BIOFILM DEVELOPMENT ON STAINLESS STEEL IN MAINS WATER

Biofilm development on stainless steel in mains water has been poorly documented to date. Results are presented of the development of potable-water biofilms over 12 months on stainless-steel grades 304 and 316, used as appendages to a large building's plumbing distribution system. The viable cell counts on grade 304 pipe after 12 months averaged 2.8 × 103 cfu cm−2, compared to 3.6 × 102 cfu cm−2 on grade 316 pipe. The viable cell and total cell count on matt (2D) stainless steel remained significantly higher (p < 0.05) when compared to smooth (2B) stainless steel after 4-months and 8-months biofilm development, but at month 12 the viable cell counts on the two steel finishes were not significantly different. Total carbohydrate levels and biomass dry weight levels were slightly but not significantly higher on grade 304 than on grade 316. A mixture of biofilm bacteria attached to stainless steel were evident, including Pseudomonas spp., Methylobacterium spp., Acinetobacter spp., Corynebacterium/Arthrobacter spp. and Micrococcus spp. Inductively coupled plasma spectrophotometer analysis of biofilms showed an accumulation of metal ions in both grades 304 and 316 pipe biofilms. Molybdenum (0.04 mg litre−1) was found to be associated with biofilms isolated from grade 316 after 4 months, and 0.05 mg litre−1 was found in the biofilms after 12-months exposure to mains water. Scanning electron microscopy provided evidence of microcolony formation of rod-shaped and coccoid-shaped bacteria and diatoms. © 1998 Elsevier Science Ltd. All rights reserved

[1]  D. J. Stewart.  The sulphate-reducing bacteria, 2nd ed.: By J. R. Postgate. Pp. 208. Cambridge University Press, 1984. £20.00 ($29.50) , 1984 .

[2]  J. Costerton,et al.  Bacterial biofilms in nature and disease. , 1987, Annual review of microbiology.

[3]  H. Paradies,et al.  Copper deterioration in a water distribution system of a county hospital in Germany caused by microbially induced corrosion – I. Description of the problem , 1992 .

[4]  D. Jain.  Microbial colonization of the surface of stainless steel coupons in a deionized water system , 1995 .

[5]  K. Cooksey,et al.  Biofilms and microbial fouling , 1983 .

[6]  C. Keevil,et al.  Influence of Plumbing Materials on Biofilm Formation and Growth of Legionella pneumophila in Potable Water Systems , 1994, Applied and environmental microbiology.

[7]  I. Beech,et al.  Interactions of exopolymers produced by sulphate-reducing bacteria with metal ions , 1995 .

[8]  Ralph Mitchell,et al.  Mechanism of the Initial Events in the Sorption of Marine Bacteria to Surfaces , 1970 .

[9]  T. Elliott,et al.  Influence of surface morphology on in vitro bacterial adherence to central venous catheters. , 1994, British journal of anaesthesia.

[10]  E. Geldreich,et al.  A new medium for the enumeration and subculture of bacteria from potable water , 1985, Applied and environmental microbiology.

[11]  Y. Lévi,et al.  Biofilm accumulation in drinking water distribution systems , 1993 .

[12]  M. Hamilton,et al.  The influence of surface features on bacterial colonization and subsequent substratum chemical changes of 316L stainless steel , 1996 .

[13]  D. Allison,et al.  A staining technique for attached bacteria and its correlation to extracellular carbohydrate production , 1984 .

[14]  I. Beech,et al.  Biofilm Development on 304 and 316 Stainless Steels in a Potable Water System , 1997 .

[15]  M. Lechevallier,et al.  Examination and characterization of distribution system biofilms , 1987, Applied and environmental microbiology.

[16]  S. Percival,et al.  Physical factors influencing bacterial fouling of type 304 and 316 stainless steels , 1998 .

[17]  M. Fletcher,et al.  An Electron-microscopic Demonstration of an Acidic Polysaccharide Involved in the Adhesion of a Marine Bacterium to Solid Surfaces , 1973 .

[18]  P. Payment,et al.  Blood agar to detect virulence factors in tap water heterotrophic bacteria , 1994, Applied and environmental microbiology.

[19]  Karsten Pedersen,et al.  Biofilm development on stainless steel and PVC surfaces in drinking water , 1990 .

[20]  M. Lechevallier,et al.  Enumeration and characterization of standard plate count bacteria in chlorinated and raw water supplies , 1980, Applied and environmental microbiology.

[21]  D. Hood,et al.  Evidence for Organic Complexed Copper in Sea Water , 1967, Nature.

[22]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[23]  B H Olson,et al.  Scanning electron microscope evidence for bacterial colonization of a drinking-water distribution system , 1981, Applied and environmental microbiology.

[24]  R. Donlan,et al.  Biofilm formation on cast iron substrata in water distribution systems , 1994 .

[25]  H. M. Shalaby,et al.  A Morphological Study of Pitting Corrosion of Copper in Soft Tap Water , 1989 .