Droplet-based compartmentalization of chemically separated components in two-dimensional separations.

We demonstrate that nanolitre-sized droplets are an effective tool in coupling two-dimensional separations in both time and space. Using a microfluidic droplet connector, chemically separated components can be segmented into nanolitre droplets. After oil filtering and droplet merging, these droplets are loaded into a second dimension for comprehensive separations.

[1]  Igor L Medintz,et al.  Microfabricated two-dimensional electrophoresis device for differential protein expression profiling. , 2007, Analytical chemistry.

[2]  N Gottschlich,et al.  Two-dimensional electrochromatography/capillary electrophoresis on a microchip. , 2001, Analytical chemistry.

[3]  G. Smejkal,et al.  Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis. , 2006, Journal of proteome research.

[4]  Monpichar Srisa-Art,et al.  Microdroplets: a sea of applications? , 2008, Lab on a chip.

[5]  R. Bischoff,et al.  An automated on-line multidimensional HPLC system for protein and peptide mapping with integrated sample preparation. , 2002, Analytical chemistry.

[6]  Emily H Turner,et al.  Bioanalytical applications of capillary electrophoresis. , 2006, Analytical chemistry.

[7]  A. deMello,et al.  Pillar-induced droplet merging in microfluidic circuits. , 2008, Lab on a chip.

[8]  J. Yates,et al.  Large-scale analysis of the yeast proteome by multidimensional protein identification technology , 2001, Nature Biotechnology.

[9]  Yiqiong Zhao,et al.  Compartmentalization of chemically separated components into droplets. , 2009, Angewandte Chemie.

[10]  Yan Li,et al.  Integration of isoelectric focusing with parallel sodium dodecyl sulfate gel electrophoresis for multidimensional protein separations in a plastic microfludic network , 2004 .

[11]  H. Stone,et al.  Formation of dispersions using “flow focusing” in microchannels , 2003 .

[12]  Chaitanya P. Pabbati,et al.  Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis. , 2006, Analytical chemistry.

[13]  D. Weitz,et al.  Electric control of droplets in microfluidic devices. , 2006, Angewandte Chemie.

[14]  H. Cortes.  Multidimensional Chromatography , 2020 .

[15]  G. Whitesides,et al.  A prototype two-dimensional capillary electrophoresis system fabricated in poly(dimethylsiloxane). , 2002, Analytical chemistry.

[16]  J Michael Ramsey,et al.  High-efficiency, two-dimensional separations of protein digests on microfluidic devices. , 2003, Analytical chemistry.

[17]  B. Zhang,et al.  Single-particle fritting technology for capillary electrochromatography. , 2007, Analytical chemistry.

[18]  Emily H Turner,et al.  Chemical cytometry: fluorescence-based single-cell analysis. , 2008, Annual review of analytical chemistry.

[19]  G. Whitesides,et al.  Formation of droplets and bubbles in a microfluidic T-junction-scaling and mechanism of break-up. , 2006, Lab on a chip.

[20]  J. Giddings.  Concepts and comparisons in multidimensional separation , 1987 .

[21]  J. Jorgenson,et al.  Multidimensional LC-LC and LC-CE for high-resolution separations of biological molecules , 2004, Analytical and bioanalytical chemistry.

[22]  Robert T Kennedy,et al.  Sampling and electrophoretic analysis of segmented flow streams using virtual walls in a microfluidic device. , 2008, Analytical chemistry.

[23]  Xize Niu,et al.  Electro-coalescence of digitally controlled droplets. , 2009, Analytical chemistry.