An evidence-based review on the likely economic and environmental impact of genetically modified cereals and oilseeds for UK agriculture

An evidence-based review of the potential impact that the introduction of genetically-modified (GM) cereal and oilseed crops could have for the UK was carried out. The inter-disciplinary research project addressed the key research questions using scenarios for the uptake, or not, of GM technologies. This was followed by an extensive literature review, stakeholder consultation and financial modelling. The world area of canola, oilseed rape (OSR) low in both erucic acid in the oil and glucosinolates in the meal, was 34M ha in 2012 of which 27% was GM; Canada is the lead producer but it is also grown in the USA, Australia and Chile. Farm level effects of adopting GM OSR include: lower production costs; higher yields and profits; and ease of farm management. Growing GM OSR instead of conventional OSR reduces both herbicide usage and environmental impact. Some 170M ha of maize was grown in the world in 2011 of which 28% was GM; the main producers are the USA, China and Brazil. Spain is the main EU producer of GM maize although it is also grown widely in Portugal. Insect resistant (IR) and herbicide tolerant (HT) are the GM maize traits currently available commercially. Farm level benefits of adopting GM maize are lower costs of production through reduced use of pesticides and higher profits. GM maize adoption results in less pesticide usage than on conventional counterpart crops leading to less residues in food and animal feed and allowing increasing diversity of bees and other pollinators. In the EU, well-tried coexistence measures for growing GM crops in the proximity of conventional crops have avoided gene flow issues. Scientific evidence so far seems to indicate that there has been no environmental damage from growing GM crops. They may possibly even be beneficial to the environment as they result in less pesticides and herbicides being applied and improved carbon sequestration from less tillage. A review of work on GM cereals relevant for the UK found input trait work on: herbicide and pathogen tolerance; abiotic stress such as from drought or salinity; and yield traits under different field conditions. For output traits, work has mainly focussed on modifying the nutritional components of cereals and in connection with various enzymes, diagnostics and vaccines. Scrutiny of applications submitted for field trial testing of GM cereals found around 9000 applications in the USA, 15 in Australia and 10 in the EU since 1996. There have also been many patent applications and granted patents for GM cereals in the USA for both input and output traits;an indication of the scale of such work is the fact that in a 6 week period in the spring of 2013, 12 patents were granted relating to GM cereals. A dynamic financial model has enabled us to better understand and examine the likely performance of Bt maize and HT OSR for the south of the UK, if cultivation is permitted in the future. It was found that for continuous growing of Bt maize and HT OSR, unless there was pest pressure for the former and weed pressure for the latter, the seed premia and likely coexistence costs for a buffer zone between other crops would reduce the financial returns for the GM crops compared with their conventional counterparts. When modelling HT OSR in a four crop rotation, it was found that gross margins increased significantly at the higher levels of such pest or weed pressure, particularly for farm businesses with larger fields where coexistence costs would be scaled down. The impact of the supply of UK-produced GM crops on the wider supply chain was examined through an extensive literature review and widespread stakeholder consultation with the feed supply chain. The animal feed sector would benefit from cheaper supplies of raw materials if GM crops were grown and, in the future, they might also benefit from crops with enhanced nutritional profile (such as having higher protein levels) becoming available. This would also be beneficial to livestock producers enabling lower production costs and higher margins. Whilst coexistence measures would result in increased costs, it is unlikely that these would cause substantial changes in the feed chain structure. Retailers were not concerned about a future increase in the amount of animal feed coming from GM crops. To conclude, we (the project team) feel that the adoption of currently available and appropriate GM crops in the UK in the years ahead would benefit farmers, consumers and the feed chain without causing environmental damage. Furthermore, unless British farmers are allowed to grow GM crops in the future, the competitiveness of farming in the UK is likely to decline relative to that globally.

[1]  Emilio Rodríguez-Cerezo,et al.  Economic Impact of Dominant GM Crops Worldwide: a Review , 2006 .

[2]  Alexander J. Stein,et al.  Global value of GM rice: a review of expected agronomic and consumer benefits. , 2013, New biotechnology.

[3]  Richard Bennett,et al.  The use of life-cycle assessment to evaluate the environmental impacts of growing genetically modified, nitrogen use-efficient canola. , 2008, Plant biotechnology journal.

[4]  Dirk Inzé,et al.  Gateway vectors for transformation of cereals. , 2013, Trends in plant science.

[5]  Murray Fulton,et al.  THE PRODUCER BENEFITS OF HERBICIDE-RESISTANT CANOLA , 1999 .

