Placental‐like alkaline phosphatase and DNA flow cytometry in spermatocytic seminoma

Immunohistochemical analysis was done on 7 testicular tumors classified as spermatocytic seminoma (SS) and 25 classic seminomas. Except for a few scattered cells, the spermatocytic seminomas were negative for placental‐like alkaline phosphatase (PLAP); the classic seminomas were all positive for this enzyme. The SS also were negative for alpha‐fetoprotein (AFP), beta‐human chorionic gonadotropin (hCG), and leukocyte common antigen (LCA). The ploidy of the seven tumors of SS was as follows: two, diploid; two, near‐diploid; one, tetraploid; one, aneuploid; and one, uninterpretable. The essentially negative staining of SS for PLAP was strikingly different from the pattern in classic seminoma. Thus, staining for this enzyme is useful for making the differential diagnosis between classic seminoma and SS. To differentiate between malignant lymphoma and SS, staining for leukocyte common antigen is helpful. Cancer 1992; 69:993–996.

[1]  D. Sleijfer,et al.  Ploidy of primary germ cell tumors of the testis. Pathogenetic and clinical relevance. , 1989, Laboratory investigation; a journal of technical methods and pathology.

[2]  I. Damjanov.  Is seminoma a relative or a precursor of embryonal carcinoma? , 1989, Laboratory investigation; a journal of technical methods and pathology.

[3]  D. Ondruš,et al.  Metastatic spermatocytic seminoma. A case report with light microscopic, ultrastructural, and immunohistochemical findings , 1988, Cancer.

[4]  F. Mostofi,et al.  Placental alkaline phosphatase immunohistochemistry of intratubular malignant germ cells and associated testicular germ cell tumors. , 1988, Human pathology.

[5]  H. Joensuu,et al.  Comparison of nuclear DNA content in primary and metastatic differentiated thyroid carcinoma. , 1988, American journal of clinical pathology.

[6]  O. Koide,et al.  Identification of testicular atypical germ cells by an immunohistochemical technique for placental alkaline phosphatase , 1987, Cancer.

[7]  N. Skakkebaek,et al.  Carcinoma-in-situ of the testis: possible origin from gonocytes and precursor of all types of germ cell tumours except spermatocytoma. , 1987, International journal of andrology.

[8]  N. Skakkebaek,et al.  The spermatocytic seminoma: views on pathogenesis. , 1987, International journal of andrology.

[9]  M. Wick,et al.  Placental Alkaline Phosphatase Immunoreactivity in Testicular Germ-Cell Neoplasms , 1987, The American journal of surgical pathology.

[10]  B. No̸rgaard-Pedersen,et al.  Placental alkaline phosphatase in testicular germ cell tumours and in carcinoma-in-situ of the testis. An immunohistochemical study. , 2009, Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, Pathology.

[11]  A. Talerman,et al.  Spermatocytic seminoma: ultrastructural and microspectrophotometric observations , 1984 .

[12]  H Stein,et al.  Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). , 1984, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[13]  I W Taylor,et al.  Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[14]  G. Jacobsen,et al.  Carcinoma in situ of testicular tissue adjacent to malignant germ‐cell tumors: A study of 105 cases , 1981, Cancer.

[15]  A. Talerman.  Spermatocytic seminoma. Clinicopathological study of 22 cases , 1980, Cancer.

[16]  J. Rosai,et al.  Spermatocytic seminoma. II. Ultrastructural study , 1969, Cancer.