,et al.  High-throughput proteomics: protein expression and purification in the postgenomic world. , 2001, Protein expression and purification.

[17]  Dieter Stoll,et al.  A microarray enzyme‐linked immunosorbent assay for autoimmune diagnostics , 2000, Electrophoresis.

[18]  Timothy B. Stockwell,et al.  The Sequence of the Human Genome , 2001, Science.

[19]  R. Wilson,et al.  Complete genome sequence of Salmonella enterica serovar Typhimurium LT2 , 2001, Nature.

[20]  Mark Gerstein,et al.  Structural proteomics of an archaeon , 2000, Nature Structural Biology.

[21]  J. Albala,et al.  From genes to proteins: High‐throughput expression and purification of the human proteome , 2001, Journal of cellular biochemistry.

[22]  Lucy J. Holt,et al.  The use of recombinant antibodies in proteomics. , 2000, Current opinion in biotechnology.

[23]  C. Morton,et al.  Comparison of Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Spodoptera frugiperda, and COS7 cells for recombinant gene expression , 2000, Molecular biotechnology.

[24]  R C Stevens,et al.  Design of high-throughput methods of protein production for structural biology. , 2000, Structure.

[25]  J. Widom,et al.  Producing selenomethionine-labeled proteins with a baculovirus expression vector system. , 1999, Structure.

[26]  D. Cahill,et al.  Protein and antibody arrays and their medical applications. , 2001, Journal of immunological methods.

[27]  Cheryl H. Arrowsmith,et al.  Protein production: feeding the crystallographers and NMR spectroscopists , 2000, Nature Structural Biology.

[28]  C. Giachelli,et al.  Phosphorylation of Osteopontin Is Required for Inhibition of Vascular Smooth Muscle Cell Calcification* , 2000, The Journal of Biological Chemistry.

[29]  Stephen M. Mount,et al.  The genome sequence of Drosophila melanogaster. , 2000, Science.

[30]  Clark,et al.  Refolding of recombinant proteins , 1998, Current opinion in biotechnology.

[31]  M Gerstein,et al.  Structural proteomics: prospects for high throughput sample preparation. , 2000, Progress in biophysics and molecular biology.

[32]  Andrew Smith.  Genome sequence of the nematode C-elegans: A platform for investigating biology , 1998 .