Predominant expression of circulating CD3+ lymphocytes bearing gamma T cell receptor in a prolonged immunodeficiency after allogeneic bone marrow transplantation.

The cell surface expression of alpha:beta heterodimer was studied using WT31 monoclonal antibody, in peripheral blood lymphocytes (PBL) from a patient who developed a prolonged immunodeficiency after allogeneic bone marrow transplantation. This patient, grafted for chronic myelogenous leukemia, received T cell depleted bone marrow from her HLA, A, B, D matched sibling. The late occurrence of opportunistic infection, led us to analyze the phenotype of patient PBL. 70% of PBL were CD3+ and 29% WT31+, indicating that the majority of CD3+ PBL did not express the alpha:beta heterodimer. Transcription of the genes encoding the alpha, beta, and gamma chains was assessed in cell lines derived from PBL, by Northern blot analysis. We showed that the CD3+ WT31- subset expressed a truncated, beta mRNA (1.0 kb) and also truncated alpha transcript (1.4 kb). To determine the CD3-associated structure on CD3+ WT31- cell line, immunoprecipitation assays were performed using monoclonal anti-CD3 and an hetero antiserum against gamma peptides. These CD3+ WT31- cells expressed a disulfide linked dimer, composed of products of gamma gene (37 kD, 40 kD) and of undefined delta chain (45 kD). Functional analyses were performed in PBL before and after sorting with WT31 and anti-CD3 antibody. These circulating CD3+ WT31- cells were unable to proliferate when triggered with anti-T3 beads and they seemed to mediate a suppressor activity on CD3+ WT31+ cells.

[1]  F. Sigaux,et al.  Expression of the T Cell Gamma Gene by a Functionally Defined Human T Cell Clone Characterization at DNA, RNA, and Cell Membrane Level , 1988, Scandinavian journal of immunology.

[2]  Mark M. Davis,et al.  A new T-cell receptor gene located within the alpha locus and expressed early in T-cell differentiation , 1987, Nature.

[3]  N. Federspiel,et al.  The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8- T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer , 1987, The Journal of experimental medicine.

[4]  J. Seidman,et al.  Characterization of an expressed CDS-associated Ti γ-chain reveals Cγ domain polymorphism , 1987, Nature.

[5]  J. Bluestone,et al.  Characterization of murine thymocytes with CDS-associated T-cell receptor structures , 1987, Nature.

[6]  J. Coligan,et al.  Differential expression of two distinct T-cell receptors during thymocyte development , 1987, Nature.

[7]  F. Faure,et al.  A γ-chain complex forms a functional receptor on cloned human lymphocytes with natural killer-like activity , 1987, Nature.

[8]  J. Seidman,et al.  Two forms of the T-cell receptor γ protein found on peripheral blood cytotoxic T lymphocytes , 1987, Nature.

[9]  J. Seidman,et al.  A T-cell receptor γ/CD3 complex found on cloned functional lymphocytes , 1987, Nature.

[10]  S. Tonegawa,et al.  Tγ protein is expressed on murine fetal thymocytes as a disulphide-linked heterodimer , 1987, Nature.

[11]  M. Lefranc,et al.  Genetic polymorphism and exon changes of the constant regions of the human T-cell rearranging gene gamma. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[12]  T. Mak,et al.  Athymic mice express a high level of functional γ-chain but greatly reduced levels of α- and β-chain T-cell receptor messages , 1986, Nature.

[13]  F. Alt,et al.  A functional T3 molecule associated with a novel heterodimer on the surface of immature human thymocytes , 1986, Nature.

[14]  L. Lanier,et al.  Presence of Ti (WT31) negative T lymphocytes in normal blood and thymus , 1986, Nature.

[15]  F. Faure,et al.  A unique T-cell receptor complex expressed on human fetal lymphocytes displaying natural-killer-like activity , 1986, Nature.

[16]  A. Weiss,et al.  Expression of T3 in association with a molecule distinct from the T-cell antigen receptor heterodimer. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[17]  B. Dupont,et al.  Clonable T lymphocytes in T cell-depleted bone marrow transplants correlate with development of graft-v-host disease. , 1986, Blood.

[18]  J. Seidman,et al.  Identification of a putative second T-cell receptor , 1986, Nature.

[19]  R. Miller,et al.  Long-lasting deficit of functional T cell precursors in human bone marrow transplant recipients revealed by limiting dilution methods. , 1986, Journal of immunology.

[20]  F. Faure,et al.  Natural killer clones derived from fetal (25 wk) blood. Probing the human T cell receptor with WT31 monoclonal antibody , 1986, The Journal of experimental medicine.

[21]  L. Hood,et al.  The molecular genetics of the T-cell antigen receptor and T-cell antigen recognition. , 1986, Annual review of immunology.

[22]  William Buhles.  Treatment of serious cytomegalovirus infections with 9-(1,3-dihydroxy-2-propoxymethyl)guanine in patients with AIDS and other immunodeficiencies. , 1986, The New England journal of medicine.

[23]  G. Gahrton,et al.  LYMPHOCYTE RESPONSES AFTER CYTOMEGALOVIRUS INFECTION IN BONE MARROW TRANSPLANT RECIPIENTS—A ONE‐YEAR FOLLOW‐UP , 1985, Transplantation.

[24]  J. Borst,et al.  Characteristics of a monoclonal antibody (WT-31) that recognizes a common epitope on the human T cell receptor for antigen. , 1985, Journal of immunology.

[25]  E. Gluckman,et al.  CLINICAL SIGNIFICANCE OF CYTOMEGALOVIRUS VIREMIA IN BONE MARROW TRANSPLANTATION , 1985, Transplantation.

[26]  E. Reinherz,et al.  Production and characterization of antibody probes directed at constant regions of the α and β subunit of the human T cell receptor , 1985, European journal of immunology.

[27]  H. Deeg,et al.  RECOVERY OF IN VIVO CELLULAR IMMUNITY AFTER HUMAN MARROW GRAFTING: Influence of Time Postgrafting and Acute Graft‐Versus‐Host Disease , 1984, Transplantation.

[28]  E. Reinherz,et al.  Antigen-like effects of monoclonal antibodies directed at receptors on human T cell clones , 1983, The Journal of experimental medicine.

[29]  M. Greaves,et al.  A one-step purification of membrane proteins using a high efficiency immunomatrix. , 1982, The Journal of biological chemistry.

[30]  R. Callard,et al.  Distinctive functional characteristics of human „T”︁ lymphocytes defined by E rosetting or a monoclonal anti‐T cell antibody , 1981, European journal of immunology.

[31]  P. Thomas,et al.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[32]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[33]  G. Ames,et al.  Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions. , 1974, The Journal of biological chemistry.