fMRI study of acupuncture-induced periaqueductal gray activity in humans

BOLD fMRI was used to study acupuncture-induced activation (increase in the BOLD signal from undetectable) of the periaqueductal gray (PAG) and two somatosensory cortical areas in seven healthy human subjects. Mechanical stimulation (push-pull) was given to the LI4 (Hoku) acupoint or to a non-acupoint. The stimulation paradigm consisted of 5 runs, each consisting of four 30 s On/30 s OFF periods over 30 min. The scan for each ON period was analyzed individually. The PAG and cortical areas showed different activity patterns. PAG activity was episodic and reliably demonstrated after 20–25 min of stimulation; both cortical areas, however, were active >90% of the time. Stimulation of a non-acupoint (leg) resulted in reduced levels of PAG and cortical activity.

[1]  Hong-wei Dong,et al.  Cholecystokinin antisense RNA increases the analgesic effect induced by electroacupuncture or low dose morphine: conversion of low responder rats into high responders , 1997, Pain.

[2]  Ravi S. Menon,et al.  Imaging Attentional Modulation of Pain in the Periaqueductal Gray in Humans , 2002, The Journal of Neuroscience.

[3]  B Carder,et al.  Analgesia from Electrical Stimulation in the Brainstem of the Rat , 1971, Science.

[4]  S. Anderson,et al.  On Acupuncture Analgeisa and the Mechanism of Pain , 1975 .

[5]  Jisheng Han.  Physiology of Acupuncture: Review of Thirty Years of Research , 1997 .

[6]  B. Rosen,et al.  Acupuncture modulates the limbic system and subcortical gray structures of the human brain: Evidence from fMRI studies in normal subjects , 2000, Human brain mapping.

[7]  M. Behbehani,et al.  An electrophysiological characterization of the projection from the central nucleus of the amygdala to the periaqueductal gray of the rat: the role of opioid receptors , 1995, Brain Research.

[8]  Satoru Miyauchi,et al.  Circulatory basis of fMRI signals: relationship between changes in the hemodynamic parameters and BOLD signal intensity , 2004, NeuroImage.

[9]  David Metcalf,et al.  A Digital Brain Atlas for Surgical Planning, Model-Driven Segmentation, and Teaching , 1996, IEEE Trans. Vis. Comput. Graph..

[10]  N. Canteras,et al.  Analgesia and c-Fos expression in the periaqueductal gray induced by electroacupuncture at the Zusanli point in rats , 2003, Brain Research.

[11]  P. Bellgowan,et al.  Antinociception following opioid stimulation of the basolateral amygdala is expressed through the periaqueductal gray and rostral ventromedial medulla , 1998, Brain Research.

[12]  D. Pfaff,et al.  Frequent colocalization of mu opioid and NMDA‐type glutamate receptors at postsynaptic sites in periaqueductal gray neurons , 1999, The Journal of comparative neurology.

[13]  R. Peyron,et al.  Functional imaging of brain responses to pain. A review and meta-analysis (2000) , 2000, Neurophysiologie Clinique/Clinical Neurophysiology.

[14]  C. Benedetti,et al.  Evoked potential assessment of acupunctural analgesia: Attempted reversal with naloxone , 1980, Pain.

[15]  Donald D. Price,et al.  Antagonism of acupuncture analgesia in man by the narcotic antagonist naloxone , 1977, Brain Research.

[16]  Karl J. Friston,et al.  Statistical parametric maps in functional imaging: A general linear approach , 1994 .

[17]  G. Mckhann,et al.  Central nervous system mechanisms of pain , 2002 .

[18]  K. Uğurbil,et al.  Experimental determination of the BOLD field strength dependence in vessels and tissue , 1997, Magnetic resonance in medicine.

[19]  W. Willis.  Central nervous system mechanisms for pain modulation. , 1985, Applied neurophysiology.

[20]  M. Torrens.  Co-Planar Stereotaxic Atlas of the Human Brain—3-Dimensional Proportional System: An Approach to Cerebral Imaging, J. Talairach, P. Tournoux. Georg Thieme Verlag, New York (1988), 122 pp., 130 figs. DM 268 , 1990 .