Tissue Reconstruction Based on Deformation of Dual Simplex Meshes

A new semiautomatic method for tissue reconstruction based on deformation of a dual simplex mesh was developed. The method is suitable for specifically-shaped objects. The method consists of three steps: the first step includes searching for object markers, i. e. the approximate centre of each object is localized. The searching procedure is based on careful analysis of object boundaries and on the assumption that the analyzed objects are sphere-like shaped. The first contribution of the method is the ability to find the markers without choosing the particular objects by hand.

[1]  Hans-Peter Kriegel,et al.  3D Shape Histograms for Similarity Search and Classification in Spatial Databases , 1999, SSD.

[2]  Frederic Devernay.  A Non-Maxima Suppression Method for Edge Detection with Sub-Pixel Accuracy , 1995 .

[3]  M Kozubek,et al.  High-resolution cytometry of FISH dots in interphase cell nuclei. , 1999, Cytometry.

[4]  Pavel Matula,et al.  Spherical Object Reconstruction Using Star-Shaped Simplex Meshes , 2001, EMMCVPR.

[5]  James A. Sethian,et al.  Level Set Methods and Fast Marching Methods , 1999 .

[6]  Demetri Terzopoulos,et al.  Signal matching through scale space , 1986, International Journal of Computer Vision.

[7]  Brian C. Lovell,et al.  Unsupervised cell nucleus segmentation with active contours , 1998, Signal Process..

[8]  K. Rodenacker,et al.  Groping for Quantitative Digital 3-D Image Analysis: An Approach to Quantitative Fluorescence In Situ Hybridization in Thick Tissue Sections of Prostate Carcinoma , 1997, Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology.

[9]  Pierre Soille,et al.  Morphological Image Analysis: Principles and Applications , 2003 .

[10]  Hervé Delingette,et al.  General Object Reconstruction Based on Simplex Meshes , 1999, International Journal of Computer Vision.

[11]  Steve R. Gunn,et al.  A Model Based Dual Active Contour , 1994, BMVC.

[12]  H. Netten,et al.  FISH and chips: automation of fluorescent dot counting in interphase cell nuclei. , 1997, Cytometry.

[13]  Jim R. Parker,et al.  Algorithms for image processing and computer vision , 1996 .

[14]  R. Malladi,et al.  Segmentation of nuclei and cells using membrane related protein markers , 2001, Journal of microscopy.