Thermal transitions in mixtures of polydeoxyribodinucleotides

Oligo d(C‐A) and oligo d(T‐G) of known average lengths, prepared by a combination of chemical and enzymatic procedures, have been mixed in 0.02 M and 0.07 M Na+, and absorbance has been studied as a function of increasing temperature. The transitions have been analyzed for the temperature of maximum slope Tm, the breadth of the transition, the value of the slope at Tm, and the maximum hyperchromicity. Linear expressions have been found relating the inverse of the length in nucleotide units (n−1) of the shorter oligomer, irrespective of its identity, to Tm and also to the transition breadth. From a difference in slope between the Tm versus n−1 expressions for the two molarities, the entropy and enthalpy of melting have been calculated as a function of n−1.

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