Proteins from melanosomes of mouse and chick pigment cells

Purified subcellular fractions containing melanosomes from B‐16 mouse melanoma were treated with 2% sodium dodecyl sulfate or 0.5 M sodium hydroxide to dissolve protein. Quantitative measurements indicate that each melanosome contains 0.065×10−10 of protein or about 19% by weight. SDS acrylamide gel electrophoresis of proteins from purified melanosomes resolved six polypeptide bands of major density and about 15 minor bands. These results indicate that the melanosome may be more complex than previous genetic, biochemical or morphological evidence had suggested.

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