Swimming and ammonia toxicity in salmonids: the effect of sub lethal ammonia exposure on the swimming performance of coho salmon and the acute toxicity of ammonia in swimming and resting rainbow trout.

This study tested the hypothesis that swimming exacerbates ammonia toxicity in fish. Both sub-lethal and acute toxicity testing was conducted in a swim tunnel on swimming and resting coho salmon and rainbow trout, respectively. The sub lethal tests on coho salmon also considered the compartmentalization of ammonia within the fish. Coho salmon showed a significant linear decrease in U(crit) both with increasing water ammonia (0, 0.02, 0.04 and 0.08 mg per l NH3) and increasing plasma ammonia. Data collected included plasma pH and ammonia, muscle pH and ammonia and muscle membrane potential. Based on results found in these experiments it was concluded that the reduction in swimming performance was due to both metabolic challenges as well as depolarization of white muscle. Acute toxicity testing on swimming and resting rainbow trout revealed that swimming at (60% U(crit) or approximately 2.2 body lengths/s) decreased the LC50 level from 207+/-21.99 mg N per l in resting fish to 32.38+/-10.81. The LC50 for resting fish was significantly higher than that for swimming fish. The acute value set forth by the US EPA at the same pH is 36.1 mg N per l and may not protect swimming fish. In addition the effect of water hardness on ammonia toxicity was considered. It was found that increased water calcium ameliorates ammonia toxicity in fish living in high pH water.

[1]  R. Burrows.  Effects of accumulated excretory products on hatchery-reared salmonids: , 1964 .

[2]  P. Butler,et al.  Exposure of brown trout, Salmo trutta, to a sub-lethal concentration of copper in soft acidic water: effects upon muscle metabolism and membrane potential. , 2000, Aquatic toxicology.

[3]  H. Pörtner,et al.  Determination of intracellular pH and PCO2 after metabolic inhibition by fluoride and nitrilotriacetic acid. , 1990, Respiration physiology.

[4]  Day,et al.  Environmental acidity and white muscle recruitment during swimming in the brown trout (Salmo trutta) , 1996, The Journal of experimental biology.

[5]  P. W. Hochachka,et al.  2 – Metabolic and Enzyme Regulation during Rest-to-Work Transition: A Mammal versus Mollusc Comparison , 1983 .

[6]  P. W. Hochachka,et al.  Metabolic organization of liver during spawning migration of sockeye salmon. , 1983, The American journal of physiology.

[7]  G. Iwama,et al.  The Effect of Short-duration Seawater Exposure on the Swimming Performance of Wild and Hatchery-reared Juvenile Coho Salmon (Oncorhynchus kisutch) during Smoltification , 1994 .

[8]  G. J. Glova,et al.  Critical swimming speeds of coho salmon (Oncorhynchus kisutch) fry to smolt stages in relation to salinity and temperature , 1977 .

[9]  C. Wood,et al.  EFFECTS OF AMMONIA ON SURVIVAL, SWIMMING AND ACTIVITIES OF ENZYMES OF NITROGEN METABOLISM IN THE LAKE MAGADI TILAPIA OREOCHROMIS ALCALICUS GRAHAMI , 1993 .

[10]  Taylor,et al.  Plasma ammonia concentration in brown trout in soft acidic water and its relationship to decreased swimming performance , 1995, The Journal of experimental biology.

[11]  T. W. Moon,et al.  Cortisol in teleosts: dynamics, mechanisms of action, and metabolic regulation , 1999, Reviews in Fish Biology and Fisheries.

[12]  F. Jensen,et al.  Isolated and combined exposure to ammonia and nitrite in rainbow trout (Oncorhynchus mykiss) : effects on electrolyte status, blood respiratory properties and brain glutamine/glutamate concentrations , 1998 .

[13]  S. Hagiwara,et al.  Resting and spike potentials of skeletal muscle fibres of salt water elasmobranch and teleost fish , 1967, The Journal of physiology.

[14]  J. M. Wilson,et al.  The mudskipper, Periophthalmodon schlosseri, actively transports[Formula: see text] against a concentration gradient. , 1999, American journal of physiology. Regulatory, integrative and comparative physiology.

[15]  P. Butler,et al.  Exposure of brown trout, Salmo trutta, to sub-lethal copper concentrations in soft acidic water and its effect upon sustained swimming performance , 1995 .

[16]  P. Butler,et al.  The resting membrane potential of white muscle from brown trout (Salmo trutta) exposed to copper in soft, acidic water. , 2000, The Journal of experimental biology.

[17]  R. H. Peterson,et al.  Influence of Fenitrothion on Swimming Velocities of Brook Trout (Salvelinus fontinalis) , 1974 .

