Concentrations of persistent organochlorine compounds in human milk and placenta are higher in Denmark than in Finland.

BACKGROUND A significantly reduced male reproductive health status, including a higher prevalence of cryptorchidism and hypospadias, has been documented in Danish men compared with Finnish men. Exposure to environmental pollutants with endocrine disrupting activities has been suggested as a possible contributing factor. In this study, we investigated whether there was a difference in milk and placental concentrations of persistent organohalogen compounds, between the two countries. METHODS Organohalogens were analysed by high-resolution gas chromatography-high-resolution mass spectrometry in human milk samples from Finland (n = 65) and Denmark (n = 65) and in placentas from Finland (n = 112) and Denmark (n = 168). RESULTS 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (p,p'-DDE) was the dominant pollutant. beta-Hexa-chloro-cyclohexane (beta-HCH), hexachlorobenzene (HCB), endosulfan-I, dieldrin, oxychlordane (OXC), cis-heptachloroepoxide (c-HE) and 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p'-DDT) were the other main organochlorines detected. Danish samples had significantly higher concentrations of p,p'-DDE, p,p'-DDT, beta-HCH, HCB, dieldrin, c-HE and OXC than did the Finnish samples. Levels of organobrominated compounds were very low and most were undetectable in the majority of samples. BB-153 and BB-155 were the most abundant polybromobiphenyl congeners. BB-153 was more abundant in Danish milk samples compared with Finnish samples, whereas BB-155 was more abundant in the Finnish milk. CONCLUSIONS The organochlorine levels were higher in Danish, than in Finnish, samples, suggesting a higher exposure for Danish infants.

[1]  C. Harris,et al.  Factors affecting the transfer of organochlorine pesticide residues to breastmilk. , 2001, Chemosphere.

[2]  M. McGuire,et al.  Dietary fat type influences total milk fat content in lean women. , 2005, The Journal of nutrition.

[3]  M. D. de Alaniz,et al.  Long-chain polyunsaturated fatty acids in breast milk in La Plata, Argentina: relationship with maternal nutritional status. , 2005, Prostaglandins, leukotrienes, and essential fatty acids.

[4]  G. Burdge,et al.  The effect of reduced maternal protein intake during pregnancy on placental lipid composition in the rat: effect of glycine supplementation of the low protein diet , 2004 .

[5]  E. P. Dekoning,et al.  PCB exposure in utero and via breast milk. A review , 2000, Journal of Exposure Analysis and Environmental Epidemiology.

[6]  H. Hühnerfuss,et al.  Chiral Environmental Pollutants , 2001 .

[7]  R. Kimbrough,et al.  Partitioning of polybrominated biphenyls (PBBs) in serum, adipose tissue, breast milk, placenta, cord blood, biliary fluid, and feces. , 1983, Archives of environmental health.

[8]  J. Toppari,et al.  The birth rate of hypospadias in the Turku area in Finland Note , 2001, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[9]  D. Smith,et al.  Worldwide trends in DDT levels in human breast milk. , 1999, International journal of epidemiology.

[10]  D. Kupfer,et al.  Enantioselective metabolism of the endocrine disruptor pesticide methoxychlor by human cytochromes P450 (P450s): major differences in selective enantiomer formation by various P450 isoforms. , 2002, Drug metabolism and disposition: the biological fate of chemicals.

[11]  K. Schramm,et al.  Enantiomeric ratios as an indicator of exposure processes for persistent pollutants in human placentas. , 2006, Chemosphere.

[12]  Y. Jacquemyn,et al.  Distribution of PCBs and organochlorine pesticides in umbilical cord and maternal serum. , 2002, The Science of the total environment.

[13]  J. Toppari,et al.  Difference in prevalence of congenital cryptorchidism in infants between two Nordic countries , 2004, The Lancet.

[14]  G. Blaschke,et al.  [Chromatographic separation of racemic thalidomide and teratogenic activity of its enantiomers (author's transl)]. , 1979, Arzneimittel-Forschung.

[15]  L. Needham,et al.  Analytic considerations for measuring environmental chemicals in breast milk. , 2002, Environmental health perspectives.

[16]  P. Traissac,et al.  Lipid content and essential fatty acid (EFA) composition of mature Congolese breast milk are influenced by mothers' nutritional status: Impact on infants' EFA supply , 1998, European Journal of Clinical Nutrition.

