Extended and direct evaluation of RT‐QuIC assays for Creutzfeldt‐Jakob disease diagnosis

Real‐Time Quaking‐Induced Conversion (RT‐QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non‐prion disease patients, we provide the first direct and concurrent comparison of our improved RT‐QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2‐day reduction in average detection time, and 100% specificity.

[1]  L. Sacchetto,et al.  Diagnosis of Human Prion Disease Using Real-Time Quaking-Induced Conversion Testing of Olfactory Mucosa and Cerebrospinal Fluid Samples , 2017, JAMA neurology.

[2]  E. Hoover,et al.  Enhanced prion detection in biological samples by magnetic particle extraction and real-time quaking-induced conversion. , 2016, The Journal of general virology.

[3]  B. Caughey,et al.  Advanced tests for early and accurate diagnosis of Creutzfeldt–Jakob disease , 2016, Nature Reviews Neurology.

[4]  P. Cras,et al.  Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study , 2016, Annals of neurology.

[5]  B. Caughey,et al.  Factors That Improve RT-QuIC Detection of Prion Seeding Activity , 2016, Viruses.

[6]  W. D. Walter,et al.  Seeded Amplification of Chronic Wasting Disease Prions in Nasal Brushings and Recto-anal Mucosa-Associated Lymphoid Tissues from Elk by Real-Time Quaking-Induced Conversion , 2016, Journal of Clinical Microbiology.

[7]  B. Caughey,et al.  Detection of Atypical H-Type Bovine Spongiform Encephalopathy and Discrimination of Bovine Prion Strains by Real-Time Quaking-Induced Conversion , 2016, Journal of Clinical Microbiology.

[8]  B. Ghetti,et al.  Bank Vole Prion Protein As an Apparently Universal Substrate for RT-QuIC-Based Detection and Discrimination of Prion Strains , 2015, PLoS pathogens.

[9]  A. Karch,et al.  Stability and Reproducibility Underscore Utility of RT-QuIC for Diagnosis of Creutzfeldt-Jakob Disease , 2015, Molecular Neurobiology.

[10]  B. Caughey,et al.  Detection and Discrimination of Classical and Atypical L-Type Bovine Spongiform Encephalopathy by Real-Time Quaking-Induced Conversion , 2015, Journal of Clinical Microbiology.

[11]  B. Caughey,et al.  Rapid and Sensitive RT-QuIC Detection of Human Creutzfeldt-Jakob Disease Using Cerebrospinal Fluid , 2015, mBio.

[12]  V. Lal,et al.  A test for Creutzfeldt-Jakob disease using nasal brushings. , 2014, The New England journal of medicine.

[13]  A. Karch,et al.  Characteristic CSF Prion Seeding Efficiency in Humans with Prion Diseases , 2014, Molecular Neurobiology.

[14]  B. Caughey,et al.  Rapid Antemortem Detection of CWD Prions in Deer Saliva , 2013, PloS one.

[15]  I. Zerr,et al.  Early Detection of Abnormal Prion Protein in Genetic Human Prion Diseases Now Possible Using Real-Time QUIC Assay , 2013, PloS one.

[16]  Bob Will,et al.  RT-QuIC analysis of cerebrospinal fluid in sporadic Creutzfeldt-Jakob disease: a potential new diagnostic test , 2012 .

[17]  Mary Andrews,et al.  Real time quaking‐induced conversion analysis of cerebrospinal fluid in sporadic Creutzfeldt–Jakob disease , 2012, Annals of neurology.

[18]  L. Schonberger,et al.  Iatrogenic Creutzfeldt-Jakob Disease, Final Assessment , 2012, Emerging infectious diseases.

[19]  Steven J Collins,et al.  Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion , 2011, Nature Medicine.

[20]  Brent Race,et al.  Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays , 2010, PLoS pathogens.

[21]  C. Begue,et al.  Updated clinical diagnostic criteria for sporadic Creutzfeldt-Jakob disease , 2009, Brain : a journal of neurology.

[22]  David W. Colby,et al.  Prion detection by an amyloid seeding assay , 2007, Proceedings of the National Academy of Sciences.