An Electrochemical Biosensor with Cholesterol Oxidase/ Sol‐Gel Film on a Nanoplatinum/Carbon Nanotube Electrode

The carbon nanotubes decorated nanoplatinum (CNT-Pt) were prepared using a chemical reduction method and a novel base electrode was constructed by intercalating CNT-Pt on the surface of a waxed graphite electrode. The results showed that the nano-particles of platinum at a waxed graphite electrode exhibits high catalytic activity for the reduction of hydrogen peroxide. The cholesterol oxidase (ChOx), chosen as a model enzyme, was immobilized with sol-gel on the CNT-Pt base electrode to construct a biosensor. The current response of the biosensor for cholesterol was very rapid (<20 s). The linear range for cholesterol measurement was 4.0×10−6 mol/L −1.0×10−4 mol/L with a detection limit of 1.4×10−6 mol/L. The experiments also showed that the ChOx/sol-gel/CNT-Pt biosensor was sensitive and stable in detecting cholesterol in serum samples.

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