Determining the effect of UV-C, high intensity ultrasound and nonthermal atmospheric plasma treatments on reducing the allergenicity of α-casein and whey proteins

Abstract Casein, β-lactoglobulin and α-lactalbumin are major milk protein allergens. The objective of this study was to investigate the effect of high intensity ultrasound, nonthermal atmospheric plasma and UV-C light treatments in reducing the allergenicity of isolated major milk proteins. SDS-PAGE results for ultrasound and plasma treatments showed no noticeable change in gel band intensities for α-casein, β-lactoglobulin and α-lactalbumin, indicating no change in protein concentration. Ci-ELISA analysis showed that there was no significant difference ( p  > 0.05) in IgE binding values for control and treated samples in ultrasound and plasma treatment conditions tested in this study. UV-C treatment for 15 min resulted in reduced intensities of all three protein bands in SDS-PAGE gel. Ci-ELISA of UV-C treated samples showed, significant reduction ( p in vivo clinical trials need to be conducted to confirm this result.

[1]  Massimo Natale,et al.  Cow's milk allergens identification by two-dimensional immunoblotting and mass spectrometry. , 2004, Molecular nutrition & food research.

[2]  S. J. Jadhav,et al.  Lipid Oxidation in Biological and Food Systems , 1995 .

[3]  K. Davies,et al.  Protein damage and degradation by oxygen radicals. III. Modification of secondary and tertiary structure. , 1987, The Journal of biological chemistry.

[4]  Y. Cho,et al.  Effect of γ-Irradiation on the Molecular Properties of Bovine Serum Albumin and β-Lcatoglobulin , 2000 .

[5]  K. Krishnamurthy,et al.  Effects of pulsed UV-light on peanut allergens in extracts and liquid peanut butter. , 2008, Journal of food science.

[6]  E Anklam,et al.  Methods for allergen analysis in food: a review , 2004, Food additives and contaminants.

[7]  Sehee Lee,et al.  Effect of γ-irradiation on the physicochemical properties of soy protein isolate films , 2005 .

[8]  K. Krishnamurthy.  DECONTAMINATION OF MILK AND WATER BY PULSED UV-LIGHT AND INFRARED HEATING , 2006 .

[9]  E. Anklam,et al.  Milk allergens, their characteristics and their detection in food: A review , 2006 .

[10]  A. J..  Protein Damage and Degradation by Oxygen Radicals , 2001 .

[11]  Tetsuya Akitsu,et al.  Plasma sterilization using glow discharge at atmospheric pressure , 2005 .

[12]  M. Byun,et al.  Application of gamma irradiation for inhibition of food allergy , 2002 .

[13]  M. Hanna,et al.  Soy Protein Isolate/Poly(ethylene oxide) Films , 1995 .

[14]  Hao Feng,et al.  Inactivation of Escherichia coli cells with sonication, manosonication, thermosonication, and manothermosonication: Microbial responses and kinetics modeling , 2009 .

[15]  M. Byun,et al.  Effects of gamma radiation on the allergenic and antigenic properties of milk proteins. , 2001, Journal of food protection.

[16]  Aristippos Gennadios,et al.  Ultraviolet Radiation Affects Physical and Molecular Properties of Soy Protein Films , 2008 .

[17]  S. Shriver.  Effect of Selected Nonthermal Processing Methods on the Allergen Reactivity of Atlantic White Shrimp (Litopenaeus setiferus) , 2011 .

[18]  Zhenxing Li,et al.  Effect of high intensity ultrasound on the allergenicity of shrimp , 2006, Journal of Zhejiang University SCIENCE B.

[19]  E. El-Agamy.  The challenge of cow milk protein allergy , 2007 .

[20]  Wayne L. Smith,et al.  Use of pulsed ultraviolet laser light for the cold pasteurization of bovine milk. , 2002, Journal of food protection.

[21]  J. Wal.  Bovine milk allergenicity. , 2004, Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology.

[22]  Mar Villamiel,et al.  Inactivation of Pseudomonas fluorescens and Streptococcus thermophilus in Trypticase® Soy Broth and total bacteria in milk by continuous-flow ultrasonic treatment and conventional heating , 2000 .

[23]  J. Wal,et al.  Influence of thermal processing on the allergenicity of peanut proteins. , 2005, Journal of agricultural and food chemistry.

[24]  Mounir Laroussi,et al.  Low Temperature Plasma-Based Sterilization: Overview and State-of-the-Art , 2005 .

[25]  Alfred L. Loomis,et al.  THE DESTRUCTION OF LUMINOUS BACTERIA BY HIGH FREQUENCY SOUND WAVES , 1929, Journal of bacteriology.