Effects of immunization against an autocrine inhibitor of milk secretion in lactating goats.

1. Lactating goats were immunized against the goat's milk protein identified as a feedback inhibitor of lactation (FIL). Immunization was by three treatments during the declining stage of lactation. 2. When antibodies to FIL were consistently detected in milk (in response to the third treatment), the rate of decline in milk secretion was significantly reduced compared with sham‐immunized controls. Such a response was not apparent with the first two treatments when serum but not milk titres of anti‐FIL were raised. 3. When one gland of immunized goats was switched (after the third immunization) from twice‐ to once‐daily milking, the ipsilateral decrease in the rate of milk secretion was reduced significantly compared with sham‐immunized goats. 4. The results are compatible with the hypothesis that the autocrine agent FIL acts during milk accumulation as an inhibitor of milk secretion.

[1]  C. Wilde,et al.  Intercellular Signalling in the Mammary Gland , 2012, Springer US.

[2]  M. Kerr,et al.  Inhibition of constitutive protein secretion from lactating mouse mammary epithelial cells by FIL (feedback inhibitor of lactation), a secreted milk protein. , 1993, Journal of cell science.

[3]  C. Wilde,et al.  Milk yield and mammary function in goats during and after once-daily milking , 1990, Journal of Dairy Research.

[4]  C. Wilde,et al.  The effect of goat milk fractions on synthesis of milk constituents by rabbit mammary explants and on milk yield in vivo. Evidence for autocrine control of milk secretion. , 1987, The Biochemical journal.

[5]  A. Allison,et al.  An adjuvant formulation that selectively elicits the formation of antibodies of protective isotypes and of cell-mediated immunity. , 1986, Journal of immunological methods.

[6]  A. Husband,et al.  The effect of intraperitoneal and intramammary immunization of sheep on the numbers of antibody-containing cells in the mammary gland, and antibody titres in blood serum and mammary secretions. , 1985, Immunology.

[7]  J. Watts,et al.  Evaluation of protein A and a commercial bacterin as vaccines against Staphylococcus aureus mastitis by experimental challenge. , 1985, Journal of dairy science.

[8]  M. Peaker,et al.  Feed‐back control of milk secretion in the goat by a chemical in milk. , 1984, The Journal of physiology.

[9]  S. C. Nickerson,et al.  Distribution, location, and ultrastructure of plasma cells in the uninfected, lactating bovine mammary gland , 1984, Journal of Dairy Research.

[10]  M. Peaker,et al.  The effects of milking thrice instead of twice daily on milk secretion in the goat. , 1983, Quarterly journal of experimental physiology.

[11]  M. Peaker,et al.  Effects of frequent milking on milk secretion during lactation in the goat: relation to factors which limit the rate of secretion. , 1982, Quarterly journal of experimental physiology.

[12]  M. Peaker.  The effect of raised intramammary pressure on mammary function in the goat in relation to the cessation of lactation. , 1980, The Journal of physiology.

[13]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[14]  G. Mcdowell,et al.  Localized Humoral Immunity with Particular Reference to Ruminants , 1974, Transplantation reviews.

[15]  M. Peaker,et al.  The effects of oxytocin and milk removal on milk secretion in the goat , 1971, The Journal of physiology.

[16]  L. Boddy,et al.  Autocrine regulation of milk secretion by a protein in milk. , 1995, The Biochemical journal.

[17]  T. Tomasi,et al.  Fluorescent localization of immunoglobulins in the tissues of the cow. , 1971, Journal of Dairy Science.