Trends in biohydrogen production: major challenges and state-of-the-art developments

Hydrogen has shown enormous potential to be an alternative fuel of the future. Hydrogen production technology has gained much attention in the last few decades due to advantages such as its high conversion efficiency, recyclability and non-polluting nature. Over the last few decades, biological hydrogen production has shown great promise for generating large scale sustainable energy to meet ever increasing global energy demands. Various microorganisms, namely bacteria, cyanobacteria, and algae which are capable of producing hydrogen from water, solar energy, and a variety of organic substrates, are explored and studied in detail. Current biohydrogen production technologies, however, face two major challenges such as low-yield and high production cost. Advances have been made in recent years in biohydrogen research to improve the hydrogen yield through process modifications, physiological manipulations, through metabolic and genetic engineering. Recently, cell immobilization such as microbes trapping with nanoparticles within the bioreactor has shown an increase in hydrogen production. This review critically evaluated various biological hydrogen production technologies, key challenges, and recent advancements in biohydrogen research and development.

[1]  Hang-Sik Shin,et al.  Effect of gas sparging on continuous fermentative hydrogen production , 2006 .

[2]  Debabrata Das,et al.  ADVANCES IN BIOLOGICAL HYDROGEN PRODUCTION PROCESSES , 2008 .

[3]  S. Morita,et al.  Application of rice rhizosphere microflora for hydrogen production from apple pomace , 2010 .

[4]  René H. Wijffels,et al.  Photobiological hydrogen production: photochemical e)ciency and bioreactor design , 2002 .

[5]  V. Ferreira-Leitão,et al.  Pentoses, hexoses and glycerin as substrates for biohydrogen production: An approach for Brazilian biofuel integration , 2013 .

[6]  E. Trably,et al.  Innovative CO2 pretreatment for enhancing biohydrogen production from the organic fraction of municipal solid waste (OFMSW) , 2012 .

[7]  D. Das,et al.  Photofermentative hydrogen production using purple non-sulfur bacteria Rhodobacter sphaeroides O.U.001 in an annular photobioreactor: A case study , 2009 .

[8]  G. Ananyev,et al.  Enhancing biological hydrogen production from cyanobacteria by removal of excreted products. , 2012, Journal of biotechnology.

[9]  O. Koksharova,et al.  Genetic tools for cyanobacteria , 2001, Applied Microbiology and Biotechnology.

[10]  Patrick C. Hallenbeck,et al.  Fermentative hydrogen production: Principles, progress, and prognosis , 2009 .

[11]  Dipankar Ghosh,et al.  Strategies for improving biological hydrogen production. , 2012, Bioresource technology.

[12]  Haroon S. Kheshgi,et al.  The Photobiological Production of Hydrogen: Potential Efficiency and Effectiveness as a Renewable Fuel , 2005, Critical reviews in microbiology.

[13]  Mi‐Sun Kim,et al.  Effect of carbon and nitrogen sources on photo-fermentative H2 production associated with nitrogenase, uptake hydrogenase activity, and PHB accumulation in Rhodobacter sphaeroides KD131. , 2012, Bioresource technology.

[14]  S. Dunn Hydrogen Futures: Toward a Sustainable Energy System , 2001 .

[15]  Debabrata Das,et al.  Improvement of fermentative hydrogen production: various approaches , 2004, Applied Microbiology and Biotechnology.

[16]  Amit Kumar,et al.  Development of net energy ratio and emission factor for biohydrogen production pathways. , 2011, Bioresource technology.

[17]  Gerasimos Lyberatos,et al.  Biohydrogen Production from Biomass and Wastes via Dark Fermentation: A Review , 2010 .

[18]  Olaf Kruse,et al.  Perspectives and advances of biological H2 production in microorganisms , 2006, Applied Microbiology and Biotechnology.

[19]  Elsayed Elbeshbishy,et al.  Optimization of biological hydrogen production for anaerobic co-digestion of food waste and wastewater biosolids. , 2013, Bioresource technology.

[20]  J. Armor,et al.  The multiple roles for catalysis in the production of H2 , 1999 .

[21]  P. L. Rogers,et al.  Application of Biotechnology to Industrial Sustainability , 2005 .

[22]  Ku-Fan Chen,et al.  Renewable hydrogen generation by bimetallic zero valent iron nanoparticles , 2011 .

[23]  Xiaobing Wu,et al.  Enhanced H2 gas production from bagasse using adhE inactivated Klebsiella oxytoca HP1 by sequential dark-photo fermentations. , 2010, Bioresource technology.

