Rapid increase in PKA activity during long‐term potentiation in the hippocampal afferent fibre system to the prefrontal cortex in vivo

The purpose of the present study was to examine whether cAMP‐dependent protein kinase (PKA) was implicated in the process of long‐term potentiation (LTP) in the hippocampal afferent fibre system to the prefrontal cortex in vivo. Using a biochemical approach, we measured PKA activity at different times after induction of LTP. We show that there is an NMDA receptor‐dependent increase in PKA activity in the prefrontal cortex, only at five minutes after LTP induction. These data demonstrate a role of PKA in the induction and not the expression of cortical LTP and suggest that if PKA is involved in the late phase of LTP, it does not appear to be a persistent activation.

[1]  M. Waxham,et al.  Neuronal Activity Increases the Phosphorylation of the Transcription Factor cAMP Response Element-binding Protein (CREB) in Rat Hippocampus and Cortex* , 1996, The Journal of Biological Chemistry.

[2]  E. Kandel,et al.  Genetic Demonstration of a Role for PKA in the Late Phase of LTP and in Hippocampus-Based Long-Term Memory , 1997, Cell.

[3]  R. Iyengar,et al.  Postsynaptic CAMP pathway gates early LTP in hippocampal CA1 region , 1995, Neuron.

[4]  T. Jay,et al.  NMDA Receptor‐dependent Long‐term Potentiation in the Hippocampal Afferent Fibre System to the Prefrontal Cortex in the Rat , 1995, The European journal of neuroscience.

[5]  J. Sweatt,et al.  Transient Activation of Cyclic AMP-dependent Protein Kinase during Hippocampal Long-term Potentiation* , 1996, The Journal of Biological Chemistry.

[6]  B. Connors,et al.  Distinct forms of short-term plasticity at excitatory synapses of hippocampus and neocortex. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[7]  B. Kemp,et al.  Protein kinase C contains a pseudosubstrate prototope in its regulatory domain. , 1987, Science.

[8]  W. Quinn,et al.  cAMP-dependent protein kinase and the disruption of learning in transgenic flies , 1991, Neuron.

[9]  Serge Laroche,et al.  Long-term potentiation in the prefrontal cortex following stimulation of the hippocampal CA1/subicular region , 1990, Neuroscience Letters.

[10]  E. Kandel,et al.  Effects of cAMP simulate a late stage of LTP in hippocampal CA1 neurons. , 1993, Science.

[11]  E. Kandel,et al.  Biochemical studies of stimulus convergence during classical conditioning in Aplysia: dual regulation of adenylate cyclase by Ca2+/calmodulin and transmitter , 1991, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[12]  R. Huganir,et al.  Characterization of Protein Kinase A and Protein Kinase C Phosphorylation of the N-Methyl-D-aspartate Receptor NR1 Subunit Using Phosphorylation Site-specific Antibodies* , 1997, The Journal of Biological Chemistry.

[13]  P. Greengard,et al.  Adenosine 3':5'-monophosphate receptor proteins in mammalian brain. , 1978, The Journal of biological chemistry.

[14]  I Izquierdo,et al.  Involvement of hippocampal cAMP/cAMP-dependent protein kinase signaling pathways in a late memory consolidation phase of aversively motivated learning in rats. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[15]  E. Kandel,et al.  Recruitment of long-lasting and protein kinase A-dependent long-term potentiation in the CA1 region of hippocampus requires repeated tetanization. , 1994, Learning & memory.

[16]  J. Sarvey,et al.  The effect of high-frequency electrical stimulation and norepinephrine on cyclic AMP levels in normal versus norepinephrine-depleted rat hippocampal slices , 1985, Brain Research.

[17]  E. Villacres,et al.  Induction of CRE-Mediated Gene Expression by Stimuli That Generate Long-Lasting LTP in Area CA1 of the Hippocampus , 1996, Neuron.

[18]  T. Bliss,et al.  A synaptic model of memory: long-term potentiation in the hippocampus , 1993, Nature.

[19]  J. Hell,et al.  Cyclic AMP-dependent Protein Kinase and Protein Kinase C Phosphorylate N-Methyl-d-aspartate Receptors at Different Sites* , 1997, The Journal of Biological Chemistry.

[20]  T. Jay,et al.  Plasticity of the hippocampal-prefrontal cortex synapses , 1996, Journal of Physiology-Paris.

[21]  M. Inagaki,et al.  A monoclonal antibody to the phosphorylated form of glial fibrillary acidic protein: application to a non-radioactive method for measuring protein kinase activities. , 1991, Biochemical and biophysical research communications.

[22]  J. Scott,et al.  Localization of A-kinase through anchoring proteins. , 1994, Molecular endocrinology.

[23]  N. Tolbert,et al.  A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. , 1978, Analytical biochemistry.

[24]  J. Sweatt,et al.  NMDA Receptor Activation Increases Cyclic AMP in Area CA1 of the Hippocampus via Calcium/Calmodulin Stimulation of Adenylyl Cyclase , 1993, Journal of neurochemistry.

[25]  E. Kandel,et al.  Protein synthesis during acquisition of long-term facilitation is needed for the persistent loss of regulatory subunits of the Aplysia cAMP-dependent protein kinase. , 1990, Proceedings of the National Academy of Sciences of the United States of America.