Design and Development of Photonic Biosensors for Swine Viral Diseases Detection

In this paper we introduce a field diagnostic device based on the combination of advanced bio-sensing and photonics technologies, to tackle emerging and endemic viruses causing swine epidemics, and consequently significant economic damage in farms. The device is based on the use of microring resonators fabricated in silicon nitride with CMOS compatible techniques. In the paper, the designed and fabricated photonic integrated circuit (PIC) sensors are presented and characterized, showing an optimized performance in terms of optical losses (30 dB per ring) and extinction ration for ring resonances (15 dB). Furthermore, the results of an experiment for porcine circovirus 2 (PCV2) detection by using the developed biosensors are presented. Positive detection for different virus concentrations has been obtained. The device is currently under development in the framework of the EU Commission co-funded project SWINOSTICS.

[1]  J. Segalés,et al.  Evaluation of natural porcine circovirus type 2 (PCV2) subclinical infection and seroconversion dynamics in piglets vaccinated at different ages , 2016, Veterinary Research.

[2]  A. Griol,et al.  Demonstration of slot-waveguide structures on silicon nitride / silicon oxide platform. , 2007, Optics express.

[3]  G. Stemme,et al.  A packaged optical slot-waveguide ring resonator sensor array for multiplex label-free assays in labs-on-chips. , 2010, Lab on a chip.

[4]  M. De Sario,et al.  Integrated optical sensor for environment monitoring , 2007, 2007 ICTON Mediterranean Winter Conference.

[5]  Robert Bogue,et al.  Detecting gases with light: a review of optical gas sensor technologies , 2015 .

[6]  U. Levy,et al.  Ultrathin silicon nitride microring resonator for biophotonic applications at 970 nm wavelength , 2010 .

[7]  Varsha Sharma,et al.  Optical Sensors And Their Use In Medical Field , 2016 .

[8]  R. Webster,et al.  Genetic reassortment between avian and human influenza A viruses in Italian pigs. , 1993, Virology.

[9]  Michael E. Crenshaw.  The total energy--momentum tensor for electromagnetic fields in a dielectric , 2017 .

[10]  Amir R. Ali,et al.  Bio-optical sensor for brain activity measurement based on whispering gallery modes , 2017, Microtechnologies.

[11]  T. Opriessnig,et al.  Circulation of Porcine Parvovirus Types 1 through 6 in Serum Samples Obtained from Six Commercial Polish Pig Farms , 2017, Transboundary and emerging diseases.

[12]  D. Marris-Morini,et al.  A high efficiency silicon nitride grating coupler , 2007, 2007 4th IEEE International Conference on Group IV Photonics.

[13]  Hans Sohlström,et al.  A packaged optical slot-waveguide ring resonator sensor array for multiplex assays in Labs-on-Chip , 2009 .

[14]  E. Alocilja,et al.  Photon based sensing of pathogens in food , 2002, Proceedings of IEEE Sensors.

[15]  Ali Adibi,et al.  Multiplexed detection of lectins using integrated glycan-coated microring resonators. , 2016, Biosensors & bioelectronics.

[16]  M. Agah,et al.  Paper-based capacitive mass sensor , 2011, 2011 IEEE SENSORS Proceedings.

[17]  Phillip I. Pohl,et al.  Fluorescence detection of nitrogen dioxide with perylene/PMMA thin films , 2001 .

[18]  Hans Sohlström,et al.  High quality optical microring resonators in Si3N 4/SiO2 , 2008 .

[19]  Andrew W. Poon,et al.  Silicon-Nitride-Based Integrated Optofluidic Biochemical Sensors Using a Coupled-Resonator Optical Waveguide , 2015, Front. Mater..

[20]  L.J. Guo,et al.  Optical sensors based on active microcavities , 2006, IEEE Journal of Selected Topics in Quantum Electronics.

[21]  Jeong-Yeol Yoon,et al.  Lab-on-a-Chip Pathogen Sensors for Food Safety , 2012, Sensors.

[22]  Jeremy B. Wright,et al.  High quality factor silicon nitride ring resonators for biological sensing , 2010 .

[23]  D. O’Connor,et al.  Reorganization and expansion of the nidoviral family Arteriviridae , 2015, Archives of Virology.

[24]  Bakul C. Dave,et al.  An optical temperature sensing system based on encapsulation of a dye molecule in organosilica sol–gels , 2005 .

[25]  Jaime Lloret,et al.  Development of a Conductivity Sensor for Monitoring Groundwater Resources to Optimize Water Management in Smart City Environments , 2015, Sensors.

[26]  Antonio Varriale,et al.  A Diagnostic Device for In-Situ Detection of Swine Viral Diseases: The SWINOSTICS Project , 2019, Sensors.

[27]  Chenyang Xue,et al.  Three-gas detection system with IR optical sensor based on NDIR technology , 2015 .

[28]  P. M. Krishna,et al.  Label-free optical biosensors for food and biological sensor applications , 2018, Sensors and Actuators B: Chemical.

[29]  N. Burum,et al.  Optical sensor system for vibration measuring , 2008, 2008 50th International Symposium ELMAR.

[30]  D.M. Wilson,et al.  Sensor technologies for monitoring metabolic activity in single cells-part I: optical methods , 2004, IEEE Sensors Journal.