[24]  I. I. Ivanov,et al.  Accumulation of Ferrous Iron in Chlamydomonas reinhardtii. Influence of CO2 and Anaerobic Induction of the Reversible Hydrogenase1 , 2003, Plant Physiology.

[25]  I. Eroglu,et al.  Biohydrogen production from beet molasses by sequential dark and photofermentation , 2010 .

[26]  H. Yokoi,et al.  Simultaneous production of hydrogen and bioflocculant by Enterobacter sp. BY-29 , 2001 .

[27]  A. Tawfik,et al.  Factors affecting hydrogen production from rice straw wastes in a mesophillic up-flow anaerobic staged reactor , 2013 .

[28]  I. Eroglu,et al.  Hydrogen production by Rhodobacter sphaeroides O.U.001 in a flat plate solar bioreactor , 2008 .

[29]  Chyi-How Lay,et al.  Effects of carbonate and phosphate concentrations on hydrogen production using anaerobic sewage sludge microflora , 2004 .

[30]  Lakhveer Singh,et al.  Application of Immobilized Upflow Anaerobic Sludge Blanket Reactor Using Clostridium LS2 For Enhanced Biohydrogen Production and Treatment Efficiency of Palm Oil Mill Effluent , 2013 .

[31]  B. Genty,et al.  Control of Hydrogen Photoproduction by the Proton Gradient Generated by Cyclic Electron Flow in Chlamydomonas reinhardtii[W] , 2011, Plant Cell.

[32]  Qiang Liao,et al.  Characteristics of hydrogen production and substrate consumption of Rhodopseudomonas palustris CQK 01 in an immobilized-cell photobioreactor. , 2010, Bioresource technology.

[33]  D. Das,et al.  Microbial Biohydrogen Production by Rhodobacter sphaeroides O.U.001 in Photobioreactor , 2007 .

[34]  M. Ghirardi,et al.  Microalgae: a green source of renewable H(2). , 2000, Trends in biotechnology.

[35]  V C Kalia,et al.  Increased H2 production by immobilized microorganisms , 1995, World journal of microbiology & biotechnology.

[36]  Ballal As,et al.  Upflow anaerobic sludge blanket reactor--a review. , 2001, Indian journal of environmental health.

[37]  Ralph L. House,et al.  Chemical approaches to artificial photosynthesis , 2012, Proceedings of the National Academy of Sciences.

[38]  Debabrata Das,et al.  Hydrogen production by biological processes: a survey of literature , 2001 .

[39]  Lynne E. Macaskie,et al.  Integrating dark and light bio-hydrogen production strategies: towards the hydrogen economy , 2009 .

[40]  Venkataramana Gadhamshetty,et al.  Fermentative biohydrogen production: Evaluation of net energy gain , 2010 .

[41]  Judith Gurney BP Statistical Review of World Energy , 1985 .

[42]  J. Benemann,et al.  Hydrogen Generation Through Indirect Biophotolysis in Batch Cultures of the Nonheterocystous Nitrogen-Fixing Cyanobacterium Plectonema boryanum , 2010, Applied biochemistry and biotechnology.

[43]  Biohydrogen production using corn stalk employing Bacillus licheniformis MSU AGM 2 strain. , 2013 .

[44]  S. Oncel,et al.  Microalgal biohydrogen production considering light energy and mixing time as the two key features for scale-up. , 2012, Bioresource technology.

[45]  A. Friedl,et al.  Fermentative Hydrogen Production: Influence of Application of Mesophilic and Thermophilic Bacteria on Mass and Energy Balances , 2011 .

[46]  N. Muradov,et al.  From hydrocarbon to hydrogen–carbon to hydrogen economy , 2005 .

[47]  Sławomir Śmiech,et al.  Fossil fuel prices, exchange rate, and stock market: A dynamic causality analysis on the European market , 2013 .

[48]  R. Nandi,et al.  Microbial production of hydrogen: an overview. , 1998, Critical reviews in microbiology.

[49]  Baikun Li,et al.  Biohydrogen production from molasses by anaerobic fermentation with a pilot-scale bioreactor system , 2006 .

[50]  You-Kwan Oh,et al.  Fermentative biohydrogen production by a new chemoheterotrophic bacterium Citrobacter sp. Y19 , 2003 .

[51]  S. Polasky,et al.  Environmental, economic, and energetic costs and benefits of biodiesel and ethanol biofuels. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[52]  Hisatomo Fukui,et al.  Operation of a two-stage fermentation process producing hydrogen and methane from organic waste. , 2007, Environmental science & technology.

