Bacterial alginate production: an overview of its biosynthesis and potential industrial production

Alginate is a linear polysaccharide that can be used for different applications in the food and pharmaceutical industries. These polysaccharides have a chemical structure composed of subunits of (1–4)-β-d-mannuronic acid (M) and its C-5 epimer α-l-guluronic acid (G). The monomer composition and molecular weight of alginates are known to have effects on their properties. Currently, these polysaccharides are commercially extracted from seaweed but can also be produced by Azotobacter vinelandii and Pseudomonas spp. as an extracellular polymer. One strategy to produce alginates with different molecular weights and with reproducible physicochemical characteristics is through the manipulation of the culture conditions during fermentation. This mini-review provides a comparative analysis of the metabolic pathways and molecular mechanisms involved in alginate polymerization from A. vinelandii and Pseudomonas spp. Different fermentation strategies used to produce alginates at a bioreactor laboratory scale are described.

[1]  E. Galindo,et al.  Changes in alginate molecular mass distributions, broth viscosity and morphology of Azotobacter vinelandii cultured in shake flasks , 1997, Applied Microbiology and Biotechnology.

[2]  Alvaro Díaz-Barrera,et al.  Evaluation of Gene Expression and Alginate Production in Response to Oxygen Transfer in Continuous Culture of Azotobacter vinelandii , 2014, PloS one.

[3]  Liang Wu,et al.  Overexpression of alginate lyase of Pseudoalteromonas elyakovii in Escherichia coli, purification, and characterization of the recombinant alginate lyase , 2008 .

[4]  G. Soberón-Chávez,et al.  The Azotobacter vinelandii alg8 and alg44 genes are essential for alginate synthesis and can be transcribed from an algD-independent promoter. , 1997, Gene.

[5]  C. Whitfield,et al.  Biosynthesis of the Pseudomonas aeruginosa Extracellular Polysaccharides, Alginate, Pel, and Psl , 2011, Front. Microbio..

[6]  S. Valla,et al.  Bacterial alginates: biosynthesis and applications , 1997, Applied Microbiology and Biotechnology.

[7]  I. Taylor,et al.  Alginate Biosynthesis by Pseudomonas mendocina , 1983 .

[8]  A. Díaz‐Barrera,et al.  Alginate Biosynthesis in Azotobacter vinelandii: Overview of Molecular Mechanisms in Connection with the Oxygen Availability , 2016 .

[9]  R. Ramphal,et al.  Role of Pseudomonas aeruginosa mucoid exopolysaccharide in adherence to tracheal cells , 1985, Infection and immunity.

[10]  Jochen Büchs,et al.  The viscosifying power, degree of acetylation and molecular mass of the alginate produced by Azotobacter vinelandii in shake flasks are determined by the oxygen transfer rate , 2011 .

[11]  M. Franklin,et al.  Identification of algI and algJ in the Pseudomonas aeruginosa alginate biosynthetic gene cluster which are required for alginate O acetylation , 1996, Journal of bacteriology.

[12]  E. Galindo,et al.  The oxygen transfer rate influences the molecular mass of the alginate produced by Azotobacter vinelandii , 2007, Applied Microbiology and Biotechnology.

[13]  F. Figueroa,et al.  Use of lanthanides to alleviate the effects of metal ion-deficiency in Desmodesmus quadricauda (Sphaeropleales, Chlorophyta) , 2015, Front. Microbiol..

[14]  J. Müller,et al.  Alginate production by Pseudomonas mendocina in a stirred draft fermenter , 2007 .

[15]  H. Ertesvåg,et al.  Characterization of Three New Azotobacter vinelandii Alginate Lyases, One of Which Is Involved in Cyst Germination , 2009, Journal of bacteriology.

[16]  F. Clementi,et al.  Alginate production by Azotobacter vinelandii. , 1997, Critical reviews in biotechnology.

[17]  E. Galindo,et al.  The roles of oxygen and alginate-lyase in determining the molecular weight of alginate produced by Azotobacter vinelandii , 2004, Applied Microbiology and Biotechnology.

[18]  A. Díaz‐Barrera,et al.  Alginate production and alg8 gene expression by Azotobacter vinelandii in continuous cultures , 2012, Journal of Industrial Microbiology & Biotechnology.

[19]  S. Valla,et al.  Mannuronan C-5 epimerases and cellular differentiation of Azotobacter vinelandii. , 2000, Environmental microbiology.

[20]  P. Gacesa.  Bacterial alginate biosynthesis--recent progress and future prospects. , 1998, Microbiology.

[21]  Alvaro Díaz-Barrera,et al.  Production of alginate by Azotobacter vinelandii grown at two bioreactor scales under oxygen-limited conditions , 2014, Bioprocess and Biosystems Engineering.

[22]  L. Torrance,et al.  Unusual Features of Pomoviral RNA Movement , 2011, Front. Microbio..

[23]  F. Acevedo,et al.  Manipulating the molecular weight of alginate produced by Azotobacter vinelandii in continuous cultures. , 2010, Bioresource technology.

[24]  F. Han,et al.  A Novel Alginate Lyase with High Activity on Acetylated Alginate of Pseudomonas aeruginosa FRD1 from Pseudomonas sp. QD03 , 2006 .

[25]  Shufang Wang,et al.  Simultaneous production and characterization of medium-chain-length polyhydroxyalkanoates and alginate oligosaccharides by Pseudomonas mendocina NK-01 , 2011, Applied Microbiology and Biotechnology.

[26]  H. Ertesvåg,et al.  New insights into Pseudomonas fluorescens alginate biosynthesis relevant for the establishment of an efficient production process for microbial alginates. , 2017, New biotechnology.