[6]  Justus Wesseler,et al.  The Environmental Benefits and Costs of Genetically Modified (GM) Crops , 2011 .

[7]  Beat Keller,et al.  Functional variability of the Lr34 durable resistance gene in transgenic wheat. , 2012, Plant biotechnology journal.

[8]  Ian McFarlane,et al.  The role of transgenic crops in sustainable development. , 2011, Plant biotechnology journal.

[9]  R Kroes,et al.  Safety evaluation and risk assessment of the herbicide Roundup and its active ingredient, glyphosate, for humans. , 2000, Regulatory toxicology and pharmacology : RTP.

[10]  Marko Bohanec,et al.  A qualitative multi-attribute model for economic and ecological assessment of genetically modified crops , 2008 .

[11]  J. Osorio,et al.  Potential changes in the competitiveness of maize growers in Central Chile through the use of transgenic seed (Bt and RR) , 2004 .

[12]  G. Edmeades,et al.  Progress in Achieving and Delivering Drought Tolerance in Maize-An Update , 2013 .

[13]  Jose M. Yorobe,et al.  Economic impact of Bt corn in the Philippines , 2006 .

[14]  Jörg Romeis,et al.  Indirect Effect of a Transgenic Wheat on Aphids through Enhanced Powdery Mildew Resistance , 2012, PloS one.

[15]  N. L. Innes Global Status of Commercialized Biotech/GM Crops: 2005. ISAAA Briefs No. 34. By C. James. Ithaca, NY, USA: ISAAA (2005), pp. 46, US$50.00. ISBN 1-892456-38-9 , 2006, Experimental Agriculture.

[16]  R Arditi,et al.  Detection, identification and geographical distribution of European corn borer larval parasitoids using molecular markers , 2005, Molecular ecology.

[17]  M. Demont,et al.  GM Crops in Europe: How Much Value and for Whom? Les cultures génétiquement modifiées en Europe : quels avantages et pour qui? Genetisch veränderte Feldfrüchte in Europa: Welcher Wert und für wen? , 2007 .

[18]  Stefania Masci,et al.  Comparative proteomic analysis of kernel proteins of two high amylose transgenic durum wheat lines obtained by biolistic and Agrobacterium-mediated transformations , 2013 .

[19]  M. Stoecker,et al.  Improving hybrid seed production in corn with glyphosate-mediated male sterility. , 2014, Pest management science.

[20]  Jim M. Dunwell,et al.  Technologies for biological containment of GM and Non-GM crops , 2005 .

[21]  Jorge Fernandez-Cornejo,et al.  Technology Adoption and Off-Farm Household Income: The Case of Herbicide-Tolerant Soybeans , 2005, Journal of Agricultural and Applied Economics.

[22]  Wilhelm Gruissem,et al.  Nutritional enhancement of rice for human health: the contribution of biotechnology. , 2013, Biotechnology advances.

[23]  Thomas E. Nickson,et al.  Crop Biotechnology ‐ The State of Play , 2007 .

[24]  Peter W. B. Phillips,et al.  Economic benefits of genetically-modified herbicide-tolerant canola for producers. , 2011 .

[25]  E. Rogers,et al.  Diffusion of Innovations , 1964 .

[26]  Steven E. Naranjo,et al.  Bt Crop Effects on Functional Guilds of Non-Target Arthropods: A Meta-Analysis , 2008, PloS one.

[27]  Michele C. Marra,et al.  The Value of Non-Pecuniary Characteristics of Crop Biotechnologies: A New Look at the Evidence , 2006 .

[28]  Robert A. Graybosch,et al.  Transgenic Enhancement of High‐Molecular‐Weight Glutenin Subunit 1Dy10 Concentration: Effects in Wheat Flour Blends and Sponge and Dough Baking , 2013 .

[29]  László Tamás,et al.  Biolistic- and Agrobacterium-mediated transformation protocols for wheat. , 2012, Methods in molecular biology.

[30]  A. Buléon,et al.  Concerted suppression of all starch branching enzyme genes in barley produces amylose-only starch granules , 2012, BMC Plant Biology.

[31]  Igor Kovalchuk,et al.  A Novel Method of Transgene Delivery into Triticale Plants Using the Agrobacterium Transferred DNA-Derived Nano-Complex[W][OA] , 2012, Plant Physiology.

[32]  G. Stephenson,et al.  Influence of herbicide-resistant canola on the environmental impact of weed management. , 2005, Pest management science.

