Diurnal variations in methionine content and expression of certain genes involved in DNA methylation reaction in pigs

Abstract Whether methionine content and its metabolites in liver and skeletal muscle show diurnal rhythmicity remains unknown. In the present study, we investigated the diurnal rhythms in methionine and S-adenosylmethionine (SAM) in plasma, liver, and longissimus thoracis muscle, as well as in enzymes involved in methionine-related transmethylation reaction using pigs maintained in a 12-h photoperiod. The results showed that concentration of methionine and SAM, as well as mRNA expression of methionine adenosyltransferase (MAT1A), DNA methyltranferase (DNMT3A and DNMT3B) in liver showed diurnal rhythmicity. Comparison with typical circadian clock genes revealed a similarity between the diurnal patterns of MAT1A and cryptochrome circadian clock 1 (CRY1) expression, whereas the daily patterns of MAT2A, DNMT3A, and DNMT3B expression were reversely related to the expression patterns of Timeless. These results imply that much attention should be paid to rhythms of methionine metabolism and methylation activity in the pathogenesis of diseases, such as cancer and metabolic syndrome.

[1]  Guoyao Wu,et al.  Methionine restriction on lipid metabolism and its possible mechanisms , 2016, Amino Acids.

[2]  Liuqin He,et al.  Effects of Alpha-Ketoglutarate on Glutamine Metabolism in Piglet Enterocytes in Vivo and in Vitro. , 2016, Journal of agricultural and food chemistry.

[3]  R. Bertolo,et al.  The dynamics of methionine supply and demand during early development. , 2016, Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme.

[4]  L. Heilbronn,et al.  Serum S-adenosylmethionine, but not methionine, increases in response to overfeeding in humans , 2016, Nutrition & Diabetes.

[5]  S. Shimba,et al.  Diurnal expression of Dnmt3b mRNA in mouse liver is regulated by feeding and hepatic clockwork , 2012, Epigenetics.

[6]  E. Blomstrand,et al.  Alterations in amino acid concentrations in the plasma and muscle in human subjects during 24 h of simulated adventure racing , 2012, European Journal of Applied Physiology.

[7]  N. Nishimura,et al.  Global DNA methylation in the mouse liver is affected by methyl deficiency and arsenic in a sex-dependent manner , 2011, Archives of Toxicology.

[8]  G. Wu,et al.  Developmental changes in the concentrations of glutamine and other amino acids in plasma and skeletal muscle of the Standardbred foal. , 2009, Journal of animal science.

[9]  Shelly C. Lu,et al.  Expansion of liver cancer stem cells during aging in methionine adenosyltransferase 1A–deficient mice , 2007, Hepatology.

[10]  Shelly C. Lu,et al.  Role of S‐adenosyl‐L‐methionine in liver health and injury , 2007, Hepatology.

[11]  M. A. Pereira,et al.  Reversed-phase high-performance liquid chromatography procedure for the simultaneous determination of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine in mouse liver and the effect of methionine on their concentrations. , 2001, Journal of chromatography. B, Biomedical sciences and applications.

[12]  Shelly C. Lu,et al.  Methionine adenosyltransferase 1A knockout mice are predisposed to liver injury and exhibit increased expression of genes involved in proliferation , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[13]  F. Corrales,et al.  Hysteretic Behavior of Methionine Adenosyltransferase III , 2000, The Journal of Biological Chemistry.

[14]  M. Kotb,et al.  Expression and Functional Interaction of the Catalytic and Regulatory Subunits of Human Methionine Adenosyltransferase in Mammalian Cells* , 1999, The Journal of Biological Chemistry.

[15]  C S Davidson,et al.  Diurnal variations in plasma concentrations of tryptophan, tryosine, and other neutral amino acids: effect of dietary protein intake. , 1979, The American journal of clinical nutrition.

[16]  W. Beisel,et al.  Circadian Periodicity of Blood Amino-acids in Normal and Adrenalectomized Mice , 1969, Nature.

[17]  R. Wurtman,et al.  Daily rhythms in the concentrations of various amino acids in human plasma. , 1968, The New England journal of medicine.

[18]  P. M. Voorhoeve,et al.  MicroRNAs: Oncogenes, tumor suppressors or master regulators of cancer heterogeneity? , 2010, Biochimica et biophysica acta.