[27]  R. Oliveira,et al.  Polymyxin B as inhibitor of LPS contamination of Schistosoma mansoni recombinant proteins in human cytokine analysis , 2007, Microbial cell factories.

[28]  E. Galindo,et al.  Influence of dissolved oxygen tension and agitation speed on alginate production and its molecular weight in cultures of Azotobacter vinelandii* , 2000, Enzyme and microbial technology.

[29]  E. Heinzle,et al.  Oxygen supply strongly influences metabolic fluxes, the production of poly(3-hydroxybutyrate) and alginate, and the degree of acetylation of alginate in Azotobacter vinelandii , 2013 .

[30]  I. Couperwhite,et al.  Effect of limiting substrate concentration, growth rate and aeration on alginate composition and production by Azotobacter vinelandii in continuous culture , 1986 .

[31]  G. Skjåk‐Braek,et al.  Monomer sequence and acetylation pattern in some bacterial alginates. , 1986, Carbohydrate research.

[32]  A. Zeng,et al.  Effect of Oxygen on Formation and Structure ofAzotobacter vinelandii Alginate and Its Role in Protecting Nitrogenase , 2000, Applied and Environmental Microbiology.

[33]  E. Galindo,et al.  Expression of alginases and alginate polymerase genes in response to oxygen, and their relationship with the alginate molecular weight in Azotobacter vinelandii. , 2013, Enzyme and microbial technology.

[34]  G. Soberón-Chávez,et al.  Role of Azotobacter vinelandii mucA andmucC Gene Products in Alginate Production , 2000, Journal of bacteriology.

[35]  Helga Ertesvåg,et al.  Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library , 2016, BMC Genomics.

[36]  S. Marshall,et al.  Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments , 2017 .

[37]  D. Mooney,et al.  Alginate: properties and biomedical applications. , 2012, Progress in polymer science.

[38]  D. Hoefer,et al.  Biotechnologically produced microbial alginate dressings show enhanced gel forming capacity compared to commercial alginate dressings of marine origin , 2015, Journal of Materials Science: Materials in Medicine.

[39]  Enrique Galindo,et al.  Oxygen transfer rate during the production of alginate by Azotobacter vinelandii under oxygen-limited and non oxygen-limited conditions , 2011, Microbial cell factories.

[40]  A. Chakrabarty,et al.  Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa. , 1991, The Journal of biological chemistry.

[41]  B. Rehm.  Bacterial polymers: biosynthesis, modifications and applications , 2010, Nature Reviews Microbiology.

[42]  C. Peña,et al.  The signaling protein MucG negatively affects the production and the molecular mass of alginate in Azotobacter vinelandii , 2017, Applied Microbiology and Biotechnology.

[43]  H. Ertesvåg.  Alginate-modifying enzymes: biological roles and biotechnological uses , 2015, Front. Microbiol..

[44]  A. Anderson,et al.  The production of alginate by Pseudomonas mendocina in batch and continuous culture , 1989 .

[45]  Carrie-Lynn Keiski,et al.  Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas fluorescens AlgK. , 2007, Acta crystallographica. Section F, Structural biology and crystallization communications.

[46]  Enrique Galindo,et al.  Reproducing shake flasks performance in stirred fermentors: production of alginates by Azotobacter vinelandii. , 2003, Journal of biotechnology.

[47]  Z. Rehman,et al.  Genetics and regulation of bacterial alginate production. , 2014, Environmental microbiology.

[48]  A. Díaz‐Barrera,et al.  Different responses in the expression of alginases, alginate polymerase and acetylation genes during alginate production by Azotobacter vinelandii under oxygen-controlled conditions , 2017, Journal of Industrial Microbiology & Biotechnology.

[49]  Z. Rehman,et al.  Microbial alginate production, modification and its applications , 2013, Microbial biotechnology.

[50]  W. Raza,et al.  Optimization and characterization of a polysaccharide produced by Pseudomonas fluorescens WR-1 and its antioxidant activity. , 2012, Carbohydrate polymers.

[51]  J. Büchs,et al.  Molecular weight and viscosifying power of alginates produced in Azotobacter vinelandii cultures in shake flasks under low power input , 2016 .

[52]  B. Rehm,et al.  In Vitro Alginate Polymerization and the Functional Role of Alg8 in Alginate Production by Pseudomonas aeruginosa , 2006, Applied and Environmental Microbiology.

[53]  E. Galindo,et al.  Role of oxygen in the polymerization and de-polymerization of alginate produced by Azotobacter vinelandii , 2015 .

[54]  Paul A Hoskisson,et al.  Continuous culture--making a comeback? , 2005, Microbiology.

[55]  D. Segura,et al.  Encystment and alkylresorcinol production by Azotobacter vinelandii strains impaired in poly-β-hydroxybutyrate synthesis , 2003, Archives of Microbiology.

[56]  E. Conti,et al.  Alginate from Pseudomonas fluorescens and P. putida: production and properties , 1994 .

[57]  Huaping Tan,et al.  Alginate-Based Biomaterials for Regenerative Medicine Applications , 2013, Materials.

[58]  B. Rehm,et al.  Alginate Polymerization and Modification Are Linked in Pseudomonas aeruginosa , 2015, mBio.

[59]  J. Oelze,et al.  Respiratory protection of nitrogenase in Azotobacter species: is a widely held hypothesis unequivocally supported by experimental evidence? , 2000, FEMS microbiology reviews.

[60]  D. Hoefer,et al.  Microbial alginate dressings show improved binding capacity for pathophysiological factors in chronic wounds compared to commercial alginate dressings of marine origin , 2017, Journal of biomaterials applications.

[61]  F. Drabløs,et al.  Isolation of Mutant Alginate Lyases with Cleavage Specificity for Di-guluronic Acid Linkages* , 2010, The Journal of Biological Chemistry.