[33]  Francisco Barro,et al.  The Introgression of RNAi Silencing of γ-Gliadins into Commercial Lines of Bread Wheat Changes the Mixing and Technological Properties of the Dough , 2012, PloS one.

[34]  Leonard P. Gianessi,et al.  Herbicide tolerant soybeans: why growers are adopting Roundup Ready varieties , 1999 .

[35]  David Bonnett,et al.  Phenotyping transgenic wheat for drought resistance. , 2012, Journal of experimental botany.

[36]  Elke Richling,et al.  Anthocyanin-rich purple wheat prolongs the life span of Caenorhabditis elegans probably by activating the DAF-16/FOXO transcription factor. , 2013, Journal of agricultural and food chemistry.

[37]  Emilio Rodriguez Cerezo,et al.  International workshop on socio-economic impacts of genetically modified crops co-organised by JRC-IPTS and FAO , 2013 .

[38]  P. J. W. Lutman,et al.  Predicting yield loss due to interference from two weed species using early observations of relative weed leaf area , 1997 .

[39]  J. Dunwell Patent and intellectual property rights issues , 2010 .

[40]  Peter Kareiva,et al.  A Meta-Analysis of Effects of Bt Cotton and Maize on Nontarget Invertebrates , 2007, Science.

[41]  Jigang Han,et al.  Transgenic expression of lactoferrin imparts enhanced resistance to head blight of wheat caused by Fusarium graminearum , 2012, BMC Plant Biology.

[42]  Colin Thirtle,et al.  Assessing the Performance of GM Maize Amongst Smallholders in KwaZulu-Natal, South Africa , 2009 .

[43]  Ryan H. Brown,et al.  Long-term assessment of transgene behavior in barley: Ds-mediated delivery of bar results in robust, stable, and heritable expression , 2013, In Vitro Cellular & Developmental Biology - Plant.

[44]  Olin D. Anderson,et al.  Physical Characteristics of Genetically Altered Wheat Related to Technological Protein Separation , 2013 .

[45]  Fred Gould,et al.  Delaying Corn Rootworm Resistance to Bt Corn , 2012, Journal of economic entomology.

[46]  John C. Bernard,et al.  Performance Results and Characteristics of Adopters of Genetically Engineered Soybeans in Delaware , 2004, Agricultural and Resource Economics Review.

[47]  Steven E. Naranjo,et al.  Impacts of Bt crops on non-target invertebrates and insecticide use patterns , 2009 .

[48]  A. Blennow,et al.  Future Cereal Starch Bioengineering: Cereal Ancestors Encounter Gene Technology and Designer Enzymes , 2013 .

[49]  Justus Wesseler,et al.  Coexistence regulations and agriculture production: A case study of five Bt maize producers in Portugal , 2010 .

[50]  W. E. Grey,et al.  Increased Resistance to Penicillium Seed Rot in Transgenic Wheat Over‐expressing Puroindolines , 2012 .

[51]  C. Wood,et al.  Resistance to Wheat streak mosaic virus generated by expression of an artificial polycistronic microRNA in wheat. , 2012, Plant biotechnology journal.

[52]  Alistair Munro The spatial impact of genetically modified crops , 2008 .

[53]  Chao Bai,et al.  The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints , 2012, Genes & Nutrition.

[54]  Guanming Shi,et al.  Commercialized transgenic traits, maize productivity and yield risk , 2013, Nature Biotechnology.

[55]  É. Várallyay,et al.  Virus-induced gene silencing of Mlo genes induces powdery mildew resistance in Triticum aestivum , 2012, Archives of Virology.

[56]  Stephen Morse,et al.  Environmental impact of genetically modified cotton in South Africa , 2006 .

[57]  B. Young,et al.  Changes in Herbicide Use Patterns and Production Practices Resulting from Glyphosate-Resistant Crops1 , 2006, Weed Technology.

[58]  P. Motavalli,et al.  Impact of genetically modified crops and their management on soil microbially mediated plant nutrient transformations. , 2004, Journal of environmental quality.

[59]  John P. Giesy,et al.  Ecotoxicological Risk Assessment for Roundup ® Herbicide , 2000 .

[60]  J. Carpenter,et al.  Peer-reviewed surveys indicate positive impact of commercialized GM crops , 2010, Nature Biotechnology.

[61]  Mette Lange,et al.  Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules , 2006, Plant Cell Reports.

[62]  Peter R. Shewry,et al.  Iron and zinc complexation in wild-type and ferritin-expressing wheat grain: implications for mineral transport into developing grain , 2013, JBIC Journal of Biological Inorganic Chemistry.

