EFFECT OF TOTAL KHAYA SENEGALENSIS (MELIACEAE) BARKS EXTRACTS ON HEPATIC LIBERATION OF GLUCOSE

Background: Increasing the use of traditional therapy is an obvious reality in some country. The bark of Khaya senegalensis is suspected to treat diabetes. That is what this type of work is started. Aims & Objective: The aim of this work is to test the effect of total Khaya senegalensis barks extracts on the hepatic liberation of glucose in vitro. Materials and Methods: The dried extracts obtained from barks powder had been prepared at concentrations of 0, 2, 5, 10 and 20 μg/L and applied on fragments of mice Wistar isolated liver, kept in Mack -Ewen solution. After incubation at 37°C in oven, glucose level in the solution had been measured by spectrophometer, using GOD-PAP® regent, for each extracts concentration at the times T=0, 10, 20, 30, 40, 50 and 60 minutes. Results: Glucose level in the solution had varied in time function from one extracts concentration to another. Khaya senegalensis barks total extracts had reduced hepatic liberation of glucose. This effect had been comparable to the effect of insulin 2,5IU solution used like reference substance in this experimentation. The total extracts of Khaya senegalensis barks could actuate on enzymes implicated in the metabolism of intra hepatocytary glucose. Conclusion: This investigation had been coupled to phytochimic analyses that showed the presence of saponoides, anthracitic derivers and steroids in Khaya senegalensis barks.