[53]  Jean-Philippe Steyer,et al.  Hydrogen production from agricultural waste by dark fermentation: A review , 2010 .

[54]  Chiu-Yue Lin,et al.  Biohydrogen production from soluble condensed molasses fermentation using anaerobic fermentation , 2010 .

[55]  John R. Benemann,et al.  Feasibility analysis of photobiological hydrogen production , 1997 .

[56]  R. Schulz,et al.  The [NiFe]-hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 works bidirectionally with a bias to H2 production. , 2011, Journal of the American Chemical Society.

[57]  Bruno Fabiano,et al.  Thermodynamic study and optimization of hydrogen production by Enterobacter aerogenes , 2002 .

[58]  Abdul Rahman Mohamed,et al.  Biohydrogen production in a continuous stirred tank bioreactor from synthesis gas by anaerobic photosynthetic bacterium: Rhodopirillum rubrum. , 2008, Bioresource technology.

[59]  J. Lay,et al.  Feasibility of biological hydrogen production from organic fraction of municipal solid waste , 1999 .

[60]  P. Takahashi,et al.  Biophotolysis-based Hydrogen Production by Cyanobacteria and Green Microalgae , 2007 .

[61]  R. Banerjee,et al.  Comparison of biohydrogen production processes , 2008 .

[62]  Regina Vasconcellos Antônio,et al.  Fermentative production of hydrogen from cassava processing wastewater by Clostridium acetobutylicum , 2011 .

[63]  D. Zilberman,et al.  Biofuel-related price transmission literature: A review , 2013 .

[64]  Jo-Shu Chang,et al.  Operation strategies for biohydrogen production with a high-rate anaerobic granular sludge bed bioreactor , 2004 .

[65]  K Bala Amutha,et al.  Biological hydrogen production by the algal biomass Chlorella vulgaris MSU 01 strain isolated from pond sediment. , 2011, Bioresource technology.

[66]  A. Melis,et al.  Green alga hydrogen production: progress, challenges and prospects , 2002 .

[67]  E. Greenbaum,et al.  A new oxygen sensitivity and its potential application in photosynthetic H2 production. , 2003, Applied biochemistry and biotechnology.

[68]  Michael Modigell,et al.  Comparison of two reactor concepts for anoxygenic H2 production by Rhodobacter capsulatus , 2010 .

[69]  Hamilton O. Smith,et al.  Heterologous Expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] Hydrogenases in Synechococcus elongatus , 2011, PloS one.

[70]  F. Kargı,et al.  Comparison of bio-hydrogen production from hydrolyzed wheat starch by mesophilic and thermophilic da , 2010 .

[71]  S. Bhattacharya,et al.  Hydrogen production by Cyanobacteria , 2005, Microbial Cell Factories.

[72]  Joo-Hwa Tay,et al.  Biohydrogen production: Current perspectives and the way forward , 2012 .

[73]  Yutaka Nakashimada,et al.  Enhanced hydrogen production in altered mixed acid fermentation of glucose by Enterobacter aerogenes , 1997 .

[74]  T. Antal,et al.  Production of H2 by sulphur‐deprived cells of the unicellular cyanobacteria Gloeocapsa alpicola and Synechocystis sp. PCC 6803 during dark incubation with methane or at various extracellular pH , 2005, Journal of applied microbiology.

[75]  Qiang Liao,et al.  Bioconversion characteristics of Rhodopseudomonas palustris CQK 01 entrapped in a photobioreactor for hydrogen production. , 2013, Bioresource technology.

[76]  Jo-Shu Chang,et al.  H2 production with anaerobic sludge using activated-carbon supported packed-bed bioreactors , 2004, Biotechnology Letters.

[77]  N. Ren,et al.  Optimization of Photo-Hydrogen Production by Immobilized Rhodopseudomonas Faecalis RLD-53 , 2011 .

[78]  Jo-Shu Chang,et al.  Biohydrogen production with fixed-bed bioreactors , 2002 .

[79]  P. Lindblad,et al.  HupW Protease Specifically Required for Processing of the Catalytic Subunit of the Uptake Hydrogenase in the Cyanobacterium Nostoc sp. Strain PCC 7120 , 2011, Applied and Environmental Microbiology.

[80]  D. O. Hall,et al.  Hydrogen metabolism of mutant forms of Anabaena variabilis in continuous cultures and under nutritional stress , 1997 .

