Differentiation of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacterial Liquid Cultures by Using Peptide Nucleic Acid-Fluorescence In Situ Hybridization Probes

ABSTRACT A blinded comparison of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with routine identification methods was performed on 74 consecutively positive mycobacterial liquid cultures. All Mycobacterium tuberculosis cultures (48 of 48) and 22 of 27 (81.5%) nontuberculous cultures were correctly identified (including one mixed culture). Five isolates yielded no reaction with either probe and were identified as Mycobacterium xenopi,Mycobacterium fortuitum, or Mycobacterium flavescens.

[1]  Poonpilas Hongmanee,et al.  Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Mycobacterium Cultures , 1999, Journal of Clinical Microbiology.

[2]  A. Troesch,et al.  Mycobacterium Species Identification and Rifampin Resistance Testing with High-Density DNA Probe Arrays , 1999, Journal of Clinical Microbiology.

[3]  A. Berno,et al.  Simultaneous genotyping and species identification using hybridization pattern recognition analysis of generic Mycobacterium DNA arrays. , 1998, Genome research.

[4]  F. Drobniewski.  Is death inevitable with multiresistant TB plus HIV infection? , 1997, The Lancet.

[5]  D. Cohn.  Treatment of multidrug-resistant tuberculosis. , 1995, The Journal of hospital infection.

[6]  J. Kilburn,et al.  Rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a Mycobacterium library , 1994, Journal of clinical microbiology.

[7]  R. Shafer,et al.  Exogenous reinfection with multidrug-resistant Mycobacterium tuberculosis in patients with advanced HIV infection. , 1993, The New England journal of medicine.

[8]  L. Ackerson,et al.  Treatment of 171 patients with pulmonary tuberculosis resistant to isoniazid and rifampin. , 1993, The New England journal of medicine.

[9]  Frank,et al.  Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis , 1993, Journal of clinical microbiology.

[10]  M. Fischl,et al.  An Outbreak of Tuberculosis Caused by Multiple-Drug-resistant Tubercle Bacilli among Patients with HIV Infection , 1992, Annals of Internal Medicine.

[11]  E. Böttger,et al.  Species identification of mycobacteria using rDNA sequencing. , 1998, Methods in molecular biology.

[12]  R. Good,et al.  Current Laboratory Methods for the Diagnosis of Tuberculosis , 1994 .

[13]  M. Francis Drobniewski,et al.  Seminars in Respiratory and Critical Care Medicine , 2022 .