Simultaneous determination of the free thyroxine and triiodothyronine fractions in serum.

An ‘all in the same tube’ procedure requiring disposable materials only and involving sequential resin dialysis and ultrafiltration for simultaneous determination of the free thyroxine and triiodothyronine fractions in serum is described. The method combines a 104-fold decontamination of labelled inorganic iodide with a negligible loss of labelled hormones. The results are independent of duration of ultrafiltration and of dilution of serum with its own ultrafiltrate.

[1]  K. O. Pedersen.  A systematic study of variables affecting protein binding of thyroxine and triiodothyronine in serum. , 1974, Scandinavian journal of clinical and laboratory investigation.

[2]  H. McIntosh,et al.  Estimation of serum-free thyroxine concentration by ultrafiltration. , 1972, The Journal of laboratory and clinical medicine.

[3]  R. I. Gregerman,et al.  Free thyroxine in serum by equilibrium dialysis: effects of dilution, specific ions and inhibitors of binding. , 1972, Journal of Clinical Endocrinology and Metabolism.

[4]  M. Izumi.  Simultaneous measurement of total and free triiodothyronine and thyroxine in human serum. , 1972, Endocrinologia japonica.

[5]  P. Larsen.  Salicylate-induced increases in free triiodothyronine in human serum. Evidence of inhibition of triiodothyronine binding to thyroxine-binding globulin and thyroxine-binding prealbumin. , 1972, The Journal of clinical investigation.

[6]  K. Sterling.  Free thyroxine and triiodothyronine. , 1971, CRC critical reviews in clinical laboratory sciences.

[7]  V. Pileggi,et al.  Measurement of "free" thyroxine in serum. , 1971, Clinical chemistry.

[8]  K. O. Pedersen.  Multiple small sample ultrafiltration without special apparatus. , 1971, Scandinavian journal of clinical and laboratory investigation.

[9]  R. D. Brennan,et al.  Simulation of the thyroid hormone-binding protein interactions in human plasma. , 1970, The Journal of clinical endocrinology and metabolism.

[10]  J. Herrmann,et al.  Zur methodik der bestimmung von freiem, dialysablem thyroxin im serum , 1969 .

[11]  S. Werner,et al.  Total and free triiodothyronine in human serum. , 1967, The Journal of clinical investigation.

[12]  J. Oppenheimer,et al.  Radioiodinated impurities in commercial preparations of 131-I-thyroxine and their effect on the measurement of free thyroxine in human serum by equilibrium dialysis. , 1967, The Journal of clinical endocrinology and metabolism.

[13]  J. Plager,et al.  Effect of preliminary purification of 131-I-thyroxine on the determination of free thyroxine in serum. , 1967, The Journal of clinical endocrinology and metabolism.

[14]  K. Sterling,et al.  Free thyroxine in human serum: simplified measurement with the aid of magnesium precipitation. , 1966, The Journal of clinical investigation.

[15]  L. Braverman,et al.  A new method for measuring the free thyroid hormone in human serum and an analysis of the factors that influence its concentration. , 1965, The Journal of clinical investigation.

[16]  M. Surks,et al.  DETERMINATION OF FREE THYROXINE IN HUMAN SERUM: A THEORETICAL AND EXPERIMENTAL ANALYSIS. , 1964, The Journal of clinical endocrinology and metabolism.

[17]  A. Taurog.  Spontaneous deiodination of I-131-labeled thyroxine and related iodophenols on filter paper. , 1963, Endocrinology.

[18]  T. Rosenthal.  The effect of temperature on the pH of blood and plasma in vitro. , 1948, The Journal of biological chemistry.