Application of Propolis Extract in Gelatin Coatings as Environmentally Friendly Method for Extending the Shelf Life of Pork Loin

This study evaluates the effects of gelatin coating enriched with ethanolic propolis extract (PE) at 1%, 2% or 3% (w/v) on the quality parameters of pork meat during storage at 2 °C. Physical (pH, weight loss, color) and chemical parameters (percentage contents of metmyoglobin (MetMb), along with thiobarbituric acid reactive substances (TBARS)) were measured, and microbiological (total aerobic plate count (TAPC)) analysis, as well as consumer evaluation, was carried out every four days during the storage period of twelve days. The results indicated that the proposed treatments affected (p < 0.05) the quality characteristics of meat samples. The high prevention of physicochemical alterations and maximum inhibition of microorganisms was obtained for samples stored in gelatin coatings containing 2% and 3% PE. Additionally, despite a slight deterioration in odor on Day 4 in the P3 group, no negative changes in overall acceptability of the P2 and P3 groups compared to uncoated samples were observed. The obtained results indicate a significant role of propolis extract incorporation into gelatin packaging to extend the shelf life of stored pork.

[1]  Qiankun Wang,et al.  Fortification of edible films with bioactive agents: a review of their formation, properties, and application in food preservation , 2021, Critical reviews in food science and nutrition.

[2]  Jun Liu,et al.  Active packaging films and edible coatings based on polyphenol-rich propolis extract: A review. , 2021, Comprehensive reviews in food science and food safety.

[3]  P. Variyar,et al.  Pullulan or chitosan based active coating by incorporating polyphenols from lemon peel in raw poultry meat , 2020, Journal of Food Science and Technology.

[4]  M. Fabra,et al.  Antifungal edible coatings containing Argentinian propolis extract and their application in raspberries , 2020, Food Hydrocolloids.

[5]  A. Rafati,et al.  Shelf Life Extension of Veal Meat by Edible Coating Incorporated with Zataria multiflora Essential Oil , 2020 .

[6]  T. Hagiwara,et al.  Characterization of fish collagen from blue shark skin and its application for chitosan- collagen composite coating to preserve red porgy (Pagrus major) meat. , 2020, Journal of food biochemistry.

[7]  F. Toldrá,et al.  Effect of Gelatin Coating Enriched with Antioxidant Tomato By-Products on the Quality of Pork Meat , 2020, Polymers.

[8]  T. Krystofiak,et al.  Effect of accelerated aging on the color changes of wood treated with eco-friendly formulations based on propolis and silicon compounds , 2020, BioResources.

[9]  Huiyun Zhang,et al.  Effect of chitosan-gelatin coating containing nano-encapsulated tarragon essential oil on the preservation of pork slices. , 2020, Meat science.

[10]  Z. Correa-Pacheco,et al.  Effect of Nanostructured Chitosan/Propolis Coatings on the Quality and Antioxidant Capacity of Strawberries During Storage , 2020 .

[11]  A. Wierzbicka,et al.  The influence of sage and hemp oils addition to gelatin-based edible coating on the quality features of pork , 2020 .

[12]  Zaib Jahan,et al.  PVA/starch/propolis/anthocyanins rosemary extract composite films as active and intelligent food packaging materials , 2020, Journal of Food Safety.

[13]  T. Mehdizadeh,et al.  Chitosan coatings incorporated with propolis extract and Zataria multiflora Boiss oil for active packaging of chicken breast meat. , 2019, International journal of biological macromolecules.

[14]  A. Khodanazary,et al.  The shelf life extension of refrigerated Nemipterus japonicus fillets by chitosan coating incorporated with propolis extract. , 2019, International journal of biological macromolecules.

[15]  G. Brandão,et al.  Development of propolis nanoemulsion with antioxidant and antimicrobial activity for use as a potential natural preservative. , 2019, Food chemistry.

[16]  K. C. Mousinho,et al.  Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas. , 2019, Brazilian journal of biology = Revista brasleira de biologia.

[17]  Pavan Kumar,et al.  Antioxidant and Antimicrobial Activity of Porcine Liver Hydrolysate in Meat Emulsion and Their Influence on Physico-Chemical and Color Deterioration During Refrigeration Storage. , 2019, Journal of food science.

[18]  Armando Abel Massingue,et al.  Retail display of beef steaks coated with monolayer and bilayer chitosan-gelatin composites. , 2019, Meat science.

[19]  A. Khodanazary,et al.  Shelf Life of Saurida tumbil during Storage at Refrigeration Condition as affected by Gelatin-Based Edible Coatings Incorporated with propolis Extract , 2019, International Journal of Food Properties.

[20]  Khalid Ali Khan,et al.  Composition and functional properties of propolis (bee glue): A review , 2018, Saudi journal of biological sciences.

[21]  C. Fuenmayor,et al.  Effect of chitosan‐propolis edible coatings on stability of refrigerated cachama (Piaractus brachypomus) vacuum‐packed fish fillets , 2018, Packaging Technology and Science.

[22]  A. Wierzbicka,et al.  Evaluation of the antioxidant, anti-inflammatory and antimicrobial effects of catuaba, galangal, roseroot, maca root, guarana and polyfloral honey in sausages during storage , 2018, LWT.

[23]  M. J. Galotto,et al.  Development of poly(lactic acid) films with propolis as a source of active compounds: Biodegradability, physical, and functional properties , 2018, Journal of Applied Polymer Science.

[24]  R. Auras,et al.  Biodegradable Rice Starch/Carboxymethyl Chitosan Films with Added Propolis Extract for Potential Use as Active Food Packaging , 2018, Polymers.

