Nonrandom γ-TuNA-dependent spatial pattern of microtubule nucleation at the Golgi

GDMT (Golgi-derived microtubule) asymmetry is required for polarized cell motility, but its origin is elusive. Combining experimental and computational approaches, we find that GDMTs arise from spatially restricted hotspots that rely on γ-TuNA (γ-TuRC nucleation activator) activity. The nonrandom nucleation pattern underlies GDMT array asymmetry.

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