Direct imaging of fluorescent structures behind turbid layers.

We present a method to directly image fluorescent structures inside turbid media. This is based on wave-front shaping to optimize the scattered light onto a single fluorescent particle, as the optical memory effect for a scanning image of the surroundings of this particle. We show that iterating the optimization leads to the focusing on a single particle whose surroundings are subsequently scanned. In combination with a parabolic phase pattern, this method can be extended to a three dimensional imaging method inside turbid media.

[1]  I. Vellekoop,et al.  Scattered light fluorescence microscopy: imaging through turbid layers. , 2010, Optics letters.

[2]  D. Psaltis,et al.  OPTICAL PHASE CONJUGATION FOR TURBIDITY SUPPRESSION IN BIOLOGICAL SAMPLES. , 2008, Nature photonics.

[3]  D. Conkey,et al.  High-speed scattering medium characterization with application to focusing light through turbid media. , 2012, Optics express.

[4]  Zahid Yaqoob,et al.  Measurement of the time-resolved reflection matrix for enhancing light energy delivery into a scattering medium. , 2013, Physical review letters.

[5]  O. Katz,et al.  Spectral control of broadband light through random media by wavefront shaping. , 2012, Optics letters.

[6]  Yongkeun Park,et al.  Dynamic active wave plate using random nanoparticles , 2012 .

[7]  C. Aegerter,et al.  Scattered light fluorescence microscopy in three dimensions. , 2012, Optics express.

[8]  Wooyoung Jang,et al.  Complex wavefront shaping for optimal depth-selective focusing in optical coherence tomography. , 2013, Optics express.

[9]  Exploiting the time-reversal operator for adaptive optics, selective focusing and scattering pattern analysis , 2012, CLEO 2012.

[10]  Ke Si,et al.  Complex wavefront corrections for deep tissue focusing using low coherence backscattered light , 2012 .

[11]  Feng,et al.  Memory effects in propagation of optical waves through disordered media. , 1988, Physical review letters.

[12]  O. Katz,et al.  Polarization control of multiply scattered light through random media by wavefront shaping. , 2012, Optics letters.

[13]  Demetri Psaltis,et al.  Three-dimensional scanning microscopy through thin turbid media. , 2012, Optics express.

[14]  Demetri Psaltis,et al.  Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle. , 2010, Optics express.

[15]  A. Mosk,et al.  Control of light transmission through opaque scattering media in space and time. , 2010, Physical review letters.

[16]  G. Lerosey,et al.  Controlling waves in space and time for imaging and focusing in complex media , 2012, Nature Photonics.

[17]  Feng,et al.  Correlations and fluctuations of coherent wave transmission through disordered media. , 1988, Physical review letters.

[18]  S. Popoff,et al.  Measuring the transmission matrix in optics: an approach to the study and control of light propagation in disordered media. , 2009, Physical review letters.

[19]  Yingjian Wang.  Anisoplanatism in Adaptive Optics Using Synthetic Beacons , 1996 .

[20]  D. Fried.  Anisoplanatism in adaptive optics , 1982 .

[21]  Yongkeun Park,et al.  Active spectral filtering through turbid media. , 2012, Optics letters.

[22]  Meng Cui.  A high speed wavefront determination method based on spatial frequency modulations for focusing light through random scattering media. , 2011, Optics express.

[23]  Ivo Micha Vellekoop,et al.  Focusing light through living tissue , 2010, BiOS.

[24]  E. G. van Putten,et al.  Focusing light through random photonic media by binary amplitude modulation. , 2011, Optics express.

[25]  S. Gigan,et al.  Spatio-temporal focusing of an ultrafast pulse through a multiply scattering medium. , 2011, Nature communications.

[26]  J. Bertolotti,et al.  Non-invasive imaging through opaque scattering layers , 2012, Nature.

[27]  A. Mosk,et al.  Focusing coherent light through opaque strongly scattering media. , 2007, Optics letters.

[28]  E. G. van Putten,et al.  Demixing light paths inside disordered metamaterials. , 2008, Optics express.

[29]  Sylvain Gigan,et al.  Image transmission through an opaque material. , 2010, Nature communications.

[30]  O. Katz,et al.  Focusing and compression of ultrashort pulses through scattering media , 2010, 1012.0413.

[31]  Mathias Fink,et al.  Eigenmodes of the time reversal operator: a solution to selective focusing in multiple-target media , 1994 .