Hypoglycemic and Hepatoprotective Effects of Vernonia Amygdalina (Bitter Leaf) and Its Effect on Some Biochemical Parameters in Alloxan-induced Diabetic Male Albino Rats

The effects of methanolic leaf extract of Vernonia amygdalina (bitter leaf) on some biochemical parameters on alloxan-induced diabetic male albino rats (Wistar strain) were investigated for a period of 14 days. Adult male albino rats weighing between 140-160 g were induced intraperitoneally with alloxan. The male albino rats were grouped into five groups: Group A are not induced with alloxan, Group B serves as the negative control, Group C serves as positive control and was treated with glibenclaimide, Group D and E were treated with 100 and 200 mg/kg body weight of methanolic leaf extract of V. amygdalina respectively. The extracts were given to the animals orally for 14 days. The phytochemical analysis of Vernonia amygdalina extract indicates the presence of secondary metabolites like tannins, saponins, alkaloids, flavonoids, anthraquinones, Glycosides, Cyanogenic glycosides, polyphenols and Terpenoids. The weight of diabetic untreated rats (Group B) were significantly (P<0.005) reduced when compared to other groups. The group of rats given 100 and 200mg/Kg B.W of extract showed significant decrease (P<0.05) of blood sugar level compared to the untreated rats. The decrease in the blood glucose level of the rats following the administration of the plant extract suggests that the plant extract possesses anti-diabetic, anti-hyperglycemic and hypoglycemic effects on alloxan- induced diabetic rats. The extract of V. amygdalina produces hypolipidaemic effect and this is evident as there is a significant decrease in plasma TC, TG, LDC-Cholesterol, atherogenic index and an increase HDL-Cholesterol in the treated groups compared to the negative control group. The extracts significantly increase HGB, RBC, HCT and the WBC is significantly reduced in the treated group compared to the untreated group. This is an indication that the extract does possess hematopoietic activities and is not hematotoxic. The extract significantly reduced (P<0.005) liver biomarker enzymes (AST, ALT, ALP and GGT), an indication that it has hepatoprotective effect.

[1]  C. Ejike,et al.  Current perspectives on the medicinal potentials of Vernonia amygdalina Del. , 2011 .

[2]  R. Okigbo,et al.  Advances in selected medicinal and aromatic plants indigenous to Africa. , 2009 .

[3]  I. Atangwho,et al.  The Antidiabetic Efficacy of Combined Extracts from Two Continental Plants: Azadirachta indica (A. Juss) (Neem) and Vernonia amygdalina (Del.) (African Bitter Leaf) , 2008 .

[4]  D. Bressler,et al.  Nutritional composition of Gongronema latifolium and Vernonia amygdalina , 2008 .

[5]  M. C. Moses,et al.  Nutritional Components of Some Non-Conventional Leafy Vegetables Consumed in Cameroon , 2007 .

[6]  Paul Giboney,et al.  Mildly elevated liver transaminase levels in the asymptomatic patient. , 2005, American family physician.

[7]  L. Pari,et al.  Antihyperglycaemic effect of Cassia auriculata in experimental diabetes and its effects on key metabolic enzymes involved in carbohydrate metabolism , 2003, Clinical and experimental pharmacology & physiology.

[8]  S. Venkateswaran,et al.  Hypoglycaemic activity of Scopariadulcis L. extract in alloxan induced hyperglycaemic rats , 2002, Phytotherapy research : PTR.

[9]  A. Tiwari,et al.  Diabetes mellitus and multiple therapeutic approaches of phytochemicals: Present status and future prospects , 2002 .

[10]  L. Pari,et al.  Effect of Coccinia indica on Blood Glucose, Insulin and Key Hepatic Enzymes in Experimental Diabetes , 2002 .

[11]  M. Polenaković,et al.  Is erythropoietin a survival factor for red blood cells? , 1996, Journal of the American Society of Nephrology : JASN.

[12]  A. Tuah,et al.  Vernonia amygdalina as a supplement to teff straw (Eragrostis tef) fed to Ethiopian Menz sheep , 1995, Agroforestry Systems.

[13]  C. Bailey,et al.  Traditional plant treatments for diabetes. Studies in normal and streptozotocin diabetic mice , 1990, Diabetologia.

[14]  R. Levy,et al.  Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. , 1972, Clinical chemistry.

[15]  H. Nwanjo,et al.  Is Vernonia amygdalina hepatotoxic or hepatoprotective? Response from biochemical and toxicity studies in rats , 2006 .

[16]  Merlin C. Thomas,et al.  Anaemia in diabetes: an emerging complication of microvascular disease. , 2005, Current diabetes reviews.

[17]  S. Orkin,et al.  Hematopoiesis: how does it happen? , 1995, Current opinion in cell biology.

[18]  Abayomi Sofowora,et al.  Medicinal plants and traditional medicine in Africa , 1982 .

[19]  William Francis Ganong,et al.  Review of Medical Physiology , 1969 .