Integration of reverse transcriptase loop-mediated isothermal amplification with an immunochromatographic strip on a centrifugal microdevice for influenza A virus identification.

A novel centrifugal microdevice which could perform reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and immunochromatographic strip (ICS) based amplicon detection was demonstrated for simple and cost-effective influenza A virus identification. The proposed centrifugal microdevice consists of the sample and running buffer loading reservoirs, the RT-LAMP chamber, and the ICS for detecting gene expression. The entire process could be completed sequentially and automatically by simply controlling the rotation speed and by optimizing the microfluidic design. Monoplex and multiplex RT-LAMP reactions targeting H1 and/or M gene were executed at 66 °C for 40 min, and the resultant amplicons were successfully analysed on the ICS within 15 min. Influenza A H1N1 virus was subtyped by detecting H1 and M gene on the ICS even with 10 copies of viral RNAs. Highly specific and multiplex viral typing of the integrated RT-LAMP-ICS microdevice was also demonstrated. The combination of the rapid isothermal amplification with the simple colorimetric detection on a strip in a single centrifugal microdevice will provide an advanced genetic analysis platform in the field of on-site pathogen diagnostics.

[1]  Roland Zengerle,et al.  Microstructuring of polymer films for sensitive genotyping by real-time PCR on a centrifugal microfluidic platform. , 2010, Lab on a chip.

[2]  Marc Madou,et al.  Lab on a CD. , 2006, Annual review of biomedical engineering.

[3]  Yasuyoshi Mori,et al.  Real-time turbidimetry of LAMP reaction for quantifying template DNA. , 2004, Journal of biochemical and biophysical methods.

[4]  Bin Wu,et al.  Rapid and Sensitive Detection of Novel Avian-Origin Influenza A (H7N9) Virus by Reverse Transcription Loop-Mediated Isothermal Amplification Combined with a Lateral-Flow Device , 2013, PloS one.

[5]  Jie Dong,et al.  Human Infection with a Novel Avian-Origin Influenza A (H7N9) Virus. , 2018 .

[6]  S. Haswell,et al.  Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device. , 2009, Lab on a chip.

[7]  Peter J. Asiello,et al.  Miniaturized isothermal nucleic acid amplification, a review. , 2011, Lab on a chip.

[8]  Hui Chen,et al.  Predicting viruses accurately by a multiplex microfluidic loop-mediated isothermal amplification chip. , 2011, Analytical chemistry.

[9]  A. Nitsche,et al.  Diagnostic Approach for the Differentiation of the Pandemic Influenza A(H1N1)v Virus from Recent Human Influenza Viruses by Real-Time PCR , 2010, PloS one.

[10]  Doyoung Byun,et al.  A disposable, self-contained PCR chip. , 2009, Lab on a chip.

[11]  A. García-Sastre,et al.  Influenza A viruses: new research developments , 2011, Nature Reviews Microbiology.

[12]  A. Fauci,et al.  The next influenza pandemic: can it be predicted? , 2007, JAMA.

[13]  Yasuyoshi Mori,et al.  Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products , 2008, Nature Protocols.

[14]  Yong Tae Kim,et al.  A packaged paper fluidic-based microdevice for detecting gene expression of influenza A virus. , 2014, Biosensors & bioelectronics.

[15]  Tae Seok Seo,et al.  Combination of multiplex reverse-transcription loop-mediated isothermal amplification with an immunochromatographic strip for subtyping influenza A virus , 2014, Analytica Chimica Acta.

[16]  E. Starick,et al.  Type- and subtype-specific RT-PCR assays for avian influenza A viruses (AIV). , 2000, Journal of veterinary medicine. B, Infectious diseases and veterinary public health.

[17]  Gwo-Bin Lee,et al.  An integrated microfluidic loop-mediated-isothermal-amplification system for rapid sample pre-treatment and detection of viruses. , 2011, Biosensors & bioelectronics.

[18]  Ali Kemal Yetisen,et al.  Paper-based microfluidic point-of-care diagnostic devices. , 2013, Lab on a chip.

[19]  Yoon-Kyoung Cho,et al.  One-Step Pathogen Specific DNA Extraction from Whole Blood on a Centrifugal Microfluidic Device , 2007, TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference.

[20]  Tae Seok Seo,et al.  A rotary microsystem for simple, rapid and automatic RNA purification. , 2012, Lab on a chip.

[21]  Yi Sun,et al.  A lab-on-a-chip device for rapid identification of avian influenza viral RNA by solid-phase PCR. , 2011, Lab on a Chip.

[22]  Masahiro Ito,et al.  Rapid detection and typing of influenza A and B by loop-mediated isothermal amplification: comparison with immunochromatography and virus isolation. , 2006, Journal of virological methods.

[23]  T. Notomi,et al.  Loop-mediated isothermal amplification of DNA. , 2000, Nucleic acids research.

[24]  Mehmet Toner,et al.  Concentration and purification of human immunodeficiency virus type 1 virions by microfluidic separation of superparamagnetic nanoparticles. , 2010, Analytical chemistry.

[25]  Yajie Wang,et al.  Antigen detection based on background fluorescence quenching immunochromatographic assay. , 2014, Analytica chimica acta.

[26]  Gavin J. D. Smith,et al.  Reassortment of Pandemic H1N1/2009 Influenza A Virus in Swine , 2010, Science.

[27]  Young Ki Choi,et al.  Ultrafast rotary PCR system for multiple influenza viral RNA detection. , 2012, Lab on a chip.

[28]  Josiane P Lafleur,et al.  Miniaturised centrifugal solid phase extraction platforms for in-field sampling, pre-concentration and spectrometric detection of organic pollutants in aqueous samples. , 2010, Talanta.

[29]  Y. Mori,et al.  Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases , 2009, Journal of Infection and Chemotherapy.

[30]  Susana López,et al.  Molecular anatomy of 2009 influenza virus A (H1N1). , 2009, Archives of medical research.

[31]  Young Ki Choi,et al.  A microbead-incorporated centrifugal sample pretreatment microdevice. , 2013, Lab on a chip.

[32]  D. P. King,et al.  Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus , 2006, Archives of Virology.

[33]  Manmohan Parida,et al.  Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases , 2008, Reviews in medical virology.

[34]  Shijia Yang,et al.  Highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol. , 2014, Analytica chimica acta.

[35]  Y. Guan,et al.  Universal primer set for the full-length amplification of all influenza A viruses , 2001, Archives of Virology.

[36]  K. Gruden,et al.  Alternative DNA amplification methods to PCR and their application in GMO detection: a review , 2008 .

[37]  Tsugunori Notomi,et al.  Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus , 2004, Journal of Clinical Microbiology.

[38]  Jintae Kim,et al.  Centrifugal microfluidics for biomedical applications. , 2010, Lab on a chip.