[81]  T. Fukui,et al.  Continuous hydrogen production by the hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. , 2005, Journal of biotechnology.

[82]  Chiu-Yue Lin,et al.  Biohydrogen production using an up-flow anaerobic sludge blanket reactor , 2004 .

[83]  A. McDowall,et al.  Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion. , 2007, Plant biotechnology journal.

[84]  J. William Schopf,et al.  The Fossil Record: Tracing the Roots of the Cyanobacterial Lineage , 2000 .

[85]  Bal As,et al.  Upflow anaerobic sludge blanket reactor--a review. , 2001 .

[86]  J. Lay,et al.  Biohydrogen production as a function of pH and substrate concentration. , 2001, Environmental science & technology.

[87]  R. Ely,et al.  Photobiological hydrogen production from Synechocystis sp. PCC 6803 encapsulated in silica sol–gel , 2009 .

[88]  Paula Tamagnini,et al.  Cyanobacterial hydrogenases: diversity, regulation and applications. , 2007, FEMS microbiology reviews.

[89]  H. Sakurai,et al.  Disruption of the uptake hydrogenase gene, but not of the bidirectional hydrogenase gene, leads to enhanced photobiological hydrogen production by the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 , 2002, Applied Microbiology and Biotechnology.

[90]  Sheng-Shung Cheng,et al.  Effects of hydraulic retention time on anaerobic hydrogenation performance and microbial ecology of bioreactors fed with glucose–peptone and starch–peptone , 2010 .

[91]  Derek Tittle,et al.  The implications of using hydrocarbon fuels to generate electricity for hydrogen fuel powered automobiles on electrical capital, hydrocarbon consumption, and anthropogenic emissions , 2013 .

[92]  Ebru Özgür,et al.  Biohydrogen production by Rhodobacter capsulatus in solar tubular photobioreactor on thick juice dark fermenter effluent , 2012 .

[93]  J. Bockris,et al.  The origin of ideas on a Hydrogen Economy and its solution to the decay of the environment , 2002 .

[94]  O. Lenz,et al.  Oxygen Tolerance of the H2-sensing [NiFe] Hydrogenase from Ralstonia eutropha H16 Is Based on Limited Access of Oxygen to the Active Site* , 2005, Journal of Biological Chemistry.

[95]  E. Chan,et al.  Economics and environmental impact of bioethanol production technologies: an appraisal , 2007 .

[96]  Xing Yan,et al.  Effect of key factors on hydrogen production from cellulose in a co-culture of Clostridium thermocellum and Clostridium thermopalmarium. , 2010, Bioresource technology.

[97]  E. Sanhueza Agroetanol ¿un combustible ambientalmente amigable? , 2009 .

[98]  H. Argun,et al.  Bio-hydrogen production by different operational modes of dark and photo-fermentation: An overview , 2011 .

[99]  M. Ghirardi,et al.  Strategies for improving oxygen tolerance of algal hydrogen production , 2001 .

[100]  Y. Chisti,et al.  Comparative evaluation of compact photobioreactors for large-scale monoculture of microalgae , 1999 .

[101]  Photobiological hydrogen production and nitrogenase activity in some heterocystous cyanobacteria , 2001 .

[102]  Tristan R. Brown,et al.  Comparative techno-economic analysis of biohydrogen production via bio-oil gasification and bio-oil reforming. , 2013 .

[103]  E. Koukios,et al.  Integration of first and second generation biofuels: fermentative hydrogen production from wheat grain and straw. , 2013, Bioresource technology.

[104]  Shir-Ly Huang,et al.  Integrated Taguchi method and response surface methodology to confirm hydrogen production by anaerobic fermentation of cow manure , 2013 .

[105]  Patrick C. Hallenbeck,et al.  Biological hydrogen production; fundamentals and limiting processes , 2002 .

[106]  Hong Liu,et al.  Effect of pH on hydrogen production from glucose by a mixed culture. , 2002, Bioresource technology.

[107]  A. Kaminski,et al.  Hydrogenases in green algae: do they save the algae's life and solve our energy problems? , 2002, Trends in plant science.

[108]  Debabrata Das,et al.  Feasibility studies on the fermentative hydrogen production by recombinant Escherichia coli BL-21 , 2006 .

[109]  T. Masuda,et al.  Truncated chlorophyll antenna size of the photosystems—a practical method to improve microalgal productivity and hydrogen production in mass culture , 2002 .

[110]  H. Bui,et al.  Thermophilic fermentative hydrogen production from xylose by Thermotoga neapolitana DSM 4359 , 2012 .

