Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov.

We studied the taxonomic positions of the rapidly growing organism Mycobacterium fortuitum and phenotypically related organisms. We confirmed that "Mycobacterium peregrinum" ATCC 14467T (T = type strain) is genetically independent of M. fortuitum ATCC 6841T by using various DNA hybridization conditions. Strains that were genetically identified as "M. peregrinum" were phenotypically differentiated from M. fortuitum ATCC 6841T. Thus, we propose that "M. peregrinum" should be revived as an independent species, Mycobacterium peregrinum sp. nov., nom. rev. The type strain is strain ATCC 14467. M. fortuitum subsp. acetamidolyticum ATCC 35931T exhibited a high level of DNA relatedness to M. fortuitum ATCC 6841T. The hybridized DNAs maintained stable heteroduplexity at high stringency; thus, we confirmed that M. fortuitum subsp. acetamidolyticum is identical to M. fortuitum ATCC 6841T. We found that M. chelonae subsp. abscessus ATCC 19977T is genetically different from M. chelonae subsp. chelonae NCTC 946T on the basis of the results of quantitative hybridization even under optimal conditions. There was no reason to maintain this organism as a subspecies of M. chelonae. Thus, we propose that M. chelonae subsp. abscessus should be elevated to species status as Mycobacterium abscessus (Kubica et al.) comb. nov. The type strain is strain ATCC 19977.

[1]  J. Stanford,et al.  Serological and Bacteriological Investigation of Mycobacterium ranae (fortuitum) , 1969, Journal of bacteriology.

[2]  Takayuki Ezaki,et al.  Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains , 1989 .

[3]  M. Tsukamura,et al.  Identification of mycobacteria. , 1967, Tubercle.

[4]  P. Sneath,et al.  Approved lists of bacterial names. , 1980, The Medical journal of Australia.

[5]  F. Grimont,et al.  Deoxyribonucleic Acid Relatedness Study of the Mycobacterium fortuitum-Mycobacterium chelonae Complex , 1986 .

[6]  M. Tsukamura.  Adansonian classification of mycobacteria. , 1966, Journal of general microbiology.

[7]  T. Ezaki,et al.  Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species , 1991, Journal of clinical microbiology.

[8]  S. Imaeda,et al.  Genomic Relatedness among Mycobacterial Species by Nonisotopic Blot Hybridization , 1988 .

[9]  I. Yano,et al.  Mycobacterium fortuiturn Subspecies acetamidolyticum, a New Subspecies of Mycobacterium fortuiturn , 1986, Microbiology and immunology.

[10]  P. Brennan,et al.  Antigenic Relationships of the Mycobacterium fortuitum-Mycobacterium chelonae Complex , 1984 .

[11]  J. Grange,et al.  A study of Mycobacterium fortuitum (ranae). , 1974, Journal of medical microbiology.

[12]  L. G. Wayne,et al.  Diagnostic key to mycobacteria encountered in clinical laboratories. , 1968, Applied microbiology.

[13]  I. Baess.  Deoxyribonucleic acid relatedness among species of rapidly growing mycobacteria. , 2009, Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology.

[14]  M. Tsukamura.  Differentiation of Mycobacteria by Susceptibility to Hydroxylamine and 8-Azaguanine , 1965, Journal of bacteriology.

[15]  P. Doty,et al.  Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. , 1962, Journal of molecular biology.

[16]  O. Kandler,et al.  International Committee on Systematic Bacteriology: announcement of the report of the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. , 1987, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology.