Malachite Green Derivatives for Two‐Photon RNA Detection

The design, preparation and characterisation of a library of malachite green (MG) derivatives for two‐photon RNA labelling is described. Some of these MG derivatives exhibit an increased affinity for an MG‐aptamer, as well as improved two‐photon sensitivity when compared to the classical malachite green chloride. The underlying mechanisms and potential benefits for in vivo RNA visualisation are discussed.

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