[63]  P. Langridge,et al.  A Transgenic Transcription Factor (TaDREB3) in Barley Affects the Expression of MicroRNAs and Other Small Non-Coding RNAs , 2012, PloS one.

[64]  David S. Bullock,et al.  Roundup Ready Soybean Technology and Farm Production Costs , 2001 .

[65]  Emilio Rodríguez-Cerezo,et al.  Adoption and performance of the first GM crop introduced in EU agriculture: Bt maize in Spain , 2008 .

[66]  Graham Brookes,et al.  Key environmental impacts of global genetically modified (GM) crop use 1996-2011. , 2013, GM crops & food.

[67]  J. Biesmeijer,et al.  Global pollinator declines: trends, impacts and drivers. , 2010, Trends in ecology & evolution.

[68]  E. Delhaize,et al.  Aluminium tolerance of root hairs underlies genotypic differences in rhizosheath size of wheat (Triticum aestivum) grown on acid soil. , 2012, The New phytologist.

[69]  Graham Brookes,et al.  The farm level impact of using Roundup Ready soybeans in Romania , 2003 .

[70]  Zhao Li,et al.  Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat , 2012, Functional & Integrative Genomics.

[71]  Kazunori Okada,et al.  The potential bioproduction of the pharmaceutical agent sakuranetin, a flavonoid phytoalexin in rice , 2012, Bioengineered.

[72]  David I. Gustafson,et al.  Updated Empirical Model of Genetically Modified Maize Grain Production Practices to Achieve European Union Labeling Thresholds , 2006 .

[73]  Graham Brookes,et al.  GM crops : The global economic and environmental impact-The first nine years 1996-2004 , 2005 .

[74]  József Fogarasi,et al.  Ex Ante Impact Assessment under Imperfect Information: Biotechnology in New Member States of the EU , 2008 .

[75]  E. Rogers,et al.  Diffusion of innovations , 1964, Encyclopedia of Sport Management.

[76]  Jorge Fernandez-Cornejo,et al.  Farm-Level Effects of Adopting Herbicide-Tolerant Soybeans in the U.S.A. , 2002, Journal of Agricultural and Applied Economics.

[77]  Graham Brookes,et al.  GM crops: global socio-economic and environmental impacts 1996- 2007 , 2008 .

[78]  Jorge Fernandez-Cornejo,et al.  The Impacts of Adopting Genetically Engineered Crops in the USA: The Case of Bt Corn , 2005 .

[79]  Beat Keller,et al.  Transgenic Pm3 multilines of wheat show increased powdery mildew resistance in the field. , 2012, Plant biotechnology journal.

[80]  R. D'Ovidio,et al.  Transgenic expression of polygalacturonase-inhibiting proteins in Arabidopsis and wheat increases resistance to the flower pathogen Fusarium graminearum. , 2012, Plant biology.

[81]  Chen Hao,et al.  The Impact of Genetically Engineered Crops on Farm Sustainability in the United States , 2010 .

[82]  R. Just,et al.  Stochastic specification of production functions and economic implications , 1978 .

[83]  Clive James,et al.  ISAAA Briefs brief 41 Global status of Commercialized biotech/GM Crops: 2009 , 2009 .

[84]  C Hall,et al.  What have been the farm -level economic impacts of the global cultivation of GM crops? , 2013 .

[85]  Y. Liao,et al.  Inverse effects of Arabidopsis NPR1 gene on fusarium seedling blight and fusarium head blight in transgenic wheat , 2013 .

[86]  Paul Flanagan,et al.  Predicted Willingness of Irish Farmers to Adopt GM Technology , 2009 .

[87]  Christopher P. Bonin,et al.  ’ s Choice Series on the Next Generation of Biotech Crops Bacterial RNA Chaperones Confer Abiotic Stress Tolerance in Plants and Improved Grain Yield in Maize under Water-Limited Conditions [ W ] , 2008 .

[88]  Felicia Wu,et al.  Mycotoxin Reduction in Bt Corn: Potential Economic, Health, and Regulatory Impacts , 2006, Transgenic Research.

[89]  Toby Bruce,et al.  Metabolic engineering of plant-derived (E)-β-farnesene synthase genes for a novel type of aphid-resistant genetically modified crop plants. , 2012, Journal of integrative plant biology.

[90]  Deepak Saxena,et al.  Bacillus thuringiensis (Bt) toxin released from root exudates and biomass of Bt corn has no apparent effect on earthworms, nematodes, protozoa, bacteria, and fungi in soil , 2001 .

