The Impact of the Storage Conditions and Type of Clearomizers on the Increase of Heavy Metal Levels in Electronic Cigarette Liquids Retailed in Romania

The growing popularity of electronic cigarettes has raised several public health concerns, including the risks associated with heavy metals exposure via e-liquids and vapors. The purpose of this study was to determine, using atomic absorption spectrometry, the concentrations of Pb, Ni, Zn, and Co in some commercially available e-liquid samples from Romania immediately after purchase and after storage in clearomizers. Lead and zinc were found in all investigated samples before storage. The initial concentrations of Pb ranged from 0.13 to 0.26 mg L−1, while Zn concentrations were between 0.04 and 0.07 mg L−1. Traces of nickel appeared in all investigated e-liquids before storage but in very small amounts (0.01–0.02 mg L−1). Co was below the detection limits. We investigated the influence of the storage period (1, 3, and 5 days), storage temperature (22 °C and 40 °C), and type of clearomizer. In most cases, the temperature rise and storage period increase were associated with higher concentrations of heavy metals. This confirms that storage conditions can affect metal transfer and suggests that the temperature of storage is another parameter that can influence this phenomenon.

[1]  V. Slavkovich,et al.  Effects of e-liquid flavor, nicotine content, and puff duration on metal emissions from electronic cigarettes. , 2021, Environmental research.

[2]  B. Blount,et al.  Analysis of Toxic Metals in Aerosols from Devices Associated with Electronic Cigarette, or Vaping, Product Use Associated Lung Injury , 2021, Toxics.

[3]  A. Cole,et al.  Factors Associated with E-Cigarette Escalation among High School Students: A Review of the Literature , 2021, International journal of environmental research and public health.

[4]  Corby K. Martin,et al.  E-cigarette device and liquid characteristics and E-cigarette dependence: A pilot study of pod-based and disposable E-cigarette users. , 2021, Addictive behaviors.

[5]  Xue-jing Shen,et al.  Determination of trace Cr, Ni, Hg, As, and Pb in the tipping paper and filters of cigarettes by monochromatic wavelength X-ray fluorescence spectrometry , 2021 .

[6]  M. Hilpert,et al.  Metal exposure and biomarker levels among e-cigarette users in Spain. , 2021, Environmental research.

[7]  V. Slavkovich,et al.  Exposure to e-cigarette aerosol over two months induces accumulation of neurotoxic metals and alteration of essential metals in mouse brain. , 2021, Environmental research.

[8]  Wenying Lu,et al.  Electronic cigarettes: modern instruments for potential toxic lung delivery and posing risk for the development of chronic disease. , 2021, The international journal of biochemistry & cell biology.

[9]  Ting Zhang,et al.  Toxicity of electronic cigarettes: A general review of the origins, health hazards, and toxicity mechanisms. , 2021, The Science of the total environment.

[10]  P. Talbot,et al.  Design features and elemental/metal analysis of the atomizers in pod-style electronic cigarettes , 2021, PloS one.

[11]  L. Valentín-Blasini,et al.  Toxic Metal-Containing Particles in Aerosols from Pod-Type Electronic Cigarettes. , 2020, Journal of analytical toxicology.

[12]  R. Wiener,et al.  Association of electronic cigarette use with lead, cadmium, barium, and antimony body burden: NHANES 2015-2016. , 2020, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[13]  Grigorios L. Kyriakopoulos,et al.  Transfer of metals in the liquids of electronic cigarettes , 2020, Inhalation toxicology.

[14]  B. Gregory,et al.  Electronic cigarette exposure disrupts blood-brain barrier integrity and promotes neuroinflammation , 2020, Brain, Behavior, and Immunity.

[15]  Tracy Barreau,et al.  Cancer and Non-Cancer Risk Concerns from Metals in Electronic Cigarette Liquids and Aerosols , 2020, International journal of environmental research and public health.

[16]  M. Hilpert,et al.  Metal/Metalloid Levels in Electronic Cigarette Liquids, Aerosols, and Human Biosamples: A Systematic Review , 2020, Environmental health perspectives.

[17]  R. Zajdel,et al.  A Summary of In Vitro and In Vivo Studies Evaluating the Impact of E-Cigarette Exposure on Living Organisms and the Environment , 2020, International journal of molecular sciences.

[18]  T. Brüning,et al.  Airway inflammation after inhalation of nano-sized zinc oxide particles in human volunteers , 2019, BMC Pulmonary Medicine.

[19]  T. Henry,et al.  Giant cell interstitial pneumonia secondary to cobalt exposure from e-cigarette use , 2019, European Respiratory Journal.

[20]  L. Valentín-Blasini,et al.  Analysis of Toxic Metals in Liquid from Electronic Cigarettes , 2019, International journal of environmental research and public health.