[17]  K. Solomon,et al.  Spatial distribution of polybrominated diphenyl ethers and polybrominated biphenyls in lake trout from the Laurentian Great Lakes. , 2002, Chemosphere.

[18]  K. Norén,et al.  Certain organochlorine and organobromine contaminants in Swedish human milk in perspective of past 20-30 years. , 2000, Chemosphere.

[19]  H. Adami,et al.  Testicular cancer in nine northern european countries , 1994, International journal of cancer.

[20]  J. Jacobson,et al.  The transfer of polychlorinated biphenyls (PCBs) and polybrominated biphenyls (PBBs) across the human placenta and into maternal milk. , 1984, American journal of public health.

[21]  J. Burguera,et al.  Levels of DDT residues in human milk of Venezuelan women from various rural populations. , 1996, The Science of the total environment.

[22]  P. Haggarty Placental regulation of fatty acid delivery and its effect on fetal growth--a review. , 2002, Placenta.

[23]  G. Solomon,et al.  Chemical contaminants in breast milk: time trends and regional variability. , 2002, Environmental health perspectives.

[24]  K. Schramm,et al.  Persistent Pesticides in Human Breast Milk and Cryptorchidism , 2006, Environmental health perspectives.

[25]  H. Buser,et al.  Enantiomer separation of chlordane components and metabolites using chiral high-resolution gas chromatography and detection by mass spectrometric techniques , 1992 .

[26]  M. E. Flores,et al.  Significance of lipid consumption during lactation. , 1996, Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion.

[27]  M. Hardy A comparison of the properties of the major commercial PBDPO/PBDE product to those of major PBB and PCB products. , 2002, Chemosphere.

[28]  B. Specker,et al.  Differences in fatty acid composition of human milk in vegetarian and nonvegetarian women: long-term effect of diet. , 1987, Journal of pediatric gastroenterology and nutrition.

[29]  K. Korach,et al.  Estrogen receptor residues required for stereospecific ligand recognition and activation. , 1997, Molecular endocrinology.

[30]  J. Toppari,et al.  Hypospadias in a cohort of 1072 Danish newborn boys: prevalence and relationship to placental weight, anthropometrical measurements at birth, and reproductive hormone levels at three months of age. , 2005, The Journal of clinical endocrinology and metabolism.

[31]  K. Schramm,et al.  Prenatal organochlorine pesticides in placentas from Finland: exposure of male infants born during 1997-2001. , 2005, Placenta.

[32]  M. Pillai,et al.  DDT and HCH Load in Mothers and Their Infants in Delhi, India , 1996, Bulletin of environmental contamination and toxicology.

[33]  C. Hughes,et al.  Detection of endocrine disrupting chemicals in samples of second trimester human amniotic fluid. , 2000, The Journal of clinical endocrinology and metabolism.

[34]  Jorma Toppari,et al.  Human Breast Milk Contamination with Phthalates and Alterations of Endogenous Reproductive Hormones in Infants Three Months of Age , 2005, Environmental health perspectives.

[35]  K. Schramm,et al.  From mother to child: investigation of prenatal and postnatal exposure to persistent bioaccumulating toxicants using breast milk and placenta biomonitoring. , 2007, Chemosphere.

[36]  R. Letcher,et al.  Metabolism in the toxicokinetics and fate of brominated flame retardants--a review. , 2003, Environment international.

[37]  T. Bidleman,et al.  Using enantiomers to trace pesticide emissions , 1999 .

[38]  Niels Jørgensen,et al.  East-West gradient in semen quality in the Nordic-Baltic area: a study of men from the general population in Denmark, Norway, Estonia and Finland. , 2002, Human reproduction.

[39]  Sharpe,et al.  Male reproductive health and environmental xenoestrogens. , 1996, Environmental health perspectives.

[40]  D. Muir,et al.  Enantiomer-specific activity of o,p'-DDT with the human estrogen receptor. , 2001, Toxicology letters.

[41]  K. Czaja,et al.  Effect of Age and Number of Deliveries on Mean Concentration of Organochlorine Compounds in Human Breast Milk in Poland , 1997, Bulletin of environmental contamination and toxicology.

[42]  S. Waliszewski,et al.  Carry-over of persistent organochlorine pesticides through placenta to fetus. , 2000, Salud publica de Mexico.

[43]  M. Wolff,et al.  Environmental contaminants in human milk* , 2000, Journal of Exposure Analysis and Environmental Epidemiology.

[44]  J. Boer,et al.  Do flame retardants threaten ocean life? , 1998, Nature.