Rapid quantitative phase imaging for partially coherent light microscopy.

Partially coherent light provides promising advantages for imaging applications. In contrast to its completely coherent counterpart, it prevents image degradation due to speckle noise and decreases cross-talk among the imaged objects. These facts make attractive the partially coherent illumination for accurate quantitative imaging in microscopy. In this work, we present a non-interferometric technique and system for quantitative phase imaging with simultaneous determination of the spatial coherence properties of the sample illumination. Its performance is experimentally demonstrated in several examples underlining the benefits of partial coherence for practical imagining applications. The programmable optical setup comprises an electrically tunable lens and sCMOS camera that allows for high-speed measurement in the millisecond range.

[1]  Sophie Pfeifer,et al.  Coherent Light Microscopy Imaging And Quantitative Phase Analysis , 2016 .

[2]  J. Rodrigo,et al.  Recovery of Schell-model partially coherent beams. , 2014, Optics letters.

[3]  S. D. Babacan,et al.  White-light diffraction tomography of unlabelled live cells , 2014, Nature Photonics.

[4]  A. Asundi,et al.  High-speed transport-of-intensity phase microscopy with an electrically tunable lens. , 2013, Optics express.

[5]  Benjamin Schmid,et al.  Rapid 3D light-sheet microscopy with a tunable lens. , 2013, Optics express.

[6]  A A Friesem,et al.  Efficient method for controlling the spatial coherence of a laser. , 2013, Optics letters.

[7]  P. Marquet,et al.  Marker-free phase nanoscopy , 2013, Nature Photonics.

[8]  Ibrahim Abdulhalim,et al.  Spatial and temporal coherence effects in interference microscopy and full‐field optical coherence tomography , 2012 .

[9]  R Harder,et al.  High-resolution three-dimensional partially coherent diffraction imaging , 2012, Nature Communications.

[10]  Brandon Redding,et al.  Speckle-free laser imaging using random laser illumination , 2011, Nature Photonics.

[11]  Aydogan Ozcan,et al.  Partially coherent lensfree tomographic microscopy [Invited]. , 2011, Applied optics.

[12]  Gabriel Cristóbal,et al.  Wavefield imaging via iterative retrieval based on phase modulation diversity. , 2011, Optics express.

[13]  Monika Ritsch-Marte,et al.  Quantitative SLM-based Differential Interference Contrast imaging. , 2010, Optics express.

[14]  V. Micó,et al.  Quantitative phase microscopy using defocusing by means of a spatial light modulator. , 2010, Optics express.

[15]  Patrik Langehanenberg,et al.  Application of partially coherent light in live cell imaging with digital holographic microscopy , 2010 .

[16]  C. Fang-Yen,et al.  Optical diffraction tomography for high resolution live cell imaging. , 2009, Optics express.

[17]  N. Callens,et al.  Fast measurements of concentration profiles inside deformable objects in microflows with reduced spatial coherence digital holography. , 2008, Applied optics.

[18]  P. Marquet,et al.  Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer , 2008, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[19]  Yongkeun Park,et al.  Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum , 2008, Proceedings of the National Academy of Sciences.

[20]  Patrik Langehanenberg,et al.  Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces , 2008 .

[21]  A V Martin,et al.  Spatial incoherence in phase retrieval based on focus variation. , 2006, Ultramicroscopy.

[22]  E. Cuche,et al.  Cell refractive index tomography by digital holographic microscopy. , 2006, Optics letters.

[23]  Munther A. Gdeisat,et al.  Fast two-dimensional phase-unwrapping algorithm based on sorting by reliability following a noncontinuous path. , 2002, Applied optics.

[24]  Zeev Zalevsky,et al.  Computation considerations and fast algorithms for calculating the diffraction integral , 1997 .

[25]  K. Nugent,et al.  Rapid quantitative phase imaging using the transport of intensity equation , 1997 .

[26]  R. Gerchberg.  A practical algorithm for the determination of phase from image and diffraction plane pictures , 1972 .

[27]  Allan Carter Schell,et al.  The multiple plate antenna , 1961 .