The increase in tumor oxygenation under carbogen breathing induces a decrease in the uptake of [(18)F]-fluoro-deoxy-glucose.

We investigated the impact of oxygenation status (measured by EPR oximetry) on the uptake of (18)F-FDG (measured by PET) in two different tumor models during a carbogen breathing challenge. We observed a significant drop in (18)F-FDG uptake under carbogen breathing that suggests a rapid metabolic adaptation to the oxygen environment.

[1]  W. Oyen,et al.  Effects of hyperoxygenation on FDG-uptake in head-and-neck cancer. , 2006, Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology.

[2]  Bernard Gallez,et al.  Assessment of tumor oxygenation by electron paramagnetic resonance: principles and applications , 2004, NMR in biomedicine.

[3]  J. Bussink,et al.  Cellular uptake of PET tracers of glucose metabolism and hypoxia and their linkage , 2008, European Journal of Nuclear Medicine and Molecular Imaging.

[4]  N. Sadato,et al.  Reassessment of FDG uptake in tumor cells: high FDG uptake as a reflection of oxygen-independent glycolysis dominant energy production. , 1997, Nuclear medicine and biology.

[5]  Anne Bol,et al.  Hypoxia imaging with the nitroimidazole 18F-FAZA PET tracer: a comparison with OxyLite, EPR oximetry and 19F-MRI relaxometry. , 2012, Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology.

[6]  J. Pouysségur,et al.  Hypoxia and cancer , 2007, Journal of Molecular Medicine.

[7]  C. Ling,et al.  Hypoxia-Induced increase in FDG uptake in MCF7 cells. , 2001, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[8]  J F Gross,et al.  Analysis of the effects of oxygen supply and demand on hypoxic fraction in tumors. , 1995, Acta oncologica.

[9]  C. Ling,et al.  High 18F-FDG Uptake in Microscopic Peritoneal Tumors Requires Physiologic Hypoxia , 2010, Journal of Nuclear Medicine.

[10]  C. Van de Wiele,et al.  FDG uptake, a surrogate of tumour hypoxia? , 2008, European Journal of Nuclear Medicine and Molecular Imaging.

[11]  C Clifton Ling,et al.  Dependence of FDG uptake on tumor microenvironment. , 2005, International journal of radiation oncology, biology, physics.

[12]  V. Grégoire,et al.  In vivo colocalization of 2-nitroimidazole EF5 fluorescence intensity and electron paramagnetic resonance oximetry in mouse tumors. , 2003, Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology.

[13]  J. Bussink,et al.  Effects of nicotinamide and carbogen in different murine colon carcinomas: immunohistochemical analysis of vascular architecture and microenvironmental parameters. , 2004, International journal of radiation oncology, biology, physics.

[14]  Bernard Gallez,et al.  Carbon-centered radicals as oxygen sensors for in vivo electron paramagnetic resonance: screening for an optimal probe among commercially available charcoals , 1998, Magnetic Resonance Materials in Physics, Biology and Medicine.

[15]  Julie Sutcliffe-Goulden,et al.  Analysis of the regional uptake of radiolabeled deoxyglucose analogs in human tumor xenografts. , 2004, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[16]  R. Gillies,et al.  Why do cancers have high aerobic glycolysis? , 2004, Nature Reviews Cancer.

[17]  R J Hodgkiss,et al.  Vascular architecture and microenvironmental parameters in human squamous cell carcinoma xenografts: effects of carbogen and nicotinamide. , 1999, Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology.

[18]  P. Misson,et al.  Pharmacological modifications of the partial pressure of oxygen in murine tumors: Evaluation using in vivo EPR oximetry , 1999, Magnetic resonance in medicine.

[19]  V. Grégoire,et al.  Is (18)F-FDG a surrogate tracer to measure tumor hypoxia? Comparison with the hypoxic tracer (14)C-EF3 in animal tumor models. , 2010, Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology.

[20]  R L Wahl,et al.  Fluorodeoxyglucose uptake in human cancer cell lines is increased by hypoxia. , 1995, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[21]  A. W. Simonetti,et al.  Effect of carbogen breathing on the physiological profile of human glioma xenografts , 1999, Magnetic resonance in medicine.

[22]  Thomas Scholbach,et al.  pO polarography, contrast enhanced color duplex sonography (CDS), [18F] fluoromisonidazole and [18F] fluorodeoxyglucose positron emission tomography: validated methods for the evaluation of therapy-relevant tumor oxygenation or only bricks in the puzzle of tumor hypoxia? , 2007, BMC Cancer.

[23]  Johan Bussink,et al.  Aerobic glycolysis in cancers: Implications for the usability of oxygen‐responsive genes and fluorodeoxyglucose‐PET as markers of tissue hypoxia , 2008, International journal of cancer.

[24]  James B. Mitchell,et al.  The influence of tumor oxygenation on 18F-FDG (Fluorine-18 Deoxyglucose) uptake: A mouse study using positron emission tomography (PET) , 2006, Radiation oncology.

[25]  Julien Verrax,et al.  Targeting lactate-fueled respiration selectively kills hypoxic tumor cells in mice. , 2008, The Journal of clinical investigation.