A novel strategy for rapid detection of bacteria in water by the combination of three-dimensional surface-enhanced Raman scattering (3D SERS) and laser induced breakdown spectroscopy (LIBS).

In this study, a novel strategy based on the combination of 3D SERS and LIBS was developed for qualitative and quantitative detection of bacteria. SERS-active Ag nanoparticles (AgNPs) were prepared by an improved in situ synthesis method, and reproducible SERS spectra of bacteria were obtained by natural evaporation of a droplet of the in situ synthesized sample. Four types of bacteria were classified via principal component analysis (PCA) and hierarchy cluster analysis (HCA). LIBS acted as a quantitative technique for bacterial detection based on the intracellular mineral cations, and bacteria could be detected in a linear range of 5 × 103-5 × 107 CFU mL-1, with recovery values ranging from 81.0% to 101.7%. The whole detection process including sample preparation and detection could be completed in approximately 30 min. With short assay time and simple operation, the proposed strategy has showed great potential for bacterial analysis.

[1]  J. Almirall,et al.  Quantitative analysis of liquids from aerosols and microdrops using laser induced breakdown spectroscopy. , 2012, Analytical chemistry.

[2]  J. Moros,et al.  Dual-Spectroscopy Platform for the Surveillance of Mars Mineralogy Using a Decisions Fusion Architecture on Simultaneous LIBS-Raman Data. , 2018, Analytical chemistry.

[3]  Reinhard Niessner,et al.  Surface-enhanced Raman scattering-based label-free microarray readout for the detection of microorganisms. , 2010, Analytical chemistry.

[4]  Wei Shen,et al.  Reliable Quantitative SERS Analysis Facilitated by Core-Shell Nanoparticles with Embedded Internal Standards. , 2015, Angewandte Chemie.

[5]  Jinhuai Liu,et al.  Three-dimensional and time-ordered surface-enhanced Raman scattering hotspot matrix. , 2014, Journal of the American Chemical Society.

[6]  S. J. Rehse,et al.  Bacterial Suspensions Deposited on Microbiological Filter Material for Rapid Laser-Induced Breakdown Spectroscopy Identification , 2016, Applied spectroscopy.

[7]  J. Hafner,et al.  Utilizing 3D SERS Active Volumes in Aligned Carbon Nanotube Scaffold Substrates , 2012, Advanced materials.

[8]  J. O. Cáceres,et al.  Identification and discrimination of bacterial strains by laser induced breakdown spectroscopy and neural networks. , 2011, Talanta.

[9]  Zhihua Wang,et al.  In-situ Measurement of Sodium and Potassium Release during Oxy-Fuel Combustion of Lignite using Laser-Induced Breakdown Spectroscopy: Effects of O-2 and CO2 Concentration , 2013 .

[10]  M. Veres,et al.  Surface enhanced Raman scattering (SERS) investigation of amorphous carbon , 2004 .

[11]  Carme Pastells,et al.  Nanoparticle-based biosensors for detection of pathogenic bacteria , 2009 .

[12]  S. Schlücker.  Surface-enhanced Raman spectroscopy: concepts and chemical applications. , 2014, Angewandte Chemie.

[13]  Longyan Chen,et al.  Label-free NIR-SERS discrimination and detection of foodborne bacteria by in situ synthesis of Ag colloids , 2015, Journal of Nanobiotechnology.

[14]  I. J. Jahn,et al.  Surface-enhanced Raman spectroscopy and microfluidic platforms: challenges, solutions and potential applications. , 2017, The Analyst.

[15]  Haibo Zhou,et al.  Label and label-free based surface-enhanced Raman scattering for pathogen bacteria detection: A review. , 2017, Biosensors & bioelectronics.

[16]  A. Kudelski.  Some aspects of SERS temporal fluctuations: analysis of the most intense spectra of hydrogenated amorphous carbon deposited on silver , 2007 .

[17]  Atanu Sengupta,et al.  Bioaerosol detection and characterization by surface-enhanced Raman spectroscopy. , 2007, Journal of colloid and interface science.

[18]  Qingyu Lin,et al.  Combined Laser-Induced Breakdown with Raman Spectroscopy: Historical Technology Development and Recent Applications , 2013 .

