A novel l-amino acid ligase from bacillus licheniformis.

l-Amino acid alpha-ligase (EC 6.3.2.28) catalyzed formation of alpha-peptide bond in unprotected l-amino acids in an ATP-dependent manner. BL00235 gene in Bacillus licheniformis NBRC12200 coded as a new l-amino acid ligase. BL00235 substrate specificity was strict; only methionine or leucine was acceptable as dipeptide N-terminal residues.

[1]  K. Kino,et al.  Dipeptide synthesis by L-amino acid ligase from Ralstonia solanacearum. , 2008, Biochemical and biophysical research communications.

[2]  S. Hashimoto,et al.  Fermentative Production of l-Alanyl-l-Glutamine by a Metabolically Engineered Escherichia coli Strain Expressing l-Amino Acid α-Ligase , 2007, Applied and Environmental Microbiology.

[3]  S. Hashimoto,et al.  ywfE in Bacillus subtilis Codes for a Novel Enzyme, l-Amino Acid Ligase , 2005, Journal of bacteriology.

[4]  K. Yokozeki,et al.  A novel and efficient enzymatic method for the production of peptides from unprotected starting materials. , 2005, Journal of biotechnology.

[5]  P. Fürst,et al.  Amino-acid substrates in new bottles: implications for clinical nutrition in the 21st century. , 2000, Nutrition.

[6]  K. Nakamura,et al.  Tastes produced by peptides containing ionic groups and by related compounds. , 1996, Bioscience, biotechnology and biochemistry.

[7]  R. López-Fandiño,et al.  Biologically active peptides and enzymatic approaches to their production. , 1996, Enzyme and microbial technology.

[8]  Y. Osajima,et al.  Antihypertensive effects of angiotensin fragments in SHR. , 1995, Bioscience, biotechnology, and biochemistry.

[9]  P. Stehle,et al.  Kinetically controlled synthesis of dipeptides using ficin as biocatalyst , 1991, Biotechnology and applied biochemistry.

[10]  H. Cheung,et al.  Binding of peptide substrates and inhibitors of angiotensin-converting enzyme. Importance of the COOH-terminal dipeptide sequence. , 1980, The Journal of biological chemistry.

[11]  H. Ueda,et al.  A novel analgesic dipeptide from bovine brain is a possible Met-enkephalin releaser , 1979, Nature.

[12]  Y. Shimonishi,et al.  Studies on the Synthesis of Peptides Containing Glutamine as the C-Terminal. I. Protection of Amide-nitrogen with Xanthyl Group during Peptide Synthesis , 1962 .

[13]  AkaboriShiro,et al.  Protection of Amide-Nitrogen for Peptide Synthesis. A Novel Synthesis of Peptides Containing C-Terminal Glutamine , 1961 .