[111]  Michael Seibert,et al.  Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells , 2006 .

[112]  Seeram Ramakrishna,et al.  Hydrogen photoproduction by use of photosynthetic organisms and biomimetic systems , 2009, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[113]  Michael Taylor,et al.  An overview of second generation biofuel technologies. , 2010, Bioresource technology.

[114]  Chiu-Yue Lin,et al.  Using sucrose as a substrate in an anaerobic hydrogen-producing reactor , 2003 .

[115]  M. Karp,et al.  Hydrogen production from glycerol using halophilic fermentative bacteria. , 2010, Bioresource technology.

[116]  Debabrata Das,et al.  Redirection of biochemical pathways for the enhancement of H2 production by Enterobacter cloacae , 2001, Biotechnology Letters.

[117]  Jun Cheng,et al.  Combination of dark- and photo-fermentation to improve hydrogen production from Arthrospira platensis wet biomass with ammonium removal by zeolite , 2012 .

[118]  Daniel C. Ducat,et al.  Rewiring hydrogenase-dependent redox circuits in cyanobacteria , 2011, Proceedings of the National Academy of Sciences.

[119]  Theodoros Damartzis,et al.  Thermochemical conversion of biomass to second generation biofuels through integrated process design—A review , 2011 .

[120]  Jean-Michel Lavoie,et al.  From first- to third-generation biofuels: Challenges of producing a commodity from a biomass of increasing complexity , 2013 .

[121]  M. K. Brennaman,et al.  Chemical approaches to artificial photosynthesis. 2. , 2005, Inorganic chemistry.

[122]  M. Nienoord,et al.  Energy aspects of biological hydrogen production in high rate bioreactors operated in the thermophilic temperature range , 2002 .

[123]  R. Wijffels,et al.  An Outlook on Microalgal Biofuels , 2010, Science.

[124]  Valentina Bosetti,et al.  Advanced Biofuels: Future Perspectives from an Expert Elicitation Survey , 2012 .

[125]  Bradley A. Saville,et al.  Introduction to chemical reaction engineering and kinetics , 1998 .

[126]  Gerasimos Lyberatos,et al.  Hydrogen and methane production through two-stage mesophilic anaerobic digestion of olive pulp. , 2009, Bioresource technology.

[127]  Yong Wang,et al.  Review of developments in portable hydrogen production using microreactor technology. , 2004, Chemical reviews.

[128]  Duu-Jong Lee,et al.  Bioreactor and process design for biohydrogen production. , 2011, Bioresource technology.

[129]  Sangeun Oh,et al.  The relative effectiveness of pH control and heat treatment for enhancing biohydrogen gas production. , 2003, Environmental science & technology.

[130]  Hang-Sik Shin,et al.  Continuous fermentative hydrogen production from coffee drink manufacturing wastewater by applying UASB reactor , 2010 .

[131]  A. Melis,et al.  Hydrogen production. Green algae as a source of energy. , 2001, Plant physiology.

[132]  F. Kargı,et al.  Bio-hydrogen production from waste materials , 2006 .

[133]  Jo-Shu Chang,et al.  Biohydrogen production using sequential two-stage dark and photo fermentation processes , 2008 .

[134]  Y. Swesi,et al.  Purification process for chemical storage of hydrogen for fuel cell vehicles applications , 2007 .

[135]  Juanita Mathews,et al.  Metabolic pathway engineering for enhanced biohydrogen production , 2009 .

[136]  Duu-Jong Lee,et al.  Hydrogen production by Rhodopseudomonas palustris CQK 01 in a continuous photobioreactor with ultrasonic treatment , 2012 .

[137]  Debabrata Das,et al.  The Prospect of Purple Non-Sulfur (PNS) Photosynthetic Bacteria for Hydrogen Production: The Present State of the Art , 2007 .

[138]  Yan Lin,et al.  Ethanol fermentation from biomass resources: current state and prospects , 2006, Applied Microbiology and Biotechnology.

[139]  Carla Silva,et al.  Biological hydrogen production by Anabaena sp. – Yield, energy and CO2 analysis including fermentative biomass recovery , 2012 .

[140]  Wei Zhang,et al.  Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis , 2004 .

[141]  Dipankar Ghosh,et al.  Advances in fermentative biohydrogen production: the way forward? , 2009, Trends in biotechnology.

[142]  Gergely Maróti,et al.  A novel approach for biohydrogen production , 2006 .

