Protective effect of melatonin on lung injury caused by ischemia-reperfusion of the lower extremities

Methods: Forty male New Zealand white rabbits were used in the study. Rabbits were randomly divided into four groups: Group 1 (sham group, n=10), group 2 (I-R group without treatment, n=10), group 3 (preischemia treatment group, n=10), group 4 (prereperfusion treatment group, n=10). Only anesthesia was performed to rabbits in group 1 and after 6 hours blood and tissue samples were taken. In groups 2, 3 and 4, after anesthesia tourniquets were applied to bilateral hind limbs at the hip level. After 4 hours of ischemia, the tourniquets were removed and following two hours of reperfusion period, blood and tissue samples were taken. Melatonin (10 mg/kg) was administered intraperitoneally 15 minutes before ischemia to group 3, and 15 minutes before reperfusion to group 4. Plasma malondialdehyde (MDA) levels were measured in blood samples and lung tissue MDA levels were determined. Additionally, lung tissue was examined histopathologically.

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