[21]  P. Talbot,et al.  Effects of Model, Method of Collection, and Topography on Chemical Elements and Metals in the Aerosol of Tank-Style Electronic Cigarettes , 2019, Scientific Reports.

[22]  P. Talbot,et al.  Design Features in Multiple Generations of Electronic Cigarette Atomizers , 2019, International journal of environmental research and public health.

[23]  P. Talbot,et al.  Analysis of the elements and metals in multiple generations of electronic cigarette atomizers. , 2019, Environmental research.

[24]  Ki‐Hyun Kim,et al.  The transfer characteristics of heavy metals in electronic cigarette liquid. , 2019, Environmental research.

[25]  Jae Hong Park,et al.  Characteristics of metallic nanoparticles emitted from heated Kanthal e-cigarette coils , 2019, Journal of Nanoparticle Research.

[26]  V. Slavkovich,et al.  Metal concentrations in electronic cigarette aerosol: Effect of open-system and closed-system devices and power settings. , 2019, Environmental research.

[27]  A. Hyland,et al.  Comparison of Nicotine and Toxicant Exposure in Users of Electronic Cigarettes and Combustible Cigarettes , 2018, JAMA network open.

[28]  J. Namieśnik,et al.  New approach for e-cigarette aerosol collection by an original automatic aerosol generator utilizing melt-blown non-woven fabric. , 2018, Analytica chimica acta.

[29]  Rupali Agnihotri,et al.  Health Effects of Trace Metals in Electronic Cigarette Aerosols—a Systematic Review , 2018, Biological Trace Element Research.

[30]  C. Arnold Between the Tank and the Coil: Assessing How Metals End Up in E-Cigarette Liquid and Vapor , 2018, Environmental health perspectives.

[31]  K. Farsalinos,et al.  Detection and quantitative determination of heavy metals in electronic cigarette refill liquids using Total Reflection X-ray Fluorescence Spectrometry. , 2018, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[32]  W. Goessler,et al.  Metal Concentrations in e-Cigarette Liquid and Aerosol Samples: The Contribution of Metallic Coils , 2018, Environmental health perspectives.

[33]  M. Goniewicz,et al.  Brief Report: Lead Levels in Selected Electronic Cigarettes from Canada and the United States , 2018, International journal of environmental research and public health.

[34]  P. Talbot,et al.  Elements including metals in the atomizer and aerosol of disposable electronic cigarettes and electronic hookahs , 2017, PloS one.

[35]  D. Palazzolo,et al.  Trace Metals Derived from Electronic Cigarette (ECIG) Generated Aerosol: Potential Problem of ECIG Devices That Contain Nickel , 2017, Front. Physiol..

[36]  W. Goessler,et al.  E‐cigarettes as a source of toxic and potentially carcinogenic metals , 2017, Environmental research.

[37]  C. Proctor,et al.  Chemical Composition of Aerosol from an E-Cigarette: A Quantitative Comparison with Cigarette Smoke. , 2016, Chemical research in toxicology.

[38]  M. Brinkman,et al.  Real-Time Measurement of Electronic Cigarette Aerosol Size Distribution and Metals Content Analysis. , 2016, Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco.

[39]  Shu-Hong Zhu,et al.  E-Cigarette Design Preference and Smoking Cessation: A U.S. Population Study. , 2016, American journal of preventive medicine.

[40]  A. Navas-Acien,et al.  A direct method for e-cigarette aerosol sample collection. , 2016, Environmental research.

[41]  Ki‐Hyun Kim,et al.  Review on quantitation methods for hazardous pollutants released by e-cigarette (EC) smoking , 2016 .

[42]  P. Talbot,et al.  Strategies to Reduce Tin and Other Metals in Electronic Cigarette Aerosol , 2015, PloS one.

[43]  K. Farsalinos,et al.  Are Metals Emitted from Electronic Cigarettes a Reason for Health Concern? A Risk-Assessment Analysis of Currently Available Literature , 2015, International journal of environmental research and public health.

[44]  Tianrong Cheng Chemical evaluation of electronic cigarettes , 2014, Tobacco Control.

[45]  Priscilla Callahan-Lyon,et al.  Electronic cigarettes: human health effects , 2014, Tobacco Control.

[46]  M. Apostu,et al.  Content of Heavy Metals in Tobacco of Commonly Smoked Cigarettes in Romania , 2014 .

[47]  Casrn Provisional Peer Reviewed Toxicity Values for Cobalt , 2013 .

[48]  B. Kaličanin Potentiometric Stripping Analysis of Zinc, Cadmium and Lead in Tobacco Leaves (Nicotiana Tabacum L.) and Soil Samples , 2011 .