[19]  Yuh‐Lin Wang.  Functionalized arrays of raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood , 2012, 2012 Asia Communications and Photonics Conference (ACP).

[20]  Jian Xu,et al.  Single cell Raman spectroscopy for cell sorting and imaging. , 2012, Current opinion in biotechnology.

[21]  Peidong Yang,et al.  Anisotropic etching of silver nanoparticles for plasmonic structures capable of single-particle SERS. , 2010, Journal of the American Chemical Society.

[22]  Frantisek Svec,et al.  Planar monolithic porous polymer layers functionalized with gold nanoparticles as large-area substrates for sensitive surface-enhanced Raman scattering sensing of bacteria. , 2015, Analytica chimica acta.

[23]  Chen-Han Huang,et al.  On-line SERS detection of single bacterium using novel SERS nanoprobes and a microfluidic dielectrophoresis device. , 2014, Small.

[24]  Reinhard Niessner,et al.  SERS detection of bacteria in water by in situ coating with Ag nanoparticles. , 2014, Analytical chemistry.

[25]  Hongxing Xu,et al.  Highly Surface‐roughened “Flower‐like” Silver Nanoparticles for Extremely Sensitive Substrates of Surface‐enhanced Raman Scattering , 2009 .

[26]  Joseph Maria Kumar Irudayaraj,et al.  Silver Nanosphere SERS Probes for Sensitive Identification of Pathogens , 2010 .

[27]  C. Häse,et al.  Chemiosmotic Mechanism of Antimicrobial Activity of Ag+ in Vibrio cholerae , 2002, Antimicrobial Agents and Chemotherapy.

[28]  J. O. Cáceres,et al.  Rapid identification and discrimination of bacterial strains by laser induced breakdown spectroscopy and neural networks. , 2014, Talanta.

[29]  Keita Hara,et al.  Bactericidal Actions of a Silver Ion Solution on Escherichia coli, Studied by Energy-Filtering Transmission Electron Microscopy and Proteomic Analysis , 2005, Applied and Environmental Microbiology.

[30]  Y. Duan,et al.  Simple, fast matrix conversion and membrane separation method for ultrasensitive metal detection in aqueous samples by laser-induced breakdown spectroscopy. , 2015, Analytical chemistry.

[31]  Thawatchai Maneerung,et al.  Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing , 2008 .

[32]  Jungho Hwang,et al.  Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles. , 2007, The Science of the total environment.

[33]  S. Efrima,et al.  Understanding SERS of bacteria , 2009 .

[34]  Determination and quantification of Escherichia coli by capillary electrophoresis. , 2014, The Analyst.

[35]  Fang Qian,et al.  SERS spectroscopy and SERS imaging of Shewanella oneidensis using silver nanoparticles and nanowires. , 2011, Chemical communications.

[36]  F. Lagarde,et al.  Microbiological identification by surface-enhanced Raman spectroscopy , 2017 .

[37]  Luis M Liz-Marzán,et al.  SERS detection of small inorganic molecules and ions. , 2012, Angewandte Chemie.

[38]  S. J. Rehse,et al.  Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: the effect of mixed cultures and sample dilution on bacterial identification , 2010 .

[39]  Jianming Pan,et al.  Efficient capture, rapid killing and ultrasensitive detection of bacteria by a nano-decorated multi-functional electrode sensor. , 2018, Biosensors & bioelectronics.

[40]  Pan Ding,et al.  Portable and Reliable Surface-Enhanced Raman Scattering Silicon Chip for Signal-On Detection of Trace Trinitrotoluene Explosive in Real Systems. , 2017, Analytical chemistry.

[41]  Younan Xia,et al.  Shape-controlled synthesis of metal nanostructures: the case of silver. , 2005, Chemistry.

[42]  Zhong-Qun Tian,et al.  Surface-enhanced Raman spectroscopy: bottlenecks and future directions. , 2017, Chemical communications.

[43]  Vesa P Hytönen,et al.  Core-Shell Nanorod Columnar Array Combined with Gold Nanoplate-Nanosphere Assemblies Enable Powerful In Situ SERS Detection of Bacteria. , 2016, ACS applied materials & interfaces.

[44]  V. Yam,et al.  Controlled synthesis of monodisperse silver nanocubes in water. , 2004, Journal of the American Chemical Society.