[25]  B. Njari,et al.  The impact of frozen storage duration on physical, chemical and microbiological properties of pork. , 2018, Meat science.

[26]  C. Scarlett,et al.  Effect of Biocomposite Edible Coatings Based on Pea Starch and Guar Gum on Nutritional Quality of “Valencia” Orange During Storage , 2018 .

[27]  F. Toldrá,et al.  Effects of active gelatin coated with henna (L. inermis) extract on beef meat quality during chilled storage , 2018 .

[28]  K. Zia,et al.  Recent advances on polysaccharides, lipids and protein based edible films and coatings: A review. , 2017, International journal of biological macromolecules.

[29]  R. Ranjbar,et al.  The effect of chitosan coating incorporated with ethanolic extract of propolis on the quality of refrigerated chicken fillet , 2018 .

[30]  B. Devreese,et al.  Actin proteolysis during ripening of dry fermented sausages at different pH values. , 2017, Food chemistry.

[31]  S. Carpes,et al.  Physico-chemical characteristics of microencapsulated propolis co-product extract and its effect on storage stability of burger meat during storage at −15 °C , 2017 .

[32]  Y. Shahbazi,et al.  Effect of PLA films containing propolis ethanolic extract, cellulose nanoparticle and Ziziphora clinopodioides essential oil on chemical, microbial and sensory properties of minced beef. , 2017, Meat science.

[33]  U. Siripatrawan,et al.  Improving functional properties of chitosan films as active food packaging by incorporating with propolis , 2016 .

[34]  M. C. Garrigós,et al.  Gelatin-Based Films and Coatings for Food Packaging Applications , 2016 .

[35]  A. Rizzolo,et al.  Volatile compound composition and antioxidant activity of cooked ham slices packed in propolis-based active packaging , 2016 .

[36]  S. M. Osés,et al.  Bioactive properties of honey with propolis. , 2016, Food chemistry.

[37]  O. H. Gonçalves,et al.  Physical, antimicrobial and antioxidant properties of starch-based film containing ethanolic propolis extract , 2015 .

[38]  S. Popović,et al.  Edible films and coatings: Sources, properties and application , 2015 .

[39]  A. González-Córdova,et al.  Antioxidant and antimicrobial activity of commercial propolis extract in beef patties. , 2014, Journal of food science.

[40]  G. González-Aguilar,et al.  Edible coatings as encapsulating matrices for bioactive compounds: a review , 2014, Journal of Food Science and Technology.

[41]  Asgar Ali,et al.  Efficacy of Propolis and Cinnamon Oil Coating in Controlling Post-Harvest Anthracnose and Quality of Chilli (Capsicum annuum L.) during Cold Storage , 2014, Food and Bioprocess Technology.

[42]  P. Pajak,et al.  Opakowania jadalne na bazie białek i polisacharydów - charakterystyka i zastosowanie , 2013 .

[43]  T. Wittaya.  Protein-Based Edible Films: Characteristics and Improvement of Properties , 2012 .

[44]  A. Chiralt,et al.  Edible films and coatings to prevent the detrimental effect of oxygen on food quality: Possibilities and limitations , 2012 .

[45]  Md. Sazedul Hoque,et al.  Properties of blend film based on cuttlefish (Sepia pharaonis) skin gelatin and mungbean protein isolate. , 2011, International journal of biological macromolecules.

[46]  J. Gómez-Estaca,et al.  Effects of gelatin origin, bovine-hide and tuna-skin, on the properties of compound gelatin—chitosan films , 2011 .

[47]  A. Jiménez,et al.  Edible films and coatings: Structures, active functions and trends in their use , 2011 .

[48]  Sabina Galus,et al.  EFFECT OF PROTEIN CONCENTRATION ON KINETICS OF WATER VAPOUR ADSORPTION BY COATINGS PREPARED ON THE BASIS OF WHEY PROTEIN ISOLATE , 2011 .

[49]  J. Herring,et al.  Oxidative stability of gelatin coated pork at refrigerated storage. , 2010, Meat science.

[50]  S. Barringer,et al.  Meat Shelf-life and Extension using Collagen/Gelatin Coatings: A Review , 2010, Critical reviews in food science and nutrition.

[51]  M. Fik,et al.  Microbiological and Sensory Changes in Minced Beef Treated with Potassium Lactate and Sodium Diacetate during Refrigerated Storage , 2007 .

[52]  F. Villani,et al.  Changes in the Spoilage-Related Microbiota of Beef during Refrigerated Storage under Different Packaging Conditions , 2006, Applied and Environmental Microbiology.

[53]  Cameron Faustman,et al.  Krzywicki Revisited: Equations for Spectrophotometric Determination of Myoglobin Redox Forms in Aqueous Meat Extracts , 2006 .

[54]  P. Bechtel,et al.  Water vapor permeability of mammalian and fish gelatin films , 2006 .

[55]  H. Ashida,et al.  Antioxidative bioavailability of artepillin C in Brazilian propolis. , 2004, Archives of biochemistry and biophysics.

[56]  S. Kumazawa,et al.  Antioxidant activity of propolis of various geographic origins , 2004 .

[57]  Shigetoshi Kadota,et al.  Recent progress in pharmacological research of propolis , 2001, Phytotherapy research : PTR.

[58]  Jan Pikul,et al.  Evaluation of three modified TBA methods for measuring lipid oxidation in chicken meat , 1989 .

[59]  K. Krzywicki,et al.  Assessment of relative content of myoglobin, oxymyoglobin and metmyoglobin at the surface of beef. , 1979, Meat science.