[91]  Yongchun Li,et al.  Identification of Changes in Wheat (Triticum aestivum L.) Seeds Proteome in Response to Anti–trx s Gene , 2011, PloS one.

[92]  Olivier Sanvido,et al.  Ecological impacts of genetically modified crops: ten years of field research and commercial cultivation. , 2007, Advances in biochemical engineering/biotechnology.

[93]  M. Qaim The Economics of Genetically Modified Crops , 2009 .

[94]  Richard Bennett,et al.  Environmental and human health impacts of growing genetically modified herbicide-tolerant sugar beet: a life-cycle assessment. , 2004, Plant biotechnology journal.

[95]  Catherine Ravel,et al.  Down-regulation of the TaGW 2 gene by RNA int rference results in decreased gr in size and weight in wheat , 2012 .

[96]  BadeaAna,et al.  Antimicrobial peptides expressed in wheat reduce susceptibility to Fusarium head blight and powdery mildew , 2013 .

[97]  X. Ye,et al.  An R2R3 MYB transcription factor in wheat, TaPIMP1, mediates host resistance to Bipolaris sorokiniana and drought stresses through regulation of defense- and stress-related genes. , 2012, The New phytologist.

[98]  Gynheung An,et al.  Bacterial transposons are co-transferred with T-DNA to rice chromosomes during Agrobacterium-mediated transformation , 2012, Molecules and cells.

[99]  D. M. Beckles,et al.  Starch characteristics of transgenic wheat (Triticum aestivum L.) overexpressing the Dx5 high molecular weight glutenin subunit are substantially equivalent to those in nonmodified wheat. , 2012, Journal of food science.

[100]  Richard Carew,et al.  Assessing the Contribution of Genetic Enhancements and Fertilizer Application Regimes on Canola Yield and Production Risk in Manitoba , 2006 .

[101]  L. Riesgo,et al.  Economic and agronomic impact of commercialized GM crops: a meta-analysis , 2012, The Journal of Agricultural Science.

[102]  Gerald E. Wilde,et al.  Effect of Bt Corn Expressing the Cry3Bb1 Toxin for Corn Rootworm Control on Aboveground Nontarget Arthropods , 2003 .

[103]  R. D'Ovidio,et al.  Functional characterisation of wheat Pgip genes reveals their involvement in the local response to wounding. , 2013, Plant biology.

[104]  Timothy J. Dennehy,et al.  SOURCES, SINKS, AND THE ZONE OF INFLUENCE OF REFUGES FOR MANAGING INSECT RESISTANCE TO Bt CROPS , 2004 .

[105]  Division on Earth,et al.  The Impact of Genetically Engineered Crops on Farm Sustainability in the United States , 2010 .

[106]  Yong Wang,et al.  Optimization of Regeneration System of Tissue Culture and Transformation of 1Dx5 Gene without Markers in Wheat , 2013 .

[107]  Tsutomu Ishimaru,et al.  Transgenic rice seed synthesizing diverse flavonoids at high levels: a new platform for flavonoid production with associated health benefits. , 2013, Plant biotechnology journal.

[108]  Rodriguez Cerezo Emilio,et al.  Adoption and Impact of the First GM Crop Introduced in EU Agriculture: Bt Maize in Spain , 2008 .

[109]  Zhanjing Huang,et al.  Function of the wheat TaSIP gene in enhancing drought and salt tolerance in transgenic Arabidopsis and rice , 2013, Plant Molecular Biology.

[110]  Vibha Srivastava,et al.  Marker-free site-specific gene integration in rice based on the use of two recombination systems. , 2012, Plant biotechnology journal.

[111]  Luigi Cattivelli,et al.  The rice Osmyb4 gene enhances tolerance to frost and improves germination under unfavourable conditions in transgenic barley plants , 2012, Journal of Applied Genetics.

[112]  Wei Hu,et al.  Coexpression of the High Molecular Weight Glutenin Subunit 1Ax1 and Puroindoline Improves Dough Mixing Properties in Durum Wheat (Triticum turgidum L. ssp. durum) , 2012, PloS one.

[113]  Gerald E. Wilde,et al.  Effect of Bt Corn for Corn Rootworm Control on Nontarget Soil Microarthropods and Nematodes , 2003 .

[114]  Vinay Panwar,et al.  Endogenous silencing of Puccinia triticina pathogenicity genes through in planta-expressed sequences leads to the suppression of rust diseases on wheat. , 2013, The Plant journal : for cell and molecular biology.