[143]  Lawrence Pitt,et al.  Biohydrogen production: prospects and limitations to practical application , 2004 .

[144]  Sharifah Rafidah Wan Alwi,et al.  A review on utilisation of biomass from rice industry as a source of renewable energy , 2012 .

[145]  Michel Pottier Hydrogen Production and Storage , 1982 .

[146]  Wei-fei Song,et al.  Biohydrogen production by immobilized Chlorella sp. using cycles of oxygenic photosynthesis and anaerobiosis. , 2011, Bioresource technology.

[147]  Mohd Ali Hassan,et al.  Biohydrogen production from biomass and industrial wastes by dark fermentation , 2009 .

[148]  P. Claassen,et al.  Dark hydrogen fermentations , 2003 .

[149]  Mi‐Sun Kim,et al.  Enhancing photo-fermentative hydrogen production by Rhodobacter sphaeroides KD131 and its PHB synthase deleted-mutant from acetate and butyrate , 2011 .

[150]  M. Ghirardi,et al.  Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin:NADP+-oxidoreductase (FNR) enzymes in vitro , 2011, Proceedings of the National Academy of Sciences.

[151]  Manuel J T Carrondo,et al.  Biochemical engineering. , 2004, Current opinion in biotechnology.

[152]  Jae-Hoon Hwang,et al.  Feasibility of hydrogen production from ripened fruits by a combined two-stage (dark/dark) fermentation system. , 2011, Bioresource technology.

[153]  S Venkata Mohan,et al.  Biohydrogen production from chemical wastewater treatment in biofilm configured reactor operated in periodic discontinuous batch mode by selectively enriched anaerobic mixed consortia. , 2007, Water research.

[154]  V. Hacker,et al.  Storage and separation of hydrogen with the metal steam process , 2010 .

[155]  B. Lal,et al.  Impact of regulated pH on proto scale hydrogen production from xylose by an alkaline tolerant novel bacterial strain, Enterobacter cloacae DT-1 , 2013 .

[156]  R. Burris,et al.  Occurrence and localization of two distinct hydrogenases in the heterocystous cyanobacterium Anabaena sp. strain 7120 , 1981, Journal of bacteriology.

[157]  M. Ishida,et al.  A hydrogen purification and storage system using metal hydride , 2012 .

[158]  Kusum Lata,et al.  State-of-the-art of anaerobic digestion technology for industrial wastewater treatment , 2000 .

[159]  M. Zdujić,et al.  Hydrogen storage material based on LaNi5 alloy produced by mechanical alloying , 2001 .

[160]  Z. Wronski Materials for rechargeable batteries and clean hydrogen energy sources , 2001 .

[161]  Tatsuki Wakayama,et al.  Efficient hydrogen production using a multi-layered photobioreactor and a photosynthetic bacterium mutant with reduced pigment , 2006 .

[162]  Boris I. Yakobson,et al.  Hydrogen, Fuel Cells and Infrastructure Technologies Program Multi-Year Research, Development and Demonstration Plan: , 2010 .

[163]  Jeongsik Kim,et al.  Effects of various pretreatments for enhanced anaerobic digestion with waste activated sludge. , 2003, Journal of bioscience and bioengineering.

[164]  Hanqing Yu,et al.  Thermophilic fermentative hydrogen production from starch-wastewater with bio-granules , 2009 .

[165]  D. H. Bradhurst,et al.  HYDROGEN PRODUCTION AND STORAGE , 1981 .

[166]  Olaf Kruse,et al.  Improved Photobiological H2 Production in Engineered Green Algal Cells* , 2005, Journal of Biological Chemistry.

[168]  A. Melis,et al.  Photobiological hydrogen production: Recent advances and state of the art. , 2011, Bioresource technology.

[169]  Amit Kumar,et al.  Large-scale biohydrogen production from bio-oil. , 2010, Bioresource technology.

[170]  Hyung-Sool Lee,et al.  Biological hydrogen production: prospects and challenges. , 2010, Trends in biotechnology.

[171]  Michael Seibert,et al.  Prolongation of H2 photoproduction by immobilized, sulfur-limited Chlamydomonas reinhardtii cultures. , 2008, Journal of biotechnology.

[172]  Y. Asada,et al.  Heterologous expression of clostridial hydrogenase in the Cyanobacterium synechococcus PCC7942. , 2000, Biochimica et biophysica acta.

[173]  Duu-Jong Lee,et al.  Dark fermentation on biohydrogen production: Pure culture. , 2011, Bioresource technology.