[18]  P. W. Hochachka,et al.  The purine nucleotide cycle as two temporally separated metabolic units: a study on trout muscle. , 1988, Metabolism: clinical and experimental.

[19]  T. Yamamoto.  Electrical and mechanical properties of the red and white muscles in the silver carp. , 1972, The Journal of experimental biology.

[20]  C. Wood,et al.  The Distribution of Ammonia and H+ Between Tissue Compartments in Lemon Sole (Parophrys Vetulus) at Rest, During Hypercapnia and Following Exercise , 1988 .

[21]  S. Hinch,et al.  Effects of Swim Speed and Activity Pattern on Success of Adult Sockeye Salmon Migration through an Area of Difficult Passage , 2000 .

[22]  T. Hidaka,et al.  Biophysical and mechanical properties of red and white muscle fibres in fish , 1969, The Journal of physiology.

[23]  P. Mensi,et al.  Ammonia toxicity mechanism in fish: studies on rainbow trout (Salmo gairdneri Rich). , 1981, Ecotoxicology and environmental safety.

[24]  P. Gehrke,et al.  A respirometer with controlled water quality and computerized data acquisition for experiments with swimming fish , 2004, Fish Physiology and Biochemistry.

[25]  T. Howard.  Swimming Performance of Juvenile Coho Salmon (Oncorhynchus kisutch) Exposed to Bleached Kraft Pulpmill Effluent , 1975 .

[26]  Jonathan M. Wilson,et al.  Inhibition of ammonia excretion and production in rainbow trout during severe alkaline exposure , 1998 .

[27]  D. Randall,et al.  Compartmental Distributions of Carbon Dioxide and Ammonia in Rainbow Trout at Rest and Following Exercise, and the Effect of Bicarbonate Infusion , 1992 .

[28]  Y. Ting.  The toxicity of ammonia. , 1950, Science.

[29]  K. Iwata.  Nitrogen metabolism in the mudskipper, Periophthalmus cantonens: Changes in free amino acids and related compounds in various tissues under conditions of ammonia loading, with special reference to its high ammonia tolerance , 1988 .

[30]  D. Randall,et al.  Ammonia distribution and excretion in fish , 1987, Fish Physiology and Biochemistry.

[31]  Koréneková,et al.  Effects of Sodium , 2022 .

[32]  P. Mcgee,et al.  ASTM standards on aquatic toxicology and hazard evaluation. , 1993 .

[33]  M. B. Knoph,et al.  Toxicity of ammonia to Atlantic salmon (Salmo salar L.) in seawater - effects on plasma osmolality, ion, ammonia, urea and glucose levels and hematologic parameters , 1996 .

[34]  J. R. Brett.  The Relation of Size to Rate of Oxygen Consumption and Sustained Swimming Speed of Sockeye Salmon (Oncorhynchus nerka) , 1965 .

[35]  C. Wood,et al.  Tissue intracellular acid-base status and the fate of lactate after exhaustive exercise in the rainbow trout. , 1986, The Journal of experimental biology.

[36]  J. Meade,et al.  Effects of Sodium and Calcium on Acute Toxicity of Un-ionized Ammonia to Atlantic Salmon and Lake Trout , 1993 .

[37]  D. Jones.  The effect of hypoxia and anaemia on the swimming performaance of rainbow trout (Salmo gairdneri). , 1971, The Journal of experimental biology.

[38]  L. Smith,et al.  Swimming Endurance and Resistance to Copper and Malathion of Bluegills Treated by Long-term Exposure to Sublethal Levels of Hydrogen Sulfide , 1972 .

[39]  D. Randall,et al.  Acute Ammonia Toxicity and Ammonia Excretion in Rainbow Trout (Salmo gairdneri) , 1979 .

[40]  H. Verdouw,et al.  Ammonia determination based on indophenol formation with sodium salicylate , 1978 .

[41]  J. M. Wilson,et al.  The mudskipper, Periophthalmodon schlosseri, actively transports NH4+ against a concentration gradient. , 1999, The American journal of physiology.

[42]  G. Heigenhauser,et al.  Integrated responses to exhaustive exercise and recovery in rainbow trout white muscle: acid-base, phosphogen, carbohydrate, lipid, ammonia, fluid volume and electrolyte metabolism. , 1994, The Journal of experimental biology.

[43]  P. Mensi,et al.  Biochemical aspects of water quality criteria: The case of ammonia pollution(°) , 1981 .

[44]  N. Heisler,et al.  Studies of Ammonia in the Rainbow Trout:Physico-chemical Parameters, Acid-Base Behaviour and Respiratory Clearance , 1983 .

[45]  G. Maloiy,et al.  Urea excretion as a strategy for survival in a fish living in a very alkaline environment , 1989, Nature.