[115]  W. H. Furtan,et al.  Economics of transgenic herbicide-tolerant canola: The case of western Canada , 1999 .

[116]  Elena Marcela Badea,et al.  ROUNDUP READY SOYBEAN, A ROMANIAN STORY , 2008 .

[117]  R. Lal,et al.  Soil Carbon Sequestration Impacts on Global Climate Change and Food Security , 2004, Science.

[118]  Dirk Inzé,et al.  Brachypodium distachyon promoters as efficient building blocks for transgenic research in maize. , 2012, Journal of experimental botany.

[119]  Ann E. Blechl,et al.  Variant high-molecular-weight glutenin subunits arising from biolistic transformation of wheat , 2013 .

[120]  Yan Lu,et al.  Transgenic wheat expressing Thinopyrum intermedium MYB transcription factor TiMYB2R-1 shows enhanced resistance to the take-all disease , 2013, Journal of experimental botany.

[121]  R. Ahmad,et al.  Physiological and biochemical changes of CBF3 transgenic oat in response to salinity stress. , 2012, Plant science : an international journal of experimental plant biology.

[122]  R. Lupi,et al.  How much does transgenesis affect wheat allergenicity?: Assessment in two GM lines over-expressing endogenous genes. , 2013, Journal of proteomics.

[123]  Peter Langridge,et al.  Optimization of TaDREB3 gene expression in transgenic barley using cold-inducible promoters. , 2013, Plant biotechnology journal.

[124]  P. Masson,et al.  Insights into the regenerative property of plant cells and their receptivity to transgenesis , 2012, Plant signaling & behavior.

[125]  J. Kumlehn,et al.  phiC31 Integrase-Mediated Site-Specific Recombination in Barley , 2012, PloS one.

[126]  Wendy Harwood,et al.  Transgenic barley lines prove the involvement of TaCBF14 and TaCBF15 in the cold acclimation process and in frost tolerance , 2013, Journal of experimental botany.

[127]  Denis Gaudet,et al.  Carbohydrate profiling in seeds and seedlings of transgenic triticale modified in the expression of sucrose:sucrose-1-fructosyltransferase (1-SST) and sucrose:fructan-6-fructosyltransferase (6-SFT). , 2012, Journal of bioscience and bioengineering.

[128]  Carl E. Pray,et al.  A GM subsistence crop in Africa: the case of Bt white maize in South Africa , 2005 .

[129]  Pedro Castañera,et al.  Resistance monitoring of field populations of the corn borers Sesamia nonagrioides and Ostrinia nubilalis after 5 years of Bt maize cultivation in Spain , 2004 .

[130]  S. Warwick,et al.  A decade of herbicide-resistant crops in Canada , 2006 .

[131]  D. Riewe,et al.  The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers , 2013, Transgenic Research.

[132]  Jim M. Dunwell,et al.  Transgenic plants: methods and protocols (methods in molecular biology) , 2012 .

[133]  J. Kovach,et al.  A Method to Measure the Environmental Impact of Pesticides , 1992 .

[134]  Peter W. B. Phillips,et al.  Environmental impacts from herbicide tolerant canola production in Western Canada , 2011 .

[135]  Andriy Tovkach,et al.  Transposon-Mediated Alteration of TaMATE1B Expression in Wheat Confers Constitutive Citrate Efflux from Root Apices[W] , 2012, Plant Physiology.

[136]  P. Khurana,et al.  Cloning, functional characterisation and transgenic manipulation of vitamin E biosynthesis genes of wheat. , 2013, Functional plant biology : FPB.

[137]  Jim M. Dunwell,et al.  Transgenic cereals: Current status and future prospects , 2014 .

[138]  J. Wolf,et al.  Yield gap analysis with local to global relevance—A review , 2013 .

[139]  Philip Jones,et al.  Farmers' interest in growing GM crops in the UK, in the context of a range of on-farm coexistence issues , 2014 .

[140]  Mette Lange,et al.  Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules. , 2012, Methods in molecular biology.

[141]  Francisco J. Areal,et al.  Consequences of a coexistence policy on the adoption of GMHT crops in the European Union , 2012 .

[142]  Peter W. B. Phillips,et al.  The Economic Impact of Herbicide Tolerant Canola in Canada , 2003 .

[143]  Academia Europaea,et al.  Planting the future: opportunities and challenges for using crop genetic improvement technologies for sustainable agriculture , 2013 .