Detection of bioterror agents in air samples using real‐time PCR

Aims:  To use real‐time PCR for the detection of bacterial bioterror agents in a liquid air sample containing potential airborne interferences, including bacteria, without the need for DNA extraction.

[1]  O. Landt,et al.  Real-time PCR using hybridization probes for the rapid and specific identification of Francisella tularensis subspecies tularensis. , 2007, Molecular and cellular probes.

[2]  S. Asai,et al.  Detection of Coxiella burnetii specific DNA in blood samples from Japanese patients with chronic nonspecific symptoms by nested polymerase chain reaction. , 1998, FEMS immunology and medical microbiology.

[3]  Patricia Cruz,et al.  Detection and enumeration of airborne biocontaminants. , 2004, Current opinion in biotechnology.

[4]  Paula M. Imbro,et al.  A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents. , 2004, Molecular and cellular probes.

[5]  P. E. Granum,et al.  Genetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracis. , 2007, Journal of microbiological methods.

[6]  David B Levin,et al.  Potential for aerosol dissemination of biological weapons: lessons from biological control of insects. , 2003, Biosecurity and bioterrorism : biodefense strategy, practice, and science.

[7]  Anne-Brit Kolstø,et al.  Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis—One Species on the Basis of Genetic Evidence , 2000, Applied and Environmental Microbiology.

[8]  J. Ezzell,et al.  5′ Nuclease PCR Assay To DetectYersinia pestis , 1998, Journal of Clinical Microbiology.

[9]  I. Agranovski,et al.  Using a bioaerosol personal sampler in combination with real-time PCR analysis for rapid detection of airborne viruses. , 2007, Environmental microbiology.

[10]  G. Sundin,et al.  Ultraviolet Radiation (UVR) Sensitivity Analysis and UVR Survival Strategies of a Bacterial Community from the Phyllosphere of Field-Grown Peanut (Arachis hypogeae L.) , 1999, Microbial Ecology.

[11]  G. Mainelis,et al.  Development and calibration of real-time PCR for quantification of airborne microorganisms in air samples , 2006 .

[12]  Nicolas J. Tourasse,et al.  Multilocus Sequence Typing Scheme for Bacteria of the Bacillus cereus Group , 2004, Applied and Environmental Microbiology.

[13]  H. Ellerbrok,et al.  Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii , 2006, BMC Microbiology.

[14]  Kitagawa,et al.  Monitoring impact of in situ biostimulation treatment on groundwater bacterial community by DGGE. , 2000, FEMS microbiology ecology.

[15]  C. Kuske.  Current and emerging technologies for the study of bacteria in the outdoor air. , 2006, Current opinion in biotechnology.

[16]  Gary L Andersen,et al.  Development of a high‐volume aerosol collection system for the identification of air‐borne micro‐organisms , 2002, Letters in applied microbiology.

[17]  C. Kuske,et al.  Composition of Bacillus Species in Aerosols from 11 U.S. Cities , 2006, Journal of forensic sciences.

[18]  M. Skurnik,et al.  Simultaneous real-time PCR detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis , 2007, European Journal of Clinical Microbiology & Infectious Diseases.

[19]  D. Westphal,et al.  Airborne microorganisms in the African desert dust corridor over the mid-Atlantic ridge, Ocean Drilling Program, Leg 209 , 2006 .

[20]  Elaine Ward,et al.  Methods for Integrated Air Sampling and DNA Analysis for Detection of Airborne Fungal Spores , 2001, Applied and Environmental Microbiology.

[21]  J. Seinfeld,et al.  Effect of Angle of Attack on the Performance of an Airborne Counterflow Virtual Impactor , 2005 .

[22]  Alan M. Jones,et al.  Climate factors influencing bacterial count in background air samples , 2005, International journal of biometeorology.

[23]  Jana Kesavan,et al.  Application of flow cytometry for the assessment of preservation and recovery efficiency of bioaerosol samplers spiked with Pantoea agglomerans. , 2007, Environmental science & technology.

[24]  Olfert Landt,et al.  Rapid detection of Yersinia pestis with multiplex real-time PCR assays using fluorescent hybridisation probes. , 2003, FEMS immunology and medical microbiology.

[25]  S Rozen,et al.  Primer3 on the WWW for general users and for biologist programmers. , 2000, Methods in molecular biology.

[26]  C. Cerniglia,et al.  A universal protocol for PCR detection of 13 species of foodborne pathogens in foods , 1997, Journal of applied microbiology.

[27]  K. Ruoff,et al.  Bioterrorism: Implications for the Clinical Microbiologist , 2001, Clinical Microbiology Reviews.

[28]  B. Drasar,et al.  Detection of Brucella melitensis and Brucella abortus by DNA amplification. , 1992, The Journal of tropical medicine and hygiene.

[29]  D. Griffin,et al.  Atmospheric microbiology in the northern Caribbean during African dust events , 2003 .

[30]  S. Klaschik,et al.  Real-Time PCR for Detection and Differentiation of Gram-Positive and Gram-Negative Bacteria , 2002, Journal of Clinical Microbiology.

[31]  W. Nicholson,et al.  Role of the Spore Coat Layers in Bacillus subtilis Spore Resistance to Hydrogen Peroxide, Artificial UV-C, UV-B, and Solar UV Radiation , 2000, Applied and Environmental Microbiology.

[32]  Ronald M Atlas,et al.  Bioterriorism: from threat to reality. , 2002, Annual review of microbiology.

[33]  L. Stetzenbach,et al.  PCR for bioaerosol monitoring: sensitivity and environmental interference , 1995, Applied and environmental microbiology.

[34]  Gunnar Skogan,et al.  Application of sonication to release DNA from Bacillus cereus for quantitative detection by real-time PCR. , 2003, Journal of microbiological methods.

[35]  M. Pandori,et al.  Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction , 2006, BMC infectious diseases.

[36]  Eoin L. Brodie,et al.  Urban aerosols harbor diverse and dynamic bacterial populations , 2007, Proceedings of the National Academy of Sciences.

[37]  Chihshan Li,et al.  Sampling Performance for Bioaerosols by Flow Cytometry with Fluorochrome , 2005 .

[38]  C. Kuske,et al.  Environmental Survey for Four Pathogenic Bacteria and Closely Related Species Using Phylogenetic and Functional Genes * , 2006, Journal of forensic sciences.