论文信息 - Re‐Evaluation of Gonadotropin‐Releasing Hormone (GnRH) Action on Pituitary Cell Differentiation with Special Regard to its Effect on LH and TSH Cell Types

Re‐Evaluation of Gonadotropin‐Releasing Hormone (GnRH) Action on Pituitary Cell Differentiation with Special Regard to its Effect on LH and TSH Cell Types

We have previously demonstrated that TRH induces several hypophyseal cell type differentiation. In the present study, we re‐evaluated the effect of GnRH on gonadotroph differentiation, and we determined whether gonadotropin is implicated in the appearance of other pituitary cell types. These effects were compared to the influence of TRH on LH and TSH cell differentiation. Pituitary primordia of 11 day‐old rat embryos were cultured in a synthetic medium until the equivalent of term, and the LH and TSH cell differentiation was visualized using an immunohistochemical technique. The effect of GnRH on pituitary hormone cells was evaluated by measuring the percentage of immunoreactive area per section.

P. Dubois | Anne G. Héritier

[1] P. Dubois,et al. Influence of thyroliberin on the rat pituitary cell type differentiation: an in vitro study. , 1993, Endocrinology.

[2] L. Jennes. Prenatal development of gonadotropin-releasing hormone receptors in the rat anterior pituitary. , 1990, Endocrinology.

[3] G. Morel,et al. Ontogeny of hypothalamic luteinizing hormone-releasing hormone (GnRH) and pituitary GnRH receptors in fetal and neonatal rats. , 1985, Endocrinology.

[4] D. Grouselle,et al. Developmental changes of thyroliberin (TRH) in the rat brain , 1985, Neuroscience Letters.

[5] Y. Combarnous,et al. Induction of pituitary lactotrope differentiation by luteinizing hormone alpha subunit. , 1984, Science.

[6] G. Morel,et al. Influence of gonadoliberin on the differentiation of rat gonadotrophs: an in vivo and in vitro study. , 1984, Neuroendocrinology.

[7] M. Dubois,et al. Immunocytological determination of gonadotropic and thyrotropic cells in fetal rat anterior pituitary during normal development and under experimental conditions. , 